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Optimization of the Interplay of Optical Components for Aberration Free Microscopy
Optical microscopes are used to magnify objects which are otherwise invisible for the human eye. For this purpose high quality optics is necessary to achieve appropriate resolution. However, besides…
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Primary Beam Splitting Devices for Confocal Microscopes
Current fluorescence microscopy employs incident illumination which requires separation of illumination and emission light. The classical device performing this separation is a color-dependent beam…
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Photoactivatable, Photoconvertible, and Photoswitchable Fluorescent Proteins
Fluorescent proteins (FPs) such as GFP, YFP or DsRed are powerful tools to visualize cellular components in living cells. Nevertheless, there are circumstances when classical FPs reach their limits.…
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Was macht das Pinhole im konfokalen Mikroskop?
Diese kurze einführende Schrift soll die Bedeutung des Pinholes erläutern und ist für Leser gedacht, die nicht allzu viel Zeit mit der Theorie und den Details der konfokalen Mikroskopie verbringen…
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Rodent and Small-Animal Surgery
Learn how you can perform rodent (mouse, rat, hamster) and small-animal surgery efficiently with a microscope for developmental biology and medical research applications by reading this article.
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Navigating Through the Brain
One of the challenges of neurosurgery is orientation at the surgical site. When resecting tumors, removing arteriovenous malformations, or clipping aneurysms, surgeons often have to work near healthy…
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Milestones in Incident Light Fluorescence Microscopy
Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the…
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Porous Ceramics - Sample Preparation for SEM
Application Note for Leica EM RES102 - Ceramic membrane filters with pore sizes down to a few nanometres must be investigated in cross-section with regard to the structure of the pores. The smallest…
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Chronic Inflammation Under the Microscope
In the course of chronic inflammation certain body areas are recurrently inflamed. This goes along with many human diseases. With the help of widefield light microscopy, the underlying processes can…