Microscope Imaging Software

Microscope Imaging Software

Microscope Imaging Software

Microscope imaging software from Leica Microsystems combines microscope, digital camera and accessories into one fully integrated solution.

Read our latest articles about Microscope Imaging Software

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments.

Designing the Future with Novel and Scalable Stem Cell Culture

Visionary biotech start-up Uncommon Bio is tackling one of the world’s biggest health challenges: food sustainability. In this webinar, Stem Cell Scientist Samuel East shows how they make stem cell culture media for cellular agriculture safe and economically viable. See how they achieved a 1000x reduction in media costs and developed animal free, food safe iPSC culture media.

Explore Alzheimer's Spatial Proteome with Big Data

Alzheimer's disease, a genetic and sporadic neurodegenerative condition, leads to cognitive decline in mid to late life, marked by β-amyloid plaques and tau tangles. With limited treatment options, new investigative strategies are crucial. The Cell DIVE multiplexed imaging solution allows examination of Alzheimer's brain tissue, potentially uncovering new research avenues. Here, we showcase the Cell DIVE image viewer, enabling users to access the full Alzheimer's multiplexed dataset directly in their browser.

Dive into Pancreatic Cancer Research with Big Data

Pancreatic cancer, with a mortality rate near 40%, is challenging to treat due to its proximity to major organs. This story explores the complex biology of pancreatic ductal adenocarcinoma (PDAC), examining molecular and spatial determinants of tumor aggression in metabolism, apoptosis, and immunity. Access the full Cell DIVE dataset in your browser to delve deeper into these findings.

Uncover the Hidden Complexity of Colon Cancer with Big Data

Colorectal cancer poses a significant health burden. While surgery is effective initially, some patients develop recurrent secondary disease with poor prognosis, necessitating advanced therapies like immunotherapies. Spatial biology approaches, such as multiplexed imaging with Cell DIVE, can provide crucial insights for developing novel treatments. Access the full Cell DIVE dataset in your browser to explore these findings further through the Minerva image viewer.

How Efficient is your 3D Organoid Imaging and Analysis Workflow?

Organoid models have transformed life science research but optimizing image analysis protocols remains a key challenge. This webinar explores a streamlined workflow for organoid research, starting with real-time 3D cell culture checks. This is followed by high-speed, high-resolution 3D imaging to generate crisp images and cleaner data for accurate AI-based segmentation and quantification of parameters such as growth rate, cell migration and 3D cellular interactions – enabling deeper insights.

Key Factors for Efficient Cleanliness Analysis

An overview of the key factors necessary for technical cleanliness and efficient cleanliness analysis concerning automotive and electronics manufacturing and production is provided in this article.

Transforming Multiplexed 2D Data into Spatial Insights Guided by AI

Aivia 13 handles large 2D images and enables researchers to obtain deep insights into microenvironment surrounding their phenotypes with millions of detected objects and automatic clustering up to 30 markers.

Exploring Subcellular Spatial Phenotypes with SPARCS

Discover spatially resolved CRISPR screening (SPARCS), a platform for microscopy-based genetic screening for spatial subcellular phenotypes at the human genome scale.

Rapid and Reliable Examination of PCBs & PCBAs with Digital Microscopy

Digital microscopes provide users with a convenient and rapid way to acquire high-quality, reliable image data and make quick inspection and analysis of printed circuit boards (PCBs) and assemblies (PCBAs)

Unlocking Insights in Complex and Dense Neuron Images Guided by AI

The latest advancement in Aivia AI image analysis software provides improved soma detection, additional flexibility in neuron tracing, 3D relational measurement including Sholl analysis and more.

AI Microscopy Enables the Efficient Detection of Rare Events

Localization and selective imaging of rare events is key for the investigation of many processes in biological samples. Yet, due to time constraints and complexity, some experiments are not feasible which limits the horizon for new discoveries. A rare event detection workflow based on AI-powered microscopy offers the potential to overcome these limitations by the synergistic fusion of an intelligent sample navigation, image-acquisition tool, and AI-powered image analysis.

Going Beyond Deconvolution

Widefield fluorescence microscopy is often used to visualize structures in life science specimens and obtain useful information. With the use of fluorescent proteins or dyes, discrete specimen components are marked in a highly specific manner. To fully understand a structure, visualizing it in 3 dimensions can be necessary, but certain challenges are faced when doing so with microscopy.

Alternative Fuels and Why Sustainable Solutions are Important

This free on-demand webinar is about the role of alternative fuel vehicles and why sustainable solutions are of increasing importance to the automotive industry.

Technical Cleanliness in the Automotive Industry for Electromobility

This free on-demand webinar covers the increasing focus on technical cleanliness in the automotive industry for electromobility and the VDA 19.1 revision.

Cleanliness Analysis for Particulate Contamination

Devices, products, and their components fabricated in many industries can be quite sensitive to contamination and, as a result, have stringent requirements for technical cleanliness. Measurement systems for automated particle analysis are often exploited for quantitative validation of product and component cleanliness to fulfill the needs of such industries as automotive, aerospace, microelectronics, pharmaceuticals, and medical devices. This report discusses the use of microscopy-based measurement systems for automated particle analysis.

