Leica LMD6 & LMD7 레이저 미세절단 현미경 시스템
완벽한 해부
This list presents current publications in the major application fields for laser microdissection and is updated monthly.
Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.
The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.
Download the complete list of LMD publications in "Downloads"
Single Cell Applications
Method of the Year 2024: spatial proteomics.
Nat Methods 21, 2195–2196 (2024).
https://doi.org/10.1038/s41592-024-02565-3
A new understanding of tissue biology from MS-based proteomics at single-cell resolution
Nordmann TM, Mund A, Mann M
Nat Methods 21, 2220–2222 (2024).
Direct acting antivirals eradicate HCV from the liver quickly in people with HIV but do not fully reverse immune activation
Sachithanandham J, Leep-Lazar J, Quinn J, Bowden K, Balasubramaniam P, Ward K, Ribeiro R, Sulkowski MS, Balagopal A
The Journal of Infectious Diseases, 2024 Dec 2, DOI: 10.1093/infdis/jiae598
DNA Research
D-loop mutations in mitochondrial DNA are a risk factor for chemotherapy resistance in esophageal cancer
Harino T, Tanaka K, Motooka D, Masuike Y, Takahashi T, Yamashita K, Saito T, Yamamoto K, Makino T, Kurokawa Y, Nakajima K, Eguchi H, Doki Y
Sci Rep. 2024 Dec 30;14(1):31653. doi: 10.1038/s41598-024-80226-3.
High frequency of CTNNB1 variants associated with benign and malignant liver tumors in patients with Congenital Porto-Systemic Shunts
Tyraskis A, Zen Y, Strautnieks S, Cook R, Foskett P, De Vito C, Deheragoda M, Quaglia A, Heaton N, Davenport M, Thompson RJ
Liver Cancer 1–23. 28 Dec 2024, ISSN: 2235-1795 (Print), eISSN: 1664-5553, DOI: 10.1159/000543217
Cell of origin and expression profiles of pseudomyxoma peritonei derived from the appendix
Noguchi R, Yamaguchi K, Yano H, Gohda Y, Kiyomatsu T, Ota Y, Igari T, Takahashi N, Ohsugi T, Takane K, Ikenoue T, Niida A, Shimizu E, Yamaguchi R, Miyano S, Imoto S, Furukawa Y
Pathol Res Pract. 2024 Dec 21;266:155776. doi: 10.1016/j.prp.2024.155776.
RNA Research
The spatially informed mFISHseq assay resolves biomarker discordance and predicts treatment response in breast cancer
Paul ED, Huraiová B, Valková N, Matyasovska N, Gábrišová D, Gubová S, Ignačáková H, Ondris T, Gala M, Barroso L, Bendíková S, Bíla J, Buranovská K, Drobná D, Krchňáková Z, Kryvokhyzha M, Lovíšek D, Mamoilyk V, Mancikova V, Vojtaššáková N, Ristová M, Comino-Méndez I, Andrašina I, Morozov P, Tuschl T, Pareja F, Kather JN & Čekan P
Nat Commun 16, 226 (2025 Jan 2).
Methods for Selective Gene Expression Profiling in Single Tertiary Lymphoid Structure Using Laser Capture Microdissection
Gutierrez-Chavez, C., Knockaert, S., Dieu-Nosjean, MC., Goc, J.
Dieu-Nosjean, MC. (eds) Tertiary Lymphoid Structures. Methods in Molecular Biology, vol 2864. Humana, New York, NY (2025).
Laser Capture Microdissection of Tertiary Lymphoid Structures from Formalin-Fixed Paraffin-Embedded Sections of Canine Cutaneous and Subcutaneous Sarcomas for NanoString Direct RNA Counting
Olver, C.S.
Dieu-Nosjean, MC. (eds) Tertiary Lymphoid Structures. Methods in Molecular Biology, vol 2864. Humana, New York, NY (2025).
Loss of SMARCA4 induces sarcomatogenesis through epithelial-mesenchymal transition in ovarian carcinosarcoma
Katayama Y, Iwasaki T, Yamamoto T, Shimada N, Nakashima M, Toya M, Narutomi F, Tomonaga T, Kato K, Oda Y
Cancer Sci. 2024 Dec 23. doi: 10.1111/cas.16423.
Cell of origin and expression profiles of pseudomyxoma peritonei derived from the appendix
Noguchi R, Yamaguchi K, Yano H, Gohda Y, Kiyomatsu T, Ota Y, Igari T, Takahashi N, Ohsugi T, Takane K, Ikenoue T, Niida A, Shimizu E, Yamaguchi R, Miyano S, Imoto S, Furukawa Y
Pathol Res Pract. 2024 Dec 21;266:155776. doi: 10.1016/j.prp.2024.155776.
