Leica LMD6 & LMD7 Laser Microdissection Microscopes

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Download the complete list of LMD publications in "Downloads"

Single Cell Applications

Deep visual multi-omics profiling reveals mechanisms that underly cancer cell differentiation and aggressiveness in clear cell renal cell carcinoma
Bolck HA, Migh E, Kriston A, Zajac NH, Kreutzer S, Tofu T, Leary P. Kovacs F, Rutishauser D, Pfammatter S, Grossmann J, Lichtfeld C, Buljan M, Rupp NJ, Horvath P, Moch H
bioRxiv, 5. Feb 2025

Transcription of hepatitis B surface antigen shifts from cccDNA to integrated HBV DNA during treatment
Taddese M, Grudda T, Belluccini G, Anderson M, Cloherty G, Hwang HS, Mani M, Lo CM, Esrig N, Sulkowski MS, Sterling RK, Zhang Y, Ribeiro RM, Thomas DL, Thio CL, Balagopal A
J Clin Invest. 2025 Jan 30:e184243. doi: 10.1172/JCI184243.

Deciphering functional tumor-immune crosstalk through highly multiplexed imaging and deep visual proteomics
Zheng X, Mund A, Mann M
Molecular Cell, 14 Jan 2025, ISSN 1097-2765, DOI: 10.1016/j.molcel.2024.12.023 

DNA Research

Transcription of hepatitis B surface antigen shifts from cccDNA to integrated HBV DNA during treatment
Taddese M, Grudda T, Belluccini G, Anderson M, Cloherty G, Hwang HS, Mani M, Lo CM, Esrig N, Sulkowski MS, Sterling RK, Zhang Y, Ribeiro RM, Thomas DL, Thio CL, Balagopal A
J Clin Invest. 2025 Jan 30:e184243. doi: 10.1172/JCI184243.

D-loop mutations in mitochondrial DNA are a risk factor for chemotherapy resistance in esophageal cancer
Harino T, Tanaka K, Motooka D, Masuike Y, Takahashi T, Yamashita K, Saito T, Yamamoto K, Makino T, Kurokawa Y, Nakajima K, Eguchi H, Doki Y
Sci Rep. 2024 Dec 30;14(1):31653. doi: 10.1038/s41598-024-80226-3.

High frequency of CTNNB1 variants associated with benign and malignant liver tumors in patients with Congenital Porto-Systemic Shunts
Tyraskis A, Zen Y, Strautnieks S, Cook R, Foskett P, De Vito C, Deheragoda M, Quaglia A, Heaton N, Davenport M, Thompson RJ
Liver Cancer 1–23. 28 Dec 2024, ISSN: 2235-1795 (Print), eISSN: 1664-5553, DOI: 10.1159/000543217

RNA Research

Neutralizing IL-15 Inhibits Tissue-Damaging Immune Response in Ex Vivo Cultured Untreated Celiac Intestinal Mucosa
Rotondi Aufiero V, Iacomino G, De Chiara G, Picariello E, Iaquinto G, Troncone R, Mazzarella G:
Cells. 2025; 14(3):234. 

Transcription of hepatitis B surface antigen shifts from cccDNA to integrated HBV DNA during treatment
Taddese M, Grudda T, Belluccini G, Anderson M, Cloherty G, Hwang HS, Mani M, Lo CM, Esrig N, Sulkowski MS, Sterling RK, Zhang Y, Ribeiro RM, Thomas DL, Thio CL, Balagopal A
J Clin Invest. 2025 Jan 30:e184243. doi: 10.1172/JCI184243.

GZMK-expressing CD8+ T cells promote recurrent airway inflammatory diseases
Lan F, Li J, Miao W, Sun F, Duan S, Song Y, Yao J, Wang X, Wang C, Liu X, Wang J, Zhang L, Qi H
Nature. 2025 Jan 15. doi: 10.1038/s41586-024-08395-9

Proteomics, Lipidomics, Metabolomics

Deep visual multi-omics profiling reveals mechanisms that underly cancer cell differentiation and aggressiveness in clear cell renal cell carcinoma
Bolck HA, Migh E, Kriston A, Zajac NH, Kreutzer S, Tofu T, Leary P. Kovacs F, Rutishauser D, Pfammatter S, Grossmann J, Lichtfeld C, Buljan M, Rupp NJ, Horvath P, Moch H
bioRxiv, 5. Feb 2025

Polyacetylenes and sesquiterpenes in Chinese traditional herb Atractylodes lancea: biomarkers and synergistic effects in red secretory cavities
Jiang D, Lin H, Liu Z, Qi K, Zhang W, Wang H, Zhang C, Zhu L, Zhu J, Zhang Y, Huang L, Wang S, Pan Y, Guo L
Mol Hortic. 2025 Feb 4;5(1):11. doi: 10.1186/s43897-024-00130-2.