3D Spatial Analysis Using Mica's AI-Enabled Microscopy Software

This video offers practical advice on the extraction of publication grade insights from microscopy images. Our special guest Luciano Lucas (Leica Microsystems) will illustrate how Mica’s AI-enabled software can be used to start the 3D spatial image analysis process. He will then deep dive into two Mica 3D images and explore spatial relationships between the different visible biological elements. The session concludes with the creation of high-fidelity video animations and other publication ready outputs.

Software Tutorial Videos

You want to learn more about the LAS X, Enersight, Aivia or need advice on its application?

Visit our YouTube Channel!

There you will find numerous videos with helpful tutorials as well as tips and tricks on how to use our microscope software.

Leica Microsystems Tutorials

An all-encompassing solution

With an intuitive user interface and straightforward navigation, our microscope imaging software guides the user through their workflow, whether fast image acquisition or sophisticated expert analysis. A range of specific modules allows configuration of the microscope as a dedicated high-performance tool for almost any application.

LAS X  encompasses all microscope solutions for Life Science and Industry applications, offering maximum flexibility. The previous Leica Application Suite continues to be supported.

Core Features

  • Microscope and digital camera configuration and control in a fully integrated manner.
  • Basic annotation tools allow image and calibration data to be added to images.
  • Auto and manual exposure adjustments allow optimized imaging conditions.
  • A thumbnail gallery of acquired images, which can be reviewed quickly and easily.
  • Automatically calibrated images using data read from Leica microscopes and cameras; a scale bar indicates image size.
     

LAS X Industry

  • Observe your sample over the full screen
  • Make streamlined and flexible measurements when doing analysis
  • Enhance your imaging with stitching in X, Y, and Z 
  • Acquire and recall reliable and reproducible results
  • Customize report templates for your needs
     

LAS X Materials Science Modules

  • Allows users to rapidly perform structural analysis of steel and other materials.
  • Enables the advanced analysis of multiple phases and microstructure components.
  • Specifically developed to combine accurate object and area measurements with high flexibility to tailor automated analyses to a user’s particular needs.
  • Offers a significant improvement in ergonomics and aids users in maintaining consistent accuracy in measurements over time.
     

LAS X Life Science

  • Integrates confocal, widefield, stereo, super-resolution, and light-sheet instruments from Leica Microsystems.
  • Create fast overviews and identify the important details instantly with the LAS X Navigator - a GPS for your cells
  • Imaging tasks become intuitive allowing to focus on your research
     

Frequently Asked Questions Microscope Software

The Enersight software can be operated directly on a monitor, mobile device, or with a computer. You can use Enersight On-Screen Display, which is embedded in the hardware of all compatible devices, to operate your microscope or camera after plugging in the power supply, USB stick, mouse, and monitor. To use Enersight Desktop, a PC-based software, download it from the Leica website and connect the device via a USB-C/A cable to a computer. Alternatively, you can use your mobile device to control the microscope or camera via the Enersight Mobile App. To connect a mobile device to a camera, please refer to tutorial clip First steps with Enersight.

The Ivesta 3 (Integrated camera) microscope, Flexacam i5 and c5 microscope cameras, Flexacam C3 camera, and Emspira 3 digital microscope work with Enersight.

With Enersight software platform, you can acquire images, record videos, compare a live image with a reference via one click, perform measurements, annotate live images, create, and use custom overlays, share images, and benefit from user management functionalities that allow you to tailor the Enersight User Interface to your needs.

Life image and video recording in 4k, 1080, or 720 resolutions with up to 60 fps (frames per second) in MP4 format. Image acquisition in 4k, 1080, 720, 12MP or 3MP resolutions with a JPG/JPEG, TIFF, BMP formats.

First, navigate to the Measurement Tab and Calibration, then place a scale on your microscope stage and set the zoom to the lowest value (e.g., 0.6x), click on Create calibration, enter a value for the linear bar in the desired units, and click "Ok". The calibration value for this magnification is now saved. Then, move to the next zoom value (e.g., 1.0x) and repeat the same actions. You can do the calibration for multiple, or all zoom values if needed. A list of all your saved calibration data can be found in the measurement tab. While doing measurements, please make sure your zoom value corresponds to the calibration value you saved for this magnification step.

To create a custom overlay, please go to the Measurement tab, draw the desired shapes, or perform measurements, then save it as an overlay by clicking on the corresponding button. Your overlay will appear in the Overlay tab under Custom overlays. To use it during live imaging, simply click on it. To upload overlays from the computer, click the Upload button in the Overlay Tab. To edit overlays, navigate to the Measurement tab and click on Import annotations. The window with custom overlays will pop up, then select the desired overlay which will be shown in the measurement tab in an editable format. To save your changes, save this overlay again. To use custom overlays for multiple devices and operation modes, simply save it onto a USB stick.

