Leica LMD6 & LMD7 Laser Microdissection Microscopes
Dissection Perfection
This list presents current publications in the major application fields for laser microdissection and is updated monthly.
Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.
The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.
Download the complete list of LMD publications in "Downloads"
Single Cell Applications
Leveraging deep single-soma RNA sequencing to explore the neural basis of human somatosensation
Yu H, Nagi SS, Usoskin D, Hu Y, Kupari J, Bouchatta O, Yan H, Cranfill SL, Gautam M, Su Y, Lu Y, Wymer J, Glanz M, Albrecht P, Song H, Ming GL, Prouty S, Seykora J, Wu H, Ma M, Marshall A, Rice FL, Li M, Olausson H, Ernfors P, Luo W
Nat Neurosci. 2024 Nov 4. doi: 10.1038/s41593-024-01794-1.
The proteomic landscape of proteotoxic stress in a fibrogenic liver disease
Rosenberger FA, Mädler SC, Thorhauge KH, Steigerwald S. Fromme M, Lebedev M, Weiss CAM, Oeller M, Wahle M, Zwiebel M, Schmacke NA, Detlefsen S, Boor P, Kaserman J, Wilson A, Fabián O, Franková S, Krag AA, Strnad P, and Mann M
bioRxiv, 3rd Nov 2024
Spatial proteomics of single cells and organelles on tissue slides using filter-aided expansion proteomics
Dong Z, Jiang W, Wu C, Chen T, Chen J, Ding X, Zheng S, Piatkevich KD, Zhu Y, Guo T
Nat Commun. 2024 Oct 30;15(1):9378.
Spatial proteomics identifies JAKi as treatment for a lethal skin disease
Nordmann TM, Anderton H, Hasegawa A, Schweizer L, Zhang P, Stadler PC, Sinha A, Metousis A, Rosenberger FA, Zwiebel M, Satoh TK, Anzengruber F, Strauss MT, Tanzer MC, Saito Y, Gong T, Thielert M, Kimura H, Silke N, Rodriguez EH, Restivo G, Nguyen HH, Gross A, Feldmeyer L, Joerg L, Levesque MP, Murray PJ, Ingen-Housz-Oro S, Mund A, Abe R, Silke J, Ji C, French LE, Mann M
Nature. 2024 Oct 16. doi: 10.1038/s41586-024-08061-0.
DNA Research
High mitochondrial DNA levels accelerate lung adenocarcinoma progression
Mennuni M, Wilkie SE, Michon P, Alsina D, Filograna R, Lindberg M, Sanin DE, Rosenberger F, Schaaf A, Larsson E, Pearce EL, Larsson NG
Sci Adv. 2024 Nov;10(44):eadp3481. doi: 10.1126/sciadv.adp3481.
Immunotherapy response induces divergent tertiary lymphoid structure morphologies in hepatocellular carcinoma
Shu DH, Ho WJ, Kagohara LT, Girgis A, Shin SM, Danilova L, Lee JW, Sidiropoulos DN, Mitchell S, Munjal K, Howe K, Bendinelli KJ, Kartalia E, Qi H, Mo G, Montagne J, Leatherman JM, Lopez-Vidal TY, Zhu Q, Huff AL, Yuan X, Hernandez A, Coyne EM, Zaidi N, Zabransky DJ, Engle LL, Ogurtsova A, Baretti M, Laheru D, Durham JN, Wang H, Sunshine JC, Johnston RJ, Deutsch JS, Taube JM, Anders RA, Jaffee EM, Fertig EJ, Yarchoan M
Nat Immunol. 2024 Oct 25. doi: 10.1038/s41590-024-01992-w.
Protocol for constructing whole-genome libraries from mini-biopsies by using laser capture microdissection
Xi Y, Zhang S
STAR Protoc. 2024 Oct 15;5(4):103396. doi: 10.1016/j.xpro.2024.103396.
RNA Research
Leveraging deep single-soma RNA sequencing to explore the neural basis of human somatosensation
Yu H, Nagi SS, Usoskin D, Hu Y, Kupari J, Bouchatta O, Yan H, Cranfill SL, Gautam M, Su Y, Lu Y, Wymer J, Glanz M, Albrecht P, Song H, Ming GL, Prouty S, Seykora J, Wu H, Ma M, Marshall A, Rice FL, Li M, Olausson H, Ernfors P, Luo W
Nat Neurosci. 2024 Nov 4. doi: 10.1038/s41593-024-01794-1.
