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SP8 LIGHTNING Super-resolution live cell imaging in multicolor

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Zebrafish embryo

Zebrafish embryo, development of the lateral line organ. 3D time-lapse using the 8 kHz resonant scanner and a PL APO 40x 1.1 W objective. Image format 1024x512, duration 8 h 28 min. Green: Membranes, GFP, red: Nuclei, tdTomato. Courtesy of Jonas Hartmann, Gilmour Group, EMBL Heidelberg, Germany.

12 h confocal time-lapse video of dividing RPE cells

12 h confocal time-lapse video of dividing RPE cells stably expressing eGFP-tubulin (cyan) and iRFP-Histone H2B (magenta). Courtesy of C. Gliech, Holland Labs, John Hopkins School of Medicine, Baltimore, US. The Holland lab is interested in the molecular mechanisms that control accurate chromosome…

Host pathogen interaction in plant cells

Prestudy: Simultaneous monitoring of multiple organelle markers in leaf of transgenic Arabidopsis thaliana plants, 1024x1024, PL APO 40x 1.1 W. Leaf of Arabidopsis thaliana. Cyan: Nuclear localisation signal (N7), mTurquoise2. Green: Microtubules (MAP4), VENUS. Magenta: Peroxisomes (SKL), tagRFP-T.…

Mouse Colon

Mouse Colon. Role study of chemokine receptor CX3CR1 in intestinal inflammatory diseases. Green: Macrophages, GFP-CX3CR1. B&W: Vasculature, AF 647-CD31. Red: Blood platelets, PE-CD49B. 15 fps, 512 x 512 , xyzt, 375 x 375 µm, z: 1601.6 µm. Duration of recording 3 min 19 sec. Objective: 25x0.95 W…

NAVIGATOR tile scan (3x2) of a whole mount of mouse diaphragm muscle

Blue: Nuclei, B&W: F-actin, Red: Nicotinic acetylcholine receptors, a principal postsynaptic component of neuromuscular junctions. Green: Tyrosine hydroxylase, a marker for sympathetic neurons. The video shows the spatial relationship between muscle fibers, sympathetic neurons, and neuromuscular…

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