Using Enersight On-Screen Display, you can set up a network folder in which you can save your images. Please refer to tutorial clip Documentation and Sharing. To be able to save files on a network folder via Enersight Desktop, please set the network folder as default in the Gallery tab. To be able to upload files to a network folder via Enersight Mobile, please use your mobile device’s OS capabilities, by clicking on the Share button in the triple-dot menu of your image or video file.

Yes, you turn the live image into a negative using the camera settings. Take a picture, set it as a comparison in the Gallery, change the transparency to the preferred level, and click on Comparison in the menu bar. As a result, two images will appear on the screen. To overlap one image with another, double click twice on the screen. The comparison functionality is available with Enersight OSD and Enersight Desktop.

In the Measurement tab of Enersight, you have the following tools: selection, distance line tools, measurement tolerancing*, angle, circle tool, edge detection (snap-to-edge)*, shape / polygon, point-to-point measurement, consecutive numbering, and text / line / arrow annotations.

*only available with Enersight Desktop and Enersight OSD

Open Enersight OSD and go to the “Network” tab in the settings. Connect your camera to the network in the following ways:

  • via LAN cable with DHCP turned on or manually setting the IP address provided by your IT support; or
  • insert a Wi-Fi dongle into the back, use client mode, scan the SSIDs of your Wi-Fi, select the one you want, enter your username and password and turn on the Wi-Fi.

For information on how to set up the network folder or send pictures by e-mail, see the Youtube video tutorial “Documentation & Sharing”.

The only system requirements for Enersight Desktop are using Windows 10 Pro or Windows 11.

The licenses can be checked up in via the CodeMeter control center (included in the LAS X software package). After having opened the control center, click on WebAdmin. There you will find your licenses. In case you use a hardware dongle, it has to be inserted into the PC.

In case you have a licensing request or you would like to extend the validity of a demo dongle, we require the context file of your dongle. This can be generated by the CodeMeter control center (including the LAS X software package) and contains all relevant information regarding the dongle and the corresponding license modules. Download the instructions.

The context file can be generated by the CodeMeter control center (including the LAS X software package) and contains all relevant information regarding the dongle and the corresponding license modules. Download the instructions.

A dongle is the location which contains the licensing file and therefore licenses the software. This licensing can either be located on your PC (soft dongle) or on a special codemeter USB stick (hard dongle). The hard dongle offers a Plug&Play function and simply has to be inserted into the USB port of the PC which should be licensed.

LAS X software only runs with Windows, but for a MAC we have a dedicated software called ‎Leica Acquire that you could download for free from the Apple store:
https://apps.apple.com/it/app/leica-acquire/id733706983?mt=12

There is no software for Linux.
 

  • Find your LAS license number as well as the order number on the software box that is enclosed to the system.
  • Find your LAS license number as well as the order number on the microscope system on a licensing label, in case the instrument was bought after 2017-04-01.
  • Find your order number on your invoice.
  • Your license number (LASxxxxx) is printed on a label attached to the LAS DVD packaging.

Find a detailed description of LAS X and its modules on the product page.

Please start the online activation again. For this purpose, you require your LAS license number and your order number.

Please start the online activation again. For this purpose, you require your LAS license number and your order number.

Start the online registration again with the assistance of the online registration tool, which is included in your software package. For this procedure, your require your LAS license number and your order number.

You will find your PC Site Code when you click the 'Start' button in Windows, "All programs" > "Leica Applications Suite" > "LAS License for Activation and Registration".

Afterwards, choose the button "Start LAS for license Activation and Registration".

Please start this program. Within the program, select the button "Options" > "Registration and Activation".

Here, you can see the Site Code now.

The PC Site Code is a clearly specified identification number of your PC that will be defined by the implemented hardware.

You will find your LAS license number as well as the order number on the software box that is enclosed to the system.

Furthermore, you will find your LAS license number as well as the order number on the microscope system on a licensing label, in case the instrument was bought after 2017-04-01.

You will find your order number on your invoice.

Download manual "how to activate an offline-PC."

Download manual "how to activate an online-PC".

Live Cell imaging

To perform successful live-cell imaging experiments, using the right platform is critical, and LAS X software forms one of the critical parts in achieving the correct result.

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3D visualization & analysis

LAS X software visualizes samples in 3D allowing 3D analysis for measuring various aspects of intracellular structures such as volumes of nuclei, their surface, or distances and angles between the nuclei.

Read More

Photomanipulation

Photomanipulation tools enable the microscopist to initiate biological events, precisely adjusting sample labeling, biological activity, local chemical environments and in some instances physically destroy parts of their specimen.

Read More

Live Cell imaging

To perform successful live-cell imaging experiments, using the right platform is critical, and LAS X software forms one of the critical parts in achieving the correct result.

Read More

3D visualization & analysis

LAS X software visualizes samples in 3D allowing 3D analysis for measuring various aspects of intracellular structures such as volumes of nuclei, their surface, or distances and angles between the nuclei.

Read More

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