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Brief Introduction to Glass Knifemaking for Electron and Light Microscope Applications
Glass knives are used in an ultramicrotome to cut ultrathin slices of samples for electron and light microscope applications. For resin and for cryo sections (Tokuyasu samples) the knife edge must be…
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Brief Introduction to Coating Technology for Electron Microscopy
Coating of samples is required in the field of electron microscopy to enable or improve the imaging of samples. Creating a conductive layer of metal on the sample inhibits charging, reduces thermal…
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Handbook of Optical Filters for Fluorescence Microscopy
Fluorescence microscopy and other light-based applications require optical filters that have demanding spectral and physical characteristics. Often, these characteristics are application-specific and…
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Carbon Thickness Evaluation in Electron Microscopy
The coating layers applied and used for electron microscopy imaging are commonly controlled and measured by quartz crystals. These crystals oscillate with a certain frequency (around 6 megahertz when…
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Spectral Detection – How to Define the Spectral Bands that Collect Probe-specific Emission
To specifically collect emission from multiple probes, the light is first separated spatially and then passes through a device that defines a spectral band. Classically, this is a common glass-based…
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Brief Introduction to High-Pressure Freezing
Water is the most abundant cellular constituent and therefore important for preserving cellular ultra-structure. Currently the only way to fix cellular constituents without introducing significant…
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Step by Step Guide for FRAP Experiments
Fluorescence Recovery After Photobleaching (FRAP) has been considered the most widely applied method for observing translational diffusion processes of macromolecules. The resulting information can be…
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Every Clue Counts – Forensics Inconceivable Without Microscopy
There is no crime without clues. They may be obvious, like a cartridge case at the scene of the crime or clear signs of crowbar damage on a door. But sometimes, clues are microscopically small.…
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Perusing Alternatives for Automated Staining of TEM Thin Sections
Contrast in transmission electron microscopy (TEM) is mainly produced by electron scattering at the specimen: Structures that strongly scatter electrons are referred to as electron dense and appear as…