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Science Lab
Science Lab
Das Wissensportal von Leica Microsystems bietet Ihnen Wissens- und Lehrmaterial zu den Themen der Mikroskopie. Die Inhalte sind so konzipiert, dass sie Einsteiger, erfahrene Praktiker und Wissenschaftler gleichermaßen bei ihrem alltäglichen Vorgehen und Experimenten unterstützen. Entdecken Sie interaktive Tutorials und Anwendungsberichte, erfahren Sie mehr über die Grundlagen der Mikroskopie und High-End-Technologien - werden Sie Teil der Science Lab Community und teilen Sie Ihr Wissen!
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![TIRF Image of Tubulin, YFP, penetration depth: 120 mm TIRF Image of Tubulin, YFP, penetration depth: 120 mm](/fileadmin/_processed_/f/d/csm_2_Tubulin-YFP-TIRF-120mm_27ebf2d5ff.jpg)
Applications of TIRF Microscopy in Life Science Research
The special feature of TIRF microscopy is the employment of an evanescent field for fluorophore excitation. Unlike standard widefield fluorescence illumination procedures with arc lamps, LEDs or…
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![Snapshot from a time lapse of a calcium imaging experiment using the ratiometric calcium indicator Fura-2. Snapshot from a time lapse of a calcium imaging experiment using the ratiometric calcium indicator Fura-2.](/fileadmin/_processed_/5/5/csm_Time_lapse_of_calcium_imaging_experiment_using_the_ratiometric_calcium_indicator_Fura-2_b31cdfd025.jpg)
Ratiometric Imaging
Many fundamental functions of a cell strongly depend on delicate, but nevertheless dynamic balances of ions (e.g. calcium, magnesium), voltage potentials and pH between the cell’s cytosol and the…
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Fluorescence Recovery after Photobleaching (FRAP) and its Offspring
FRAP (Fluorescence recovery after photobleaching) can be used to study cellular protein dynamics: For visualization the protein of interest is fused to a fluorescent protein or a fluorescent dye. A…
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Förster Resonance Energy Transfer (FRET)
The Förster Resonance Energy Transfer (FRET) phenomenon offers techniques that allow studies of interactions in dimensions below the optical resolution limit. FRET describes the transfer of the energy…