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Science Lab

Science Lab

Das Wissensportal von Leica Microsystems bietet Ihnen Wissens- und Lehrmaterial zu den Themen der Mikroskopie. Die Inhalte sind so konzipiert, dass sie Einsteiger, erfahrene Praktiker und Wissenschaftler gleichermaßen bei ihrem alltäglichen Vorgehen und Experimenten unterstützen. Entdecken Sie interaktive Tutorials und Anwendungsberichte, erfahren Sie mehr über die Grundlagen der Mikroskopie und High-End-Technologien - werden Sie Teil der Science Lab Community und teilen Sie Ihr Wissen!
„Confetti-Mouse“, taken with the 4Tune spectral detector of Leica’s Deep in-vivo Explorer SP8 DIVE.

Laser Beam Shaping for Multicolor Multiphoton Microscopy

Multiphoton Microscopy is one of the current hot topics in life science research. The new Leica TCS SP8 DIVE from Leica Microsystems presents a series of beneficial new innovations, including a freely…
Convalaria

Introduction to Widefield Microscopy

This article gives an introduction to widefield microscopy, one of the most basic and commonly used microscopy techniques. It also shows the basic differences between widefield and confocal…

Optimization of the Interplay of Optical Components for Aberration Free Microscopy

Optical microscopes are used to magnify objects which are otherwise invisible for the human eye. For this purpose high quality optics is necessary to achieve appropriate resolution. However, besides…

Primary Beam Splitting Devices for Confocal Microscopes

Current fluorescence microscopy employs incident illumination which requires separation of illumination and emission light. The classical device performing this separation is a color-dependent beam…
Dronpa chromophore

Photoactivatable, Photoconvertible, and Photoswitchable Fluorescent Proteins

Fluorescent proteins (FPs) such as GFP, YFP or DsRed are powerful tools to visualize cellular components in living cells. Nevertheless, there are circumstances when classical FPs reach their limits.…
Airy-Muster in der Fokusebene.

Was macht das Pinhole im konfokalen Mikroskop?

Diese kurze einführende Schrift soll die Bedeutung des Pinholes erläutern und ist für Leser gedacht, die nicht allzu viel Zeit mit der Theorie und den Details der konfokalen Mikroskopie verbringen…
Comparison when observing a rodent model organism with a Greenough versus CMO (common main objective) stereo microscope for a task like surgery.

Rodent and Small-Animal Surgery

Learn how you can perform rodent (mouse, rat, hamster) and small-animal surgery efficiently with a microscope for developmental biology and medical research applications by reading this article.
Image Guided Surgery

Navigating Through the Brain

One of the challenges of neurosurgery is orientation at the surgical site. When resecting tumors, removing arteriovenous malformations, or clipping aneurysms, surgeons often have to work near healthy…
Left: Tissue cells marked with an immunolabel (FITC) illuminated with wide-band UV excitation. Note the tissue structure with blue autofluorescence. Right: Same tissue and same immunostaining with FITC label illuminated with epi-illumination using narrow-band blue (490 nm) light. Note the increased image contrast (Ploem, 1967)

Milestones in Incident Light Fluorescence Microscopy

Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the…
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