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STELLARIS Confocal Microscope Platform
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A Guide to Spatial Biology
What is spatial biology, and how can researchers leverage its tools to meet the growing demands of biological questions in the post-omics era? This article provides a brief overview of spatial biology…
Cutting-Edge Imaging Techniques for GPCR Signaling
With this webinar on-demand enhance your pharmacological research with our webinar on GPCR signaling and explore cutting-edge imaging techniques that aim to understand how GPCR signaling translates…
Notable AI-based Solutions for Phenotypic Drug Screening
Learn about notable optical microscope solutions for phenotypic drug screening using 3D-cell culture, both planning and execution, from this free, on-demand webinar.
Potential of Multiplex Confocal Imaging for Cancer Research and Immunology
Explore the new frontiers of multi-color fluorescent imaging: from image acquisition to analysis
Windows on Neurovascular Pathologies
Discover how innate immunity can sustain deleterious effects following neurovascular pathologies and the technological developments enabling longitudinal studies into these events.
The Power of Reproducibility, Collaboration and New Imaging Technologies
In this webinar you willl learn what impacts reproducibility in microscopy, what resources and initiatives there are to improve education and rigor and reproducibility in microscopy and how…
AI Microscopy Enables the Efficient Detection of Rare Events
Localization and selective imaging of rare events is key for the investigation of many processes in biological samples. Yet, due to time constraints and complexity, some experiments are not feasible…
Five-color FLIM-STED with One Depletion Laser
Webinar on five-color STED with a single depletion laser and fluorescence lifetime phasor separation.
Confocal Imaging of Immune Cells in Tissue Samples
In this webinar, you will discover how to perform 10-color acquisition using a confocal microscope. The challenges of imaged-based approaches to identify skin immune cells. A new pipeline to assess…
Virtual Reality Showcase for STELLARIS Confocal Microscopy Platform
In this webinar, you will discover how to perform 10-color acquisition using a confocal microscope. The challenges of imaged-based approaches to identify skin immune cells. A new pipeline to assess…
Live-Cell Fluorescence Lifetime Multiplexing Using Organic Fluorophores
On-demand video: Imaging more subcellular targets by using fluorescence lifetime multiplexing combined with spectrally resolved detection.
What is FRET with FLIM (FLIM-FRET)?
This article explains the FLIM-FRET method which combines resonance energy transfer and fluorescence lifetime imaging to study protein-protein interactions.
Insights into Vesicle Trafficking
STELLARIS provides integral access to complementary layers of information for dynamic, structural, and mechanistic insights into vesicle trafficking.
Visualizing Protein-Protein Interactions by Non-Fitting and Easy FRET-FLIM Approaches
The Webinar with Dr. Sergi Padilla-Parra is about visualizing protein-protein interaction. He gives insight into non-fitting and easy FRET-FLIM approaches.
Multiplexing through Spectral Separation of 11 Colors
Fluorescence microscopy is a fundamental tool for life science research that has evolved and matured together with the development of multicolor labeling strategies in cells tissues and model…
A Guide to Fluorescence Lifetime Imaging Microscopy (FLIM)
The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.