Lymphoid Aggregates in Canine Cutaneous and Subcutaneous Sarcomas: Immunohistochemical and Gene Expression Evidence for Tertiary Lymphoid Structures
Rugh KM, Ashton LV, Schaffer PA, Olver CS
Vet Comp Oncol. 2024 Oct 27. doi: 10.1111/vco.13020.
NDRG1 upregulation by ubiquitin proteasome system dysfunction aggravates neurodegeneration
Hoshino T, Mukai A, Yamashita H, Misawa H, Urushitani M, Tashiro Y, Matsuzawa SI, Takahashi R
Mol Brain. 2024 Oct 23;17(1):77. doi: 10.1186/s13041-024-01150-1.
Proteomics, Lipidomics, Metabolomics
The proteomic landscape of proteotoxic stress in a fibrogenic liver disease
Rosenberger FA, Mädler SC, Thorhauge KH, Steigerwald S. Fromme M, Lebedev M, Weiss CAM, Oeller M, Wahle M, Zwiebel M, Schmacke NA, Detlefsen S, Boor P, Kaserman J, Wilson A, Fabián O, Franková S, Krag AA, Strnad P, and Mann M
bioRxiv, 3rd Nov 2024
In-Depth Proteomic Analysis of Paraffin-Embedded Tissue Samples from Colorectal Cancer Patients Revealed TXNDC17 and SLC8A1 as Key Proteins Associated with the Disease
Montero-Calle A, Garranzo-Asensio M, Poves C, Sanz R, Dziakova J, Peláez-García A, de Los Ríos V, Martinez-Useros J, Fernández-Aceñero MJ, Barderas R
J Proteome Res. 2024 Oct 23. doi: 10.1021/acs.jproteome.3c00749.
Integrating Functional Protein Drug Target Data into a Precision Oncology Molecular Tumor Board
Hunt AL, Randall J, Mansukhani MM, Nyberg K, Nutcharoen A, Davis J, Corgiat B, Mueller C, Melvin S, Sharma M, Johnston L, Swain W, Abulez T, Bateman NW, Maxwell G, Deeken J, Benyounes A, Petricoin EF, Cannon TL, Conrads TP
medRxiv, 18 Oct 2024
Spatial proteomics identifies JAKi as treatment for a lethal skin disease
Nordmann TM, Anderton H, Hasegawa A, Schweizer L, Zhang P, Stadler PC, Sinha A, Metousis A, Rosenberger FA, Zwiebel M, Satoh TK, Anzengruber F, Strauss MT, Tanzer MC, Saito Y, Gong T, Thielert M, Kimura H, Silke N, Rodriguez EH, Restivo G, Nguyen HH, Gross A, Feldmeyer L, Joerg L, Levesque MP, Murray PJ, Ingen-Housz-Oro S, Mund A, Abe R, Silke J, Ji C, French LE, Mann M
Nature. 2024 Oct 16. doi: 10.1038/s41586-024-08061-0.
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Highlights 2024: scDVP applied for human health improvement
Nordmann et al.
https://www.nature.com/articles/s41586-024-08061-0
Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409
Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8
Fluorescence Applications
Novel laser model of optic nerve transection provides valuable insights about the dynamics of optic nerve regeneration
Moulin C, Dvoriantchikova G, Bineshfar N, Swingle B, Martinez G, Groso D, Zhang M, Ivanov D, Pelaez D
Research Square, 2nd Nov 2024
Spatial proteomics of single cells and organelles on tissue slides using filter-aided expansion proteomics
Dong Z, Jiang W, Wu C, Chen T, Chen J, Ding X, Zheng S, Piatkevich KD, Zhu Y, Guo T
Nat Commun. 2024 Oct 30;15(1):9378.
Spatial proteomics identifies JAKi as treatment for a lethal skin disease
Nordmann TM, Anderton H, Hasegawa A, Schweizer L, Zhang P, Stadler PC, Sinha A, Metousis A, Rosenberger FA, Zwiebel M, Satoh TK, Anzengruber F, Strauss MT, Tanzer MC, Saito Y, Gong T, Thielert M, Kimura H, Silke N, Rodriguez EH, Restivo G, Nguyen HH, Gross A, Feldmeyer L, Joerg L, Levesque MP, Murray PJ, Ingen-Housz-Oro S, Mund A, Abe R, Silke J, Ji C, French LE, Mann M
Nature. 2024 Oct 16. doi: 10.1038/s41586-024-08061-0.
Cancer Research
High mitochondrial DNA levels accelerate lung adenocarcinoma progression
Mennuni M, Wilkie SE, Michon P, Alsina D, Filograna R, Lindberg M, Sanin DE, Rosenberger F, Schaaf A, Larsson E, Pearce EL, Larsson NG
Sci Adv. 2024 Nov;10(44):eadp3481. doi: 10.1126/sciadv.adp3481.