Proteomics, Lipidomics, Metabolomics
Complement classical and alternative pathway activation contributes to diabetic kidney disease progression: a glomerular proteomics on kidney biopsies
Yang Y, Zhang Y, Li Y, Zhou X, Honda K, Kang D, Wang M, Yang JH, Xia Z, Wei Y, Liu L, Hu R, Takaki T, Xing
Sci Rep. 2025 Jan 2;15(1):495. doi: 10.1038/s41598-024-84900-4.
Development of a sequential laser microdissection tissue cuts workflow for the spatial and quantitative analysis of drugs in fresh frozen tissue sections
Maris E, Jahouh F, Allaerts K, Vreeken R
PLoS One. 2024 Dec 23;19(12):e0312542. doi: 10.1371/journal.pone.0312542. eCollection 2024.
A new understanding of tissue biology from MS-based proteomics at single-cell resolution
Nordmann TM, Mund A, Mann M
Nat Methods 21, 2220–2222 (2024).
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Method of the Year 2024: spatial proteomics.
Nat Methods 21, 2195–2196 (2024).
https://doi.org/10.1038/s41592-024-02565-3
Highlights 2024: scDVP applied for human health improvement
Nordmann et al.
https://www.nature.com/articles/s41586-024-08061-0
Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409
Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8
Fluorescence Applications
The spatially informed mFISHseq assay resolves biomarker discordance and predicts treatment response in breast cancer
Paul ED, Huraiová B, Valková N, Matyasovska N, Gábrišová D, Gubová S, Ignačáková H, Ondris T, Gala M, Barroso L, Bendíková S, Bíla J, Buranovská K, Drobná D, Krchňáková Z, Kryvokhyzha M, Lovíšek D, Mamoilyk V, Mancikova V, Vojtaššáková N, Ristová M, Comino-Méndez I, Andrašina I, Morozov P, Tuschl T, Pareja F, Kather JN & Čekan P
Nat Commun 16, 226 (2025 Jan 2).
Publisher Correction: Novel laser model of optic nerve transection provides valuable insights about the dynamics of optic nerve regeneration
Moulin C, Dvoriantchikova G, Bineshfar N, Swingle B, Martinez G, Groso D, Zhang M, Ivanov D, Pelaez D
Sci Rep. 2024 Nov 29;14(1):29691. doi: 10.1038/s41598-024-80215-6.
Spatial proteomics of single cells and organelles on tissue slides using filter-aided expansion proteomics
Dong Z, Jiang W, Wu C, Chen T, Chen J, Ding X, Zheng S, Piatkevich KD, Zhu Y, Guo T
Nat Commun. 2024 Oct 30;15(1):9378.
Spatial proteomics identifies JAKi as treatment for a lethal skin disease
Nordmann TM, Anderton H, Hasegawa A, Schweizer L, Zhang P, Stadler PC, Sinha A, Metousis A, Rosenberger FA, Zwiebel M, Satoh TK, Anzengruber F, Strauss MT, Tanzer MC, Saito Y, Gong T, Thielert M, Kimura H, Silke N, Rodriguez EH, Restivo G, Nguyen HH, Gross A, Feldmeyer L, Joerg L, Levesque MP, Murray PJ, Ingen-Housz-Oro S, Mund A, Abe R, Silke J, Ji C, French LE, Mann M
Nature. 2024 Oct 16. doi: 10.1038/s41586-024-08061-0.
Cancer Research
The spatially informed mFISHseq assay resolves biomarker discordance and predicts treatment response in breast cancer
Paul ED, Huraiová B, Valková N, Matyasovska N, Gábrišová D, Gubová S, Ignačáková H, Ondris T, Gala M, Barroso L, Bendíková S, Bíla J, Buranovská K, Drobná D, Krchňáková Z, Kryvokhyzha M, Lovíšek D, Mamoilyk V, Mancikova V, Vojtaššáková N, Ristová M, Comino-Méndez I, Andrašina I, Morozov P, Tuschl T, Pareja F, Kather JN & Čekan
Nat Commun 16, 226 (2025 Jan 2).
D-loop mutations in mitochondrial DNA are a risk factor for chemotherapy resistance in esophageal cancer
Harino T, Tanaka K, Motooka D, Masuike Y, Takahashi T, Yamashita K, Saito T, Yamamoto K, Makino T, Kurokawa Y, Nakajima K, Eguchi H, Doki Y
Sci Rep. 2024 Dec 30;14(1):31653. doi: 10.1038/s41598-024-80226-3.
