Science Lab
Science Lab
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Porous Ceramics - Sample Preparation for SEM
Application Note for Leica EM RES102 - Ceramic membrane filters with pore sizes down to a few nanometres must be investigated in cross-section with regard to the structure of the pores. The smallest…
Paper Samples - Sample Preparation for SEM
Application Note for Leica EM RES102 - A coated paper sample has been prepared with ion beam slope cutting in order to test the procedure with regard to its applicability. With the use of ion beam…
Bacteria Protocol - Critical Point Drying of E. coli for SEM
Application Note for Leica EM CPD300 - Critical point drying of E. coli with subsequent platinum / palladium coating and SEM analysis. Sample was inserted into a filter disc (Pore size: 16 - 40 μm)…
Studying Caenorhabditis elegans (C. elegans)
Find out how you can image and study C. elegans roundworm model organisms efficiently with a microscope for developmental biology applications from this article.
Ion Beam Polishing of Sample Surfaces - Sample Preparation for SEM
Application Note for Leica EM RES102 - Ion milling can be used to reduce the roughness of sample surfaces. Small angles less than 6° with respect to the sample surface are necessary. The high voltage…
Epoxy Resin Embedding of Animal and Human Tissues for Pathological Diagnosis and Research
Application Note for Leica EM AMW - The tissues were fixed in the modified Karnovsky fixative generally by immersion overnight (at minimum 4h at room temperature). Then pieces of approx. 1mm3 were cut…
Human Blood Cells Protocol
Application Note for Leica EM CPD300 - Life Science Research. Species: Human (Homo sapiens)
Critical point drying of human blood with subsequent platinum / palladium coating and SEM analysis.
Improvement of Metallic Thin Films for HR-SEM by Using DC Magnetron Sputter Coater
Preparation techniques, like several kinds of coating methods play an important role for high resolution scanning electron microscopy (HR-SEM). Nonconductive sample like biological and synthetic…
Workflows & Protocols: How to Isolate Individual Chromosomes with Laser Microdissection
During the first Leica Workshop in Brazil, at the Centro de Energia Nuclear na Agricultura/USP (CENA), the participants learned how to prepare samples for laser microdissection (LMD) using a cryotome.…