TauInteraction – Studying Molecular Interactions with TauSense
Fluorescence microscopy constitutes one of the pillars in life sciences and is a tool commonly used to unveil cellular structure and function. A key advantage of fluorescence microscopy resides in the…
Find Relevant Specimen Details from Overviews
Switch from searching image by image to seeing the full overview of samples quickly and identifying the important specimen details instantly with confocal microscopy. Use that knowledge to set up…
How to Prepare your Specimen for Immunofluorescence Microscopy
Immunofluorescence (IF) is a powerful method for visualizing intracellular processes, conditions and structures. IF preparations can be analyzed by various microscopy techniques (e.g. CLSM,…
How Artificial Intelligence Enhances Confocal Imaging
In this article, we show how artificial intelligence (AI) can enhance your imaging experiments. Namely, how Dynamic Signal Enhancement powered by Aivia improves image quality while capturing the…
Spectroscopic Evaluation of Red Blood Cells
Hemoglobinopathies are a major healthcare problem. This study presents a possible diagnostic tool for thalassemia which is based on confocal spectroscopy. This approach exploits spectral detection and…
Visualizing Protein Degradation and Aggregation in the Living Cell
Our guest speaker, Prof Dr Eric Reits, presents his work on neurodegenerative disorders. Reits’ group are experts on the subject of Huntington’s disease and work towards identifying leads for…
Life Beyond the Pixels: Deep Learning Methods for Single Cell Analysis
Our guest speaker Prof Dr Peter Horvath presents his work on single cell-based large-scale microscopy experiments. This novel targeting approach includes the use of machine learning models and…
Live Cell Imaging Gallery
Live cell microscopy techniques are fundamental to get a better understanding of cellular and molecular function. Today, widefield microscopy is the most common technique used to visualize cell…
Super-Resolution Microscopy Image Gallery
Due to the diffraction limit of light, traditional confocal microscopy cannot resolve structures below ~240 nm. Super-resolution microscopy techniques, such as STED, PALM or STORM or some…
Tissue Image Gallery
Visual analysis of animal and human tissues is critical to understand complex diseases such as cancer or neurodegeneration. From basic immunohistochemistry to intravital imaging, confocal microscopy…
Multicolor Image Gallery
Fluorescence multicolor microscopy, which is one aspect of multiplex imaging, allows for the observation and analysis of multiple elements within the same sample – each tagged with a different…
Cancer Research Image Gallery
Fluorescence microscopy allows the study of changes occurring in tissue and cells during cancer development and progression. Techniques such as live cell imaging are critical to understand cancer…
Cell Biology Image Gallery
Cell biology studies the structure, function and behavior of cells, including cell metabolism, cell cycle, and cell signaling. Fluorescence microscopes are an integral part of a cell biologist…
Adding Dimensions to Multiplex Molecular Imaging
Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better understand the underlying cellular and molecular mechanisms of complex…
Benefits of TauContrast to Image Complex Samples
In this interview, Dr. Timo Zimmermann talks about his experience with the application of TauSense tools and their potential for the investigation of demanding samples such as thick samples or…
How Can Immunofluorescence Aid Virology Research?
Modern virology research has become as crucial now as ever before due to the global COVID-19 pandemic. There are many powerful technologies and assays that virologists can apply to their research into…
Obtain Maximum Information from your Specimen with LIGHTNING
LIGHTNING is an adaptive process for extraction of information that reveals fine structures and details, otherwise simply not visible, fully automatically. Unlike traditional technologies, that use a…
Explore Innovative Techniques to Separate Fluorophores with Overlapping Spectra
In this article we explore several strategies you can take to improve the separation of fluorophores and increase the number of fluorescent probes you can distinguish in your sample.
STELLARIS White Light Lasers
When it comes to choosing fluorescent probes for your multi-color experiments, you shouldn’t have to compromise. Now you can advance beyond conventional excitation sources that limit your fluorophore…
TauSense Technology Imaging Tools
Leica Microsystems’ TauSense technology is a set of imaging modes based on fluorescence lifetime. Found at the core of the STELLARIS confocal platform, it will revolutionize your imaging experiments.…
The Power HyD Detector Family
Powerful photon counting detectors on the STELLARIS confocal platform provide improved photon counting, ultra-sensitive imaging and more color options in the NIR spectrum.
Learn how to Remove Autofluorescence from your Confocal Images
Autofluorescence can significantly reduce what you can see in a confocal experiment. This article explores causes of autofluorescence as well as different ways to remove it, from simple media fixes to…
Zebrafish Brain - Whole Organ Imaging at High Resolution
Structural information is key when one seeks to understand complex biological systems, and one of the most complex biological structures is the vertebrate central nervous system. To image a complete…
What is a Spectral Detector (SP Detector)?
The SP detector from Leica Microsystems denotes a compound detection unit for point scanning microscopes, in particular confocal microscopes. The SP detector splits light into up to 5 spectral bands.…
What is a Resonant Scanner?
A resonant scanner is a type of galvanometric mirror scanner that allows fast image acquisition with single-point scanning microscopes (true confocal and multiphoton laser scanning). High acquisition…
What is a Field-of-View Scanner?