High frequency of CTNNB1 variants associated with benign and malignant liver tumors in patients with Congenital Porto-Systemic Shunts
Tyraskis A, Zen Y, Strautnieks S, Cook R, Foskett P, De Vito C, Deheragoda M, Quaglia A, Heaton N, Davenport M, Thompson RJ
Liver Cancer 1–23. 28 Dec 2024, ISSN: 2235-1795 (Print), eISSN: 1664-5553, DOI: 10.1159/000543217
Loss of SMARCA4 induces sarcomatogenesis through epithelial-mesenchymal transition in ovarian carcinosarcoma
Katayama Y, Iwasaki T, Yamamoto T, Shimada N, Nakashima M, Toya M, Narutomi F, Tomonaga T, Kato K, Oda
Cancer Sci. 2024 Dec 23. doi: 10.1111/cas.16423.
Cell of origin and expression profiles of pseudomyxoma peritonei derived from the appendix
Noguchi R, Yamaguchi K, Yano H, Gohda Y, Kiyomatsu T, Ota Y, Igari T, Takahashi N, Ohsugi T, Takane K, Ikenoue T, Niida A, Shimizu E, Yamaguchi R, Miyano S, Imoto S, Furukawa Y
Pathol Res Pract. 2024 Dec 21;266:155776. doi: 10.1016/j.prp.2024.155776.
Neurosciences
Persistence of spike protein at the skull-meninges-brain axis may contribute to the neurological sequelae of COVID-19
Rong Z, Mai H, Ebert G, Kapoor S, Puelles VG, Czogalla J, Hu S, Su J, Prtvar D, Singh I, Schädler J, Delbridge C, Steinke H, Frenzel H, Schmidt K, Braun C, Bruch G, Ruf V, Ali M, Sühs KW, Nemati M, Hopfner F, Ulukaya S, Jeridi D, Mistretta D, Caliskan ÖS, Wettengel JM, Cherif F, Kolabas ZI, Molbay M, Horvath I, Zhao S, Krahmer N, Yildirim AÖ, Ussar S, Herms J, Huber TB, Tahirovic S, Schwarzmaier SM, Plesnila N, Höglinger G, Ondruschka B, Bechmann I, Protzer U, Elsner M, Bhatia HS, Hellal F, Ertürk A
Cell Host Microbe. 2024 Nov 26:S1931-3128(24)00438-4. doi: 10.1016/j.chom.2024.11.007.
Deep RNA sequencing of human dorsal root ganglion neurons reveals somatosensory mechanisms
Yu H, Luo W
Nat Neurosci (6 Nov 2024).
Leveraging deep single-soma RNA sequencing to explore the neural basis of human somatosensation
Yu H, Nagi SS, Usoskin D, Hu Y, Kupari J, Bouchatta O, Yan H, Cranfill SL, Gautam M, Su Y, Lu Y, Wymer J, Glanz M, Albrecht P, Song H, Ming GL, Prouty S, Seykora J, Wu H, Ma M, Marshall A, Rice FL, Li M, Olausson H, Ernfors P, Luo W
Nat Neurosci. 2024 Nov 4. doi: 10.1038/s41593-024-01794-1.
Clinical/Pathology/Immunology Research Applications
Complement classical and alternative pathway activation contributes to diabetic kidney disease progression: a glomerular proteomics on kidney biopsies
Yang Y, Zhang Y, Li Y, Zhou X, Honda K, Kang D, Wang M, Yang JH, Xia Z, Wei Y, Liu L, Hu R, Takaki T, Xing G
Sci Rep. 2025 Jan 2;15(1):495. doi: 10.1038/s41598-024-84900-4.
Orally administrated acetate inhibits atherosclerosis progression through AMPK activation via GPR43 in plaque macrophages
Wada T, Senokuchi T, Shi Y, Furusho T, Morita Y, Sarie M, Hanatani S, Fukuda K, Ishii N, Matsumura T, Fujiwara Y, Komohara Y, Araki E, Kubota N
Atherosclerosis. 2024 Dec 12;401:119088. doi: 10.1016/j.atherosclerosis.2024.119088.
