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Science Lab

Science Lab

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Steps of Koehler Illumination

Koehler Illumination: A Brief History and a Practical Set Up in Five Easy Steps

In this article, we will look at the history of the technique of Koehler Illumination in addition to how to adjust the components in five easy steps.
Left: Tissue cells marked with an immunolabel (FITC) illuminated with wide-band UV excitation. Note the tissue structure with blue autofluorescence. Right: Same tissue and same immunostaining with FITC label illuminated with epi-illumination using narrow-band blue (490 nm) light. Note the increased image contrast (Ploem, 1967)

Milestones in Incident Light Fluorescence Microscopy

Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the…
Exchange a fluorescence bulb.

Video Tutorial: How to Change the Bulb of a Fluorescence Lamp Housing

When applying fluorescence microscopy in biological applications, a lamp housing with mercury burner is the most common light source. This video tutorial shows how to change the bulb of a traditional…
Center a fluorescence bulb.

Video Tutorial: How to Align the Bulb of a Fluorescence Lamp Housing

The traditional light source for fluorescence excitation is a fluorescence lamp housing with mercury burner. A prerequisite for achieving bright and homogeneous excitation is the correct centering and…
Jellyfish Aequorea Victoria

Fluorescent Proteins - From the Beginnings to the Nobel Prize

Fluorescent proteins are the fundament of recent fluorescence microscopy and its modern applications. Their discovery and consequent development was one of the most exciting innovations for life…
Section taste buds rabbit, differential interference contrast microscope

Optical Contrast Methods

Optical contrast methods give the potential to easily examine living and colorless specimens. Different microscopic techniques aim to change phase shifts caused by the interaction of light with the…
Transgenic Mouse Embryo, GFP

Fluorescence in Microscopy

Fluorescence microscopy is a special form of light microscopy. It uses the ability of fluorochromes to emit light after being excited with light of a certain wavelength. Proteins of interest can be…
SPERM HY-LITER™ staining of a sexual assault smear slide which was performed by a forensic DNA crime laboratory.

Forensic Detection of Sperm from Sexual Assault Evidence

The impact of modern scientific methods on the analysis of crime scene evidence has dramatically changed many forensic sub-specialties. Arguably one of the most dramatic examples is the impact of…
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