A field-of-view scanner is an assembly of galvanometric scanning mirrors used in single-point confocal microscopes that offer the correct optical recording of large field sizes. The field-of-view…
Resolved Field Number (RFN)
The field number (FN) for optical microscopes indicates the field of view (FOV). It corresponds to the area in the intermediate image that is observable through the eyepieces. Although, we cannot…
What is a Tandem Scanner?
A Tandem Scanner is an assembly of two different types of scanning together in one system for true confocal point scanning. The Tandem Scanner consists of a three-mirror scanning base with the…
See More Than Just Your Image
Despite the emergence of new imaging methods in recent years, true 3D resolution is still achieved by Confocal Laser Scanning Microscopy (CLSM). Through a combination of novel, extremely fast scanning…
Which Sensor is the Best for Confocal Imaging?
The Hybrid Photodetectors (HyD) are! Why that is the case is explained in this short Science Lab article.
Primary Beam Splitting Devices for Confocal Microscopes
Current fluorescence microscopy employs incident illumination which requires separation of illumination and emission light. The classical device performing this separation is a color-dependent beam…
Pinhole Effect in Confocal Microscopes
When operating a confocal microscope, or when discussing features and parameters of such a device, we inescapably mention the pinhole and its diameter. This short introductory document is meant to…
Studying Caenorhabditis elegans (C. elegans)
Find out how you can image and study C. elegans roundworm model organisms efficiently with a microscope for developmental biology applications from this article.
From Light to Mind: Sensors and Measuring Techniques in Confocal Microscopy
This article outlines the most important sensors used in confocal microscopy. By confocal microscopy, we mean "True Confocal Scanning", i.e. the technique that illuminates and measures one single…
Video: The White Light Laser – How to Effectively Excite Multiple Fluorophores with a Single Light Source
The Leica White Light Laser produces a continuous spectral output between the wavelengths of 470 and 670 nm. It allows you to select 8 excitation lines from 3 trillion unique combinations for…
Acousto Optics in True Confocal Spectral Microscope Systems
Acousto-optical elements have successfully replaced planar filters in many positions. The white confocal, regarded as the fully spectrally tunable confocal microscope, was not possible without this…
Nobel Prize 2013 in Physiology or Medicine for Discoveries of the Machinery Regulating Vesicle Traffic
On October 7th 2013, The Nobel Assembly at Karolinska Institutet has decided to award The Nobel Prize in Physiology or Medicine 2012 jointly to James E. Rothman, Randy W. Schekman and Thomas C. Südhof…
Handbook of Optical Filters for Fluorescence Microscopy
Fluorescence microscopy and other light-based applications require optical filters that have demanding spectral and physical characteristics. Often, these characteristics are application-specific and…
Spectral Detection – How to Define the Spectral Bands that Collect Probe-specific Emission
To specifically collect emission from multiple probes, the light is first separated spatially and then passes through a device that defines a spectral band. Classically, this is a common glass-based…
Step by Step Guide for FRAP Experiments
Fluorescence Recovery After Photobleaching (FRAP) has been considered the most widely applied method for observing translational diffusion processes of macromolecules. The resulting information can be…
Fluorescence Correlation Spectroscopy (FCS)
Fluorescence correlation spectroscopy (FCS) measures fluctuations of fluorescence intensity in a sub-femtolitre volume to detect such parameters as the diffusion time, number of molecules or dark…
White Light Laser
The perfect light source for confocal microscopes in biomedical applications has sufficient intensity, tunable color and is pulsed for use in lifetime fluorescence. Furthermore, it should offer means…
Confocal Optical Section Thickness
Confocal microscopes are employed to optically slice comparably thick samples.
Fields of Application
Life Science Research
Leica Microsystems’ life science research microscopes support the imaging needs of the scientific community with advanced innovation and technical expertise for the visualization, measurement and…
Advanced Microscopy Techniques
Advanced microscopy techniques: Advanced microscopy techniques encompass both high-resolution and super-resolution imaging techniques. These techniques are primarily used to visualize biological…