Mass Spectrometry-Based Spatial Multiomics Revealed Bioaccumulation Preference and Region-Specific Responses of PFOS in Mice Cardiac Tissue
Shi R, Chen Y, Wu W, Diao X, Chen L, Liu X, Wu H, Wang J, Zhu L, Cai Z
Environ Sci Technol. 2025 Jan 22. doi: 10.1021/acs.est.4c09874.

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Highlight 2025: 

Making space for spatial biology in the clinic
Nat Biotechnol (6 Jan2025).
https://doi.org/10.1038/s41587-024-02543-2

Highlights 2024: 

Method of the Year 2024: spatial proteomics. 
Nat Methods 21, 2195–2196 (2024). 
https://doi.org/10.1038/s41592-024-02565-3
Nordmann et al.
https://www.nature.com/articles/s41586-024-08061-0

Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8

Fluorescence Applications

Molecularly-guided spatial proteomics captures single-cell identity and heterogeneity of the nervous system
Dutta S, Pang M, Coughlin GM, Gudavalli S, Roukes ML, Chou T-F, Gradinaru V
bioRxiv, 11 Feb 2025

Deciphering functional tumor-immune crosstalk through highly multiplexed imaging and deep visual proteomics
Zheng X, Mund A, Mann 
Molecular Cell, 14 Jan 2025, ISSN 1097-2765, DOI: 10.1016/j.molcel.2024.12.023

The spatially informed mFISHseq assay resolves biomarker discordance and predicts treatment response in breast cancer
Paul ED, Huraiová B, Valková N, Matyasovska N, Gábrišová D, Gubová S, Ignačáková H, Ondris T, Gala M, Barroso L, Bendíková S, Bíla J, Buranovská K, Drobná D, Krchňáková Z, Kryvokhyzha M, Lovíšek D, Mamoilyk V, Mancikova V, Vojtaššáková N, Ristová M, Comino-Méndez I, Andrašina I, Morozov P, Tuschl T, Pareja F, Kather JN & Čekan P
Nat Commun 16, 226 (2025 Jan 2).

Cancer Research

Deep visual multi-omics profiling reveals mechanisms that underly cancer cell differentiation and aggressiveness in clear cell renal cell carcinoma
Bolck HA, Migh E, Kriston A, Zajac NH, Kreutzer S, Tofu T, Leary P. Kovacs F, Rutishauser D, Pfammatter S, Grossmann J, Lichtfeld C, Buljan M, Rupp NJ, Horvath P, Moch H
bioRxiv, 5. Feb 2025

Epigenetic regulation of HOXA2 expression affects tumor progression and predicts breast cancer patient survival
De Palma FDE, Pol JG, Carbonnier V, Scuderi SA, Mannino D, Montégut L, Sauvat A, Perez-Lanzon M, Uribe-Carretero E, Guarracino M, Granata I, Calogero R, Del Monaco V, Montanaro D, Stoll G, Botti G, D'Aiuto M, Baldi A, D'Argenio V, Guigó R, Rezsohazy R, Kroemer G, Maiuri MC, Salvatore F
Cell Death Differ. 2025 Jan 20. doi: 10.1038/s41418-024-01430-2.

A constitutive interferon-high immunophenotype defines response to immunotherapy in colorectal cancer
Acha-Sagredo A, Andrei P, Clayton K, Taggart E, Antoniotti C, Woodman CA, Afrache H, Fourny C, Armero M, Moinudeen HK, Green M, Bhardwaj N, Mikolajczak A, Rodriguez-Lopez M, Crawford M, Connick E, Lim S, Hobson P, Linares J, Ignatova E, Pelka D, Smyth EC, Diamantis N, Sosnowska D, Carullo M, Ciraci P, Bergamo F, Intini R, Nye E, Barral P, Mishto M, Arnold JN, Lonardi S, Cremolini C, Fontana E, Rodriguez-Justo M, Ciccarelli FD
Cancer Cell. 2025 Jan 13:S1535-6108(24)00483-5. doi: 10.1016/j.ccell.2024.12.008.

