Leica DMI6000 B with Adaptive Focus Control Automated Microscope System
AFC switched on
COS cells were transfected with a Golgi specific GFP variant. The complete microscope system was placed in a climate chamber with a temperature of 37°C. Shortly after starting the acquisition 1ml of an ice cold salt solution (PBS) was added. Notice that over the complete acquisition time no focus…
AFC switched off
COS cells were transfected with a Golgi specific GFP variant. The complete microscope system was placed in a climate chamber with a temperature of 37°C. Shortly after starting the acquisition 1ml of an ice cold salt solution (PBS) was added. At the time point of adding the ice cold PBS focus drift…
TIRF imaging with simultaneous AFC
MDCK cells expressing GFP-p75Courtesy of Prof. Dr. Ralf Jacob and Dr. Alexandra Elli, Institute of Cytobiology and Cytopathology, University of Marburg, Germany.
Time series with DIC contrast method
MDCK cells (Madin Darby Canine Kidney) were imaged over a time period of 10 hours. AFC was switched on.Courtesy of Prof. Dr. Ralf Jacob and Dr. Alexandra Elli, Institute of Cytobiology and Cytopathology, University of Marburg, Germany.
