Science Lab
Science Lab
Bienvenue sur le portail de connaissances de Leica Microsystems. Vous y trouverez des recherches scientifiques et du matériel didactique sur le thème de la microscopie. Le portail aide les débutants, les praticiens expérimentés et les scientifiques dans leur travail quotidien et leurs expériences. Explorez les didacticiels interactifs et les notes d'application, découvrez les bases de la microscopie ainsi que les technologies de pointe. Faites partie de la communauté Science Lab et partagez votre expertise.
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Spectral Detection – How to Define the Spectral Bands that Collect Probe-specific Emission
To specifically collect emission from multiple probes, the light is first separated spatially and then passes through a device that defines a spectral band. Classically, this is a common glass-based…
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Step by Step Guide for FRAP Experiments
Fluorescence Recovery After Photobleaching (FRAP) has been considered the most widely applied method for observing translational diffusion processes of macromolecules. The resulting information can be…
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Fluorescence Correlation Spectroscopy (FCS)
Fluorescence correlation spectroscopy (FCS) measures fluctuations of fluorescence intensity in a sub-femtolitre volume to detect such parameters as the diffusion time, number of molecules or dark…
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The Principles of White Light Laser Confocal Microscopy
The perfect light source for confocal microscopes in biomedical applications has sufficient intensity, tunable color and is pulsed for use in lifetime fluorescence. Furthermore, it should offer means…
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Confocal Optical Section Thickness
Confocal microscopes are employed to optically slice comparably thick samples.