Protumoral lipid droplet-loaded macrophages are enriched in human glioblastoma and can be therapeutically targeted
Governa V, de Oliveira KG, Bång-Rudenstam A, Offer S, Cerezo-Magaña M, Li J, Beyer S, Johansson MC, Månsson AS, Edvardsson C, Durmo F, Gustafsson E, Boukredine A, Jeannot P, Schmidt K, Gezelius E, Menard JA, Garza R, Jakobsson J, de Neergaard T, Sundgren PC, Tiihonen AM, Haapasalo H, Rautajoki KJ, Nordenfelt P, Darabi A, Forsberg-Nilsson K, Pietras A, Talbot H, Bengzon J, Belting M
Sci Transl Med. 2024 Oct 30;16(771):eadk1168. doi: 10.1126/scitranslmed.adk1168.
Gamma-glutamyl cyclotransferase, a molecule identified from the invasive front of follicular thyroid carcinoma, is useful for differential diagnosis of follicular thyroid tumors
Mitsuhashi T, Ogasawara T, Nakayama M, Kondo R, Akiba J, Murotani K, Ono T, Sato F, Umeno H and Yano H
Pathology - Research and Practice, (2024) doi:https://doi.org/10.1016/j.prp.2024.155678
Neurosciences
Leveraging deep single-soma RNA sequencing to explore the neural basis of human somatosensation
Yu H, Nagi SS, Usoskin D, Hu Y, Kupari J, Bouchatta O, Yan H, Cranfill SL, Gautam M, Su Y, Lu Y, Wymer J, Glanz M, Albrecht P, Song H, Ming GL, Prouty S, Seykora J, Wu H, Ma M, Marshall A, Rice FL, Li M, Olausson H, Ernfors P, Luo W
Nat Neurosci. 2024 Nov 4. doi: 10.1038/s41593-024-01794-1.
Novel laser model of optic nerve transection provides valuable insights about the dynamics of optic nerve regeneration
Moulin C, Dvoriantchikova G, Bineshfar N, Swingle B, Martinez G, Groso D, Zhang M, Ivanov D, Pelaez D
Research Square, 2nd Nov 2024
Epigenetic regulation by polycomb repressive complex 1 promotes cerebral cavernous malformations
Pham VC, Rödel CJ, Valentino M, Malinverno M, Paolini A, Münch J, Pasquier C, Onyeogaziri FC, Lazovic B, Girard R, Koskimäki J, Hußmann M, Keith B, Jachimowicz D, Kohl F, Hagelkruys A, Penninger JM, Schulte-Merker S, Awad IA, Hicks R, Magnusson PU, Faurobert E, Pagani M, Abdelilah-Seyfried S
EMBO Mol Med. 2024 Oct 14. doi: 10.1038/s44321-024-00152-9.
Clinical/Pathology/Immunology Research Applications
The proteomic landscape of proteotoxic stress in a fibrogenic liver disease
Rosenberger FA, Mädler SC, Thorhauge KH, Steigerwald S. Fromme M, Lebedev M, Weiss CAM, Oeller M, Wahle M, Zwiebel M, Schmacke NA, Detlefsen S, Boor P, Kaserman J, Wilson A, Fabián O, Franková S, Krag AA, Strnad P, and Mann M
bioRxiv, 3rd Nov 2024
Integrating Functional Protein Drug Target Data into a Precision Oncology Molecular Tumor Board
Hunt AL, Randall J, Mansukhani MM, Nyberg K, Nutcharoen A, Davis J, Corgiat B, Mueller C, Melvin S, Sharma M, Johnston L, Swain W, Abulez T, Bateman NW, Maxwell G, Deeken J, Benyounes A, Petricoin EF, Cannon TL, Conrads TP
medRxiv, 18 Oct 2024
Exploring Structural and Molecular Features of Sciatic Nerve Lesions in Diabetic Neuropathy: Unveiling Pathogenic Pathways and Targets
Schwarz D, Marois ML, Sturm V, Peters AS, Longuespée R, Helm D, Schneider M, Eichmüller B, Hidmark AS, Fischer M, Kender Z, Schwab C, Hausser I, Weis J, Dihlmann S, Böckler D, Bendszus M, Heiland S, Herzig S, Nawroth PP, Szendroedi J, Fleming T
Diabetes. 2024 Oct 17:db240493. doi: 10.2337/db24-0493.
