Contact Us
H&E staining image

Clinical Pathology Microscope Solutions

In clinical microscopy, accurate and timely diagnoses are critical in the management and treatment of medical patients. Efficiency therefore plays an important role in the daily routine of clinical pathologists.

Microscopes can help pathologists achieve efficiency in four ways:

  1. Enabling an easy customizable setup up and operation
  2. Making it easy to provide high-quality images that facilitate accurate diagnoses.
  3. Working with cameras that offer TWAIN capability to archive images in a hospital or lab information system.
  4. Creating ergonomic laboratory workstations that reduce strain and tension for clinicians that spend many hours on the microscope each day.
Need Assistance?

Contact us to learn how Leica clinical microscopes can support timely, accurate diagnoses.

H&E staining image

What is clinical pathology?

Clinical pathology is concerned with the diagnosis of infections and disease based on the laboratory analysis, measurement, and quantification of bodily fluids, tissues, and microorganisms. Laboratory medicine is a term often used synonymously with clinical pathology.

What are microscopes used for in clinical pathology?

Clinical pathologists use microscopes to look at bodily fluids like blood, lymph, etc. to find abnormalities. Medical microbiologists, also called clinical microbiologists, look at microorganisms, such as bacteria, fungi, and parasites. Microscopes magnify details in the specimens and microorganisms and, thus, make visible information which could not be discerned with the naked eye.

What kind of microscopes do clinical pathologists use?

Most clinical pathology labs use bright-field microscopes to examine their samples, as this type of microscope usually provides high enough resolution with an illuminated background that helps them to see their area of interest more easily. However, some applications sometimes require more specialized microscope solutions that offer higher performance, such as fluorescence and laser dissection microscopy.

Comparison table of DM1000 - DM3000 microscopes

  DM1000 LED DM2000 & DM2000 LED DM2500 & DM2500 LED DM3000 & DM3000 LED
Occasional use x - - -
Medium to high workload - x x x
Microscope height and height & viewing angle adjustable with eyepieces x x x x
Control knobs in proximity with symmetrical layout - x x x
Adjustable stage height and focus controls x x x x
Adjustable torque for focus knobs - x x x
Multiple viewing system for simultaneous observation by several users - - x -
Quick objective change in switching mode - - - x
Automated lighting management - - - x
Compact and robust design x x x x
  Go to product page Go to product page Go to product page Go to product page

x = included, - = not available

Why do ergonomics matter in clinical pathology?

Many pathologists work long hours with their microscopes, and this means their necks and backs may be physically strained from standing or sitting at an unnatural angle. Hand and arm muscles may feel tense due to small, repetitive movements for focusing and moving the stage. Eyes may also become strained by the microscope illumination.

Musculoskeletal pain may have serious effects. It can impact pathologists’ well-being and quality of life, as well as their productivity and diagnostic efficiency. 

Leica clinical microscopes can support accurate and timely diagnoses as they make workflows more efficient with their ergonomic design and excellent optics.

I am working with Leica microscopes since my pathology training at Ghent University. When using or testing other microscopes, they never have the image quality and ergonomic features that Leica microscopes provide.

Griet Vercauteren

Griet Vercauteren, DVM, Dipl ECVP, VET-PATH bv, Belgium

The role of microscopes in hematology

Hematology is the subset of clinical pathology that concentrates on studying blood-related disorders. It encompasses the examination of blood cells, coagulation factors, and bone marrow.

Microscopy plays a pivotal role by enabling the visualization of blood smears and bone marrow aspirates, aiding in the identification of abnormalities, such as anemia, leukemia, and clotting disorders. This microscopic analysis provides important insights into hematologic conditions, guiding diagnosis, treatment decisions, and monitoring of patient responses to therapy.

Mycobacterium causing tuberculosis

Discovering the hidden in clinical microbiology

Clinical microbiology is a discipline involving the study of microscopic organisms like bacteria, viruses, fungi, and parasites within biological samples to help diagnose and combat infectious diseases. Various types of microscopies, including light electron (EM) and fluorescence microscopes, can be used to visualize these microorganisms.

Advanced Leica microscope solutions enable microbiologists to continually take their work further by identifying pathogens, studying their structures, behaviors, and interactions with host cells, facilitating new discoveries. This microscopic analysis helps improve patient outcomes by enabling timely interventions in medical treatment and the development of targeted therapies against infectious diseases.

Operators can maintain a comfortable position while working using an ergonomic microscope setup.

How comfortable is your microscope?

Your clinical microscope should be adaptable to your needs, not vice versa. To keep physical strain to a minimum, you should be able to:

  • Adjust the height of the microscope to your own height.
  • Choose the right eyepieces and viewing angle to achieve a comfortable position.
  • Rest your arms and hands on the workbench, with the focus knobs within comfortable reach.
  • Easily keep a symmetrical position with aligned shoulders without leaning to the side.
  • Avoid repetitive movements through automated features.
  • Balance the light intensity, even when you change objectives, to help reduce the risk of eye strain.

How does image quality support accurate diagnoses?