Neurosciences

Molecularly-guided spatial proteomics captures single-cell identity and heterogeneity of the nervous system
Dutta S, Pang M, Coughlin GM, Gudavalli S, Roukes ML, Chou T-F, Gradinaru V
bioRxiv, 11 Feb 2025

Persistence of spike protein at the skull-meninges-brain axis may contribute to the neurological sequelae of COVID-19
Rong Z, Mai H, Ebert G, Kapoor S, Puelles VG, Czogalla J, Hu S, Su J, Prtvar D, Singh I, Schädler J, Delbridge C, Steinke H, Frenzel H, Schmidt K, Braun C, Bruch G, Ruf V, Ali M, Sühs KW, Nemati M, Hopfner F, Ulukaya S, Jeridi D, Mistretta D, Caliskan ÖS, Wettengel JM, Cherif F, Kolabas ZI, Molbay M, Horvath I, Zhao S, Krahmer N, Yildirim AÖ, Ussar S, Herms J, Huber TB, Tahirovic S, Schwarzmaier SM, Plesnila N, Höglinger G, Ondruschka B, Bechmann I, Protzer U, Elsner M, Bhatia HS, Hellal F, Ertürk A
Cell Host Microbe. 2024 Nov 26:S1931-3128(24)00438-4. doi: 10.1016/j.chom.2024.11.007.

Deep RNA sequencing of human dorsal root ganglion neurons reveals somatosensory mechanisms
Yu H, Luo W
Nat Neurosci (6 Nov 2024). 

Clinical/Pathology/Immunology Research Applications

Membranoproliferative glomerulonephritis with striated ultrastructural deposits with significantly elevated fibrinogen and fibronectin on mass spectrometry analysis: A case report and literature review
Ishida M, Yamamoto S, Iwashige Y, Miyazawa S, Nakata H, Koichi S, Yahata K, Minamiguchi S, Endo Y, Mii A, Shimizu A, Yanagita M
Nephron. 2025 Feb 13:1-17. doi: 10.1159/000544709.

Spatially resolved proteomic signatures of atherosclerotic carotid artery disease
Sinha A, Sachs N, Kratz E, Pauli J, Steigenwald S, Albrecht V, Nordmann T, Ugur E, Rodriguez EH, Engl M-L, Skowronek P, von Scheidt M, Winter H, Branzan D, Schunkert H, Maegdefessel L, Mann M
bioRxiv, 12 Feb 2025

Neutralizing IL-15 Inhibits Tissue-Damaging Immune Response in Ex Vivo Cultured Untreated Celiac Intestinal Mucosa
Rotondi Aufiero V, Iacomino G, De Chiara G, Picariello E, Iaquinto G, Troncone R, Mazzarella G:
Cells. 2025; 14(3):234. 

Mass Spectrometry-Based Spatial Multiomics Revealed Bioaccumulation Preference and Region-Specific Responses of PFOS in Mice Cardiac Tissue
Shi R, Chen Y, Wu W, Diao X, Chen L, Liu X, Wu H, Wang J, Zhu L, Cai Z
Environ Sci Technol. 2025 Jan 22. doi: 10.1021/acs.est.4c09874.

Developmental Research, Epigenetics & Aging

Multi-omics-based mapping of decidualization resistance in patients with a history of severe preeclampsia
Muñoz-Blat I, Pérez-Moraga R, Castillo-Marco N, Cordero T, Ochando A, Ortega-Sanchís S, Parras M, Monfort-Ortiz R, Satorres-Perez E, Novillo B, Perales A, Gormley M, Granados-Aparici S, Noguera R, Roson B, Fisher SJ, Simón C, Garrido-Gómez T
Nat Med. 2025 Jan 7. doi: 10.1038/s41591-024-03407-7.

Age-related TFEB downregulation in proximal tubules causes systemic metabolic disorders and occasional apolipoprotein A4-related amyloidosis
Nakamura J, Yamamoto T, Takabatake Y, Namba-Hamano T, Takahashi A, Matsuda J, Minami S, