Science Lab
Science Lab
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FluoSync - a Fast & Gentle Method for Unmixing Multicolor Images
In this white paper, we focus on a fast and reliable method for obtaining high-quality multiplex images in fluorescence microscopy. FluoSync combines an existing method for hybrid unmixing with…
Multiplexing through Spectral Separation of 11 Colors
Fluorescence microscopy is a fundamental tool for life science research that has evolved and matured together with the development of multicolor labeling strategies in cells tissues and model…
Multiplexed Imaging Types, Benefits and Applications
Multiplexed imaging is an emerging and exciting way to extract information from human tissue samples by visualizing many more biomarkers than traditional microscopy. By observing many biomarkers…
Multi-Color Caspase 3/7 Assays with Mica
Caspases are involved in apoptosis and can be utilized to determine if cells are undergoing this programmed cell death pathway in so-called caspase assays. These assays can be run by e.g. flow…
How to Target Fluorescent Structures in 3D for Cryo-FIB Milling
This article describes the major steps of the cryo-electron tomography workflow including super-resolution cryo-confocal microscopy. We describe how subcellular structures can be precisely located in…
Multicolor Microscopy: The Importance of Multiplexing
The term multiplexing refers to the use of multiple fluorescent dyes to examine various elements within a sample. Multiplexing allows related components and processes to be observed in parallel,…
A New Method for Convenient and Efficient Multicolor Imaging
The technique combining hyperspectral unmixing and phasor analysis was developed to simplify the process of getting images from a sample labeled with multiple fluorophores. This aggregate method…
Considerations for Multiplex Live Cell Imaging
Simultaneous multicolor imaging for successful experiments: Live-cell imaging experiments are key to understand dynamic processes. They allow us to visually record cells in their living state, without…
Designing your Research Study with Multiplexed IF Imaging
Multiplexed tissue analysis is a powerful technique that allows comparisons of cell-type locations and cell-type interactions within a single fixed tissue sample. It is common for researchers to ask…