Pathologists need to discern subtle color differences, particularly in stained histological specimens such as Hematoxylin and Eosin (H&E) or papanicolaou for pap smears, to be able to make accurate and reliable diagnoses. After a quick overview, changing to a higher magnification should clearly show the more details.

For the particularly high magnifications used in clinical microbiology examinations, oil-immersion objectives are needed to discern details in the specimen. Only high-quality optics enable pathologists to see a clear, crisp image of their specimen so that they can draw the right conclusions from what they observe.

Additionally, with microscope cameras which have TWAIN capability, you can easily store and transfer your images directly to the hospital or laboratory information system, facilitating the creation of reports.

H&E stained tissue specimen.

Differentiating shades of blue and purple

In clinical pathology, technicians often find themselves looking at samples with many similar color shades, making it difficult to see the details. Leica color-corrected optics will give you an image with clearly discernible areas which are free from color fringes, unlike objectives without correction. With the high color fidelity of color-corrected objectives you can be sure that the colors you see through the eyepieces are the exact colors that are present in the specimen.

Leica optics are world-renowned for their image quality and will serve you well, whether you use conventional or oil-immersion objectives. Cameras suitable for Leica pathology microscopes will also reproduce colors present in your specimen accurately. Their large bit depth and high dynamic range are designed to truthfully represent the different colors which are present in your specimen.

H&E stained Tissue Specimen (40x objective).

Stains, such as Hematoxylin and Eosin (H&E) or papanicolaou for pap smears, stain different parts of the specimen, resulting in a variety of color shades and intensities. Pathologists need to discern subtle color differences in stained specimens to be able to make accurate and reliable diagnoses. 

Phase Contrast of Immune Cells: unstained specimen in phase contrast

Phase contrast is an optical contrast technique for making unstained phase objects (e.g. flat cells) visible under the optical microscope. Cells that appear inconspicuous and transparent in brightfield can be viewed in high contrast and rich detail using a phase contrast microscope.

Pathology Microscopy

Analysis of specimens for pathology sometimes requires long hours working with a microscope. The result for the user may be physical discomfort and strain that can lead to reduced efficiency and the risk of less consistent analysis.

Read More

Frequently Asked Questions Clinical Pathology

Show answer What is the toggle mode?

The automated toggle mode of the DM3000 microscope allows users to switch from one objective to another using two buttons which are located near the focus knobs. Any two objectives can be chosen, so that users can switch quickly between low and high magnification, thus, seeing either an overview of the sample or a specific area of interest in detail.

Show answer How can ergonomic microscopes support pathologists?

With ergonomic microscopes, pathologists can work more comfortably and with less interruptions. An upright posture and a symmetrical setup of the microscope helps avoid strain in the back, neck, and shoulders, while ergonomic armrests and adjustable focus knobs prevent muscle tension in the hands and arms. Thus, pathologists can fully concentrate on their work and need fewer breaks to relax their muscles.

Show answer Does Leica Microsystems offer multi-view microscopes?

Yes, we offer DM Multiple Viewing Systems for up to 12 viewing stations.

Related Articles

Read our latest articles about Clinical Pathology

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments.

More Articles
Immunofluorescence image of a mouse enodmetrial organoid stained with CK14 and DAPI

Advancing Uterine Regenerative Therapies with Endometrial Organoids

Prof. Kang's group investigates important factors that determine the uterine microenvironment in which embryo insertion and pregnancy are successfully maintained. They are working to develop new…
Staining is crucial to revealing details in the specimens.

H&E Staining in Microscopy

If we consider the role of microscopy in pathologists’ daily routines, we often think of the diagnosis. While microscopes indeed play a crucial role at this stage of the pathology lab workflow, they…
Acute lymphoblastic leukemia

How to Benefit from Digital Cytopathology

If you have thought of digital cytopathology as characterized by the digitization of glass slides, this webinar with Dr. Alessandro Caputo from the University Hospital of Salerno, Italy will broaden…
Laboratory

Factors to Consider when Selecting Clinical Microscopes

What matters if you would like to purchase a clinical microscope? Learn how to arrive at the best buying decision from our Science Lab Article.
Histopathological sample, 40x magnification

Clinical Microscopy: Considerations on Camera Selection

The need for images in pathology laboratories has significantly increased over the past few years, be it in histopathology, cytology, hematology, clinical microbiology, or other applications. They…
H&E stained specimen, 20x magnification

The Time to Diagnosis is Crucial in Clinical Pathology

Abnormalities in tissues and fluids - that’s what pathologists are looking for when they examine specimens under the microscope. What they see and deduce from their findings is highly influential, as…

Perform Microscopy Analysis for Pathology Ergonomically and Efficiently

The main performance features of a microscope which are critical for rapid, ergonomic, and precise microscopic analysis of pathology specimens are described in this article. Microscopic analysis of…
Mouse liver (immersion fixed)

Epoxy Resin Embedding of Animal and Human Tissues for Pathological Diagnosis and Research

Application Note for Leica EM AMW - The tissues were fixed in the modified Karnovsky fixative generally by immersion overnight (at minimum 4h at room temperature). Then pieces of approx. 1mm3 were cut…
Scroll to top