Direct acting antivirals eradicate HCV from the liver quickly in people with HIV but do not fully reverse immune activation
Sachithanandham J, Leep-Lazar J, Quinn J, Bowden K, Balasubramaniam P, Ward K, Ribeiro R, Sulkowski MS, Balagopal A
The Journal of Infectious Diseases, 2024 Dec 2, DOI: 10.1093/infdis/jiae598
Developmental Research, Epigenetics & Aging
Age-related TFEB downregulation in proximal tubules causes systemic metabolic disorders and occasional apolipoprotein A4-related amyloidosis
Nakamura J, Yamamoto T, Takabatake Y, Namba-Hamano T, Takahashi A, Matsuda J, Minami S, Sakai S, Yonishi H, Maeda S, Matsui S, Kawai H, Matsui I, Yamamuro T, Edahiro R, Takashima S, Takasawa A, Okada Y, Yoshimori T, Ballabio A, Isaka
JCI Insight. 2024 Dec 19:e184451. doi: 10.1172/jci.insight.184451.
Spatiotemporal functions of leukemia inhibitory factor in embryo attachment and implantation chamber formation
Aikawa S, Hiraoka T, Matsuo M, Fukui Y, Fujita H, Saito-Fujita T, Shimizu-Hirota R, Takeda N, Hiratsuka D, He X, Ishizawa C, Iida R, Akaeda S, Harada M, Wada-Hiraike O, Ikawa M, Osuga Y, Hirota Y
Cell Death Discov. 2024 Nov 25;10(1):481. doi: 10.1038/s41420-024-02228-4.
Slc26a2-mediated sulfate metabolism is significant for the tooth development
Yoshida Y, Inubushi T, Yokoyama M, Nag P, Sasaki JI, Oka A, Murotani T, Kani R, Shiraishi Y, Kurosaka H, Takahata Y, Nishimura R, Imazato S, Papagerakis P, Yamashiro T
Dis Model Mech dmm.052107. 7 Nov 2024
Plant Research
RNA Extraction from Rice Immature Embryo Using Laser Capture Microdissection
Tezuka T, Nosaka-Takahashi M, Yoshikawa T, Sato Y
Methods in Molecular Biology, vol 2869. (2025) in: Kawakatsu, T. (eds) Rice.
Dissecting the cellular architecture and genetic circuitry of the soybean seed
Pelletier JM, Chen M, Lin J-Y, Le B, Kirkbride RC, Hur J, Wang T, Chang S-H, Olsona A, Nikolov L, Goldberg RB, and Harada JJ
PNAS, 30 dec 2024, Vol. 122, No. 1, e2416987121
Laser dissection-assisted phloem transcriptomics highlights the metabolic and physiological changes accompanying clubroot disease progression in oilseed rape
Blicharz S, Stefanowicz K, Truman W, Basińska-Barczak A, Singh D, Kasprzewska A, de Diego N, Vrobel O, Ćavar Zeljković S, Tarkowski P, Malinowski R
Plant J. 2024 Nov 22. doi: 10.1111/tpj.17156.
Forensics
Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.
Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807
Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136
General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/
Miscellaneous
Methods for Selective Gene Expression Profiling in Single Tertiary Lymphoid Structure Using Laser Capture Microdissection
Gutierrez-Chavez, C., Knockaert, S., Dieu-Nosjean, MC., Goc, J.
Dieu-Nosjean, MC. (eds) Tertiary Lymphoid Structures. Methods in Molecular Biology, vol 2864. Humana, New York, NY (2025).
Laser Capture Microdissection of Tertiary Lymphoid Structures from Formalin-Fixed Paraffin-Embedded Sections of Canine Cutaneous and Subcutaneous Sarcomas for NanoString Direct RNA Counting
Olver, C.S.
Dieu-Nosjean, MC. (eds) Tertiary Lymphoid Structures. Methods in Molecular Biology, vol 2864. Humana, New York, NY (2025).
Development of a sequential laser microdissection tissue cuts workflow for the spatial and quantitative analysis of drugs in fresh frozen tissue sections
Maris E, Jahouh F, Allaerts K, Vreeken R
PLoS One. 2024 Dec 23;19(12):e0312542. doi: 10.1371/journal.pone.0312542. eCollection 2024.
pH-FISH: coupled microscale analysis of microbial identity and acid-base metabolism in complex biofilm samples
Del Rey YC, Kitzinger K, Lund MB, Schramm A, Meyer RL, Wagner M, Schlafer S
Microbiome. 2024 Dec 20;12(1):266. doi: 10.1186/s40168-024-01977-9.
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Highlight:
Multiple: Laser Microdissection Protocols for Tissue and Cell Isolation
Bio-protocol Selections | 2024
https://en.bio-protocol.org/ebookinfo.aspx?id=23
Live Cell Culture
A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX
A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023
SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416
