Leica Microsystems: Science Lab

Science Lab

Das Wissensportal von Leica Microsystems bietet Ihnen Wissens- und Lehrmaterial zu den Themen der Mikroskopie. Die Inhalte sind so konzipiert, dass sie Einsteiger, erfahrene Praktiker und Wissenschaftler gleichermaßen bei ihrem alltäglichen Vorgehen und Experimenten unterstützen. Entdecken Sie interaktive Tutorials und Anwendungsberichte, erfahren Sie mehr über die Grundlagen der Mikroskopie und High-End-Technologien - werden Sie Teil der Science Lab Community und teilen Sie Ihr Wissen!

Image of MDCK (Madin-Darby canine kidney) cells taken with phase contrast.

Phase Contrast and Microscopy

This article explains phase contrast, an optical microscopy technique, which reveals fine details of unstained, transparent specimens that are difficult to see with common brightfield illumination.

Phase-contrast image of a MDCK-cell culture and its respective confluency measured by the Mateo TL microscope.

How to Determine Cell Confluency with a Digital Microscope

This article shows how to measure cell confluency in an easy and consistent way with Mateo TL, increasing confidence in downstream experiments.

Microscope image of cultured cells at the bottom of a dish.

How to do a Proper Cell Culture Quick Check

In order to successfully work with mammalian cell lines, they must be grown under controlled conditions and require their own specific growth medium. In addition, to guarantee consistency their growth…

[Translate to German:] Donor (D) and acceptor (A) molecule which participate in FRET (Förster resonance energy transfer).

Was ist FRET mit FLIM (FLIM-FRET)?

Der Beitrag erläutert die FLIM-FRET-Methode, die Resonanzenergietransfer und Fluoreszenz-Lebensdauer-Imaging zur Untersuchung von Protein-Protein Wechselwirkungen kombiniert.

Multi-Color Caspase 3/7 Assays with Mica

Caspases are involved in apoptosis and can be utilized to determine if cells are undergoing this programmed cell death pathway in so-called caspase assays. These assays can be run by e.g. flow…

[Translate to German:] SEM image of the full Li-NMC electrode sample, showing the two porous layers and the metal film at the center of the structure.

Querschnitt-Ionenstrahlfräsen von Batteriekomponenten

Für ein umfassendes Verständnis von Lithiumbatteriesystemen ist eine qualitativ hochwertige Oberflächenpräparation erforderlich, um die innere Struktur und Morphologie zu untersuchen. Aufgrund der…

How to Successfully Perform Live-cell CLEM

The Leica Nano workflow provides a streamlined live-cell CLEM solution for getting insight bout structural changes of cellular components over time. Besides the technical handling described in the…

How to use a Surgical Microscope as an Operating Room Nurse

Surgical microscopes play an essential role in the modern microsurgery procedures. It provides the surgeon, assistant and operating room staff with a magnified and illuminated high-quality image of…

How to improve your Biomarker Discovery Workflow with Laser Microdissection

Biomarkers can be used as indicators of certain diseases, such as cancer. The tumor microenvironment moved into the spotlight in this concern. It is in close interaction with the tumor itself.…