Spatial proteomics identifies JAKi as treatment for a lethal skin disease
Nordmann TM, Anderton H, Hasegawa A, Schweizer L, Zhang P, Stadler PC, Sinha A, Metousis A, Rosenberger FA, Zwiebel M, Satoh TK, Anzengruber F, Strauss MT, Tanzer MC, Saito Y, Gong T, Thielert M, Kimura H, Silke N, Rodriguez EH, Restivo G, Nguyen HH, Gross A, Feldmeyer L, Joerg L, Levesque MP, Murray PJ, Ingen-Housz-Oro S, Mund A, Abe R, Silke J, Ji C, French LE, Mann M
Nature. 2024 Oct 16. doi: 10.1038/s41586-024-08061-0.
Developmental Research, Epigenetics & Aging
Cell-cell interaction determines cell fate of mesoderm-derived cell in tongue development through Hh signaling
Kawasaki M, Kawasaki K, Sari FT, Kudo T, Nihara J, Kitamura M, Nagai T, Utama V, Ishida Y, Meguro F, Kesuma A, Fujita A, Nishimura T, Kogure Y, Maruyama S, Tanuma JI, Kakihara Y, Maeda T, Ghafoor S, Khonsari RH, Corre P, Sharpe PT, Cobourne M, Franco B, Ohazama A
Elife. 2024 Oct 11;13:e85042. doi: 10.7554/eLife.85042.
Unraveling the key role of chromatin structure in cancer development through epigenetic landscape characterization of oral cancer
Xue Y, Liu L, Zhang Y, He Y, Wang J, Ma Z, Li TJ, Zhang J, Huang Y, Gao YQ
Mol Cancer. 2024 Sep 6;23(1):190. doi: 10.1186/s12943-024-02100-0.
Collagen-Heparin-FGF2-VEGF Scaffolds Induce a Regenerative Gene Expression Profile in a Fetal Sheep Wound Model
Gansevoort M, Oostendorp C, Bouwman LF, Tiemessen DM, Geutjes PJ, Feitz WFJ, van Kuppevelt TH, Daamen WF
Tissue Eng Regen Med. 2024 Aug 31. doi: 10.1007/s13770-024-00667-9.
Plant Research
Transport and spatio-temporal conversion of sugar facilitate the formation of spatial gradients of starch in wheat caryopse
Zhong Y, Chen Y, Pan M, Li X, Hebelstrup K, Cai J, Zhou Q, Dai T, Cao W, Jiang D
Commun Biol. 2024 Aug 2;7(1):928. doi: 10.1038/s42003-024-06625-4.
Developing a simple and rapid method for cell-specific transcriptome analysis through laser microdissection: insights from citrus rind with broader implications
Mei Z, Zhu K, Yen D, Jia H, Luo W, Ye J, Deng X
Plant Methods. 2024 Jul 27;20(1):113. doi: 10.1186/s13007-024-01242-y.
Soybean symbiotic-nodule zonation and cell differentiation are defined by NIN2 signaling and GH3-dependent auxin homeostasis
Tu T, Gao Z, Li L, Chen J, Ye K, Xu T, Mai S, Han Q, Chen C, Wu S, Dong Y, Chen J, Huang L, Guan Y, Xie F, Chen X
Dev Cell. 2024 Jul 17:S1534-5807(24)00424-6. doi: 10.1016/j.devcel.2024.07.001.
Forensics
Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.
Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807
Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136
General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/
Miscellaneous
Novel laser model of optic nerve transection provides valuable insights about the dynamics of optic nerve regeneration
Moulin C, Dvoriantchikova G, Bineshfar N, Swingle B, Martinez G, Groso D, Zhang M, Ivanov D, Pelaez D
Research Square, 2nd Nov 2024
Spatial proteomics of single cells and organelles on tissue slides using filter-aided expansion proteomics
Dong Z, Jiang W, Wu C, Chen T, Chen J, Ding X, Zheng S, Piatkevich KD, Zhu Y, Guo T
Nat Commun. 2024 Oct 30;15(1):9378.
Single-cell imaging reveals e cient nutrient uptake and growth of microalgae that darken the Greenland Ice Sheet
Halbach L, Kitzinger K, Hansen M, Benning L, Littmann S, Bradley J, Whitehouse M, Olofsson M, Mourot R, Tranter M, Kuypers M, Ellegaard-Jensen L, Anesio A
Research Square, Oct 17th, 2024
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Highlight:
Multiple: Laser Microdissection Protocols for Tissue and Cell Isolation
Bio-protocol Selections | 2024
https://en.bio-protocol.org/ebookinfo.aspx?id=23
Live Cell Culture
A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX
A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023
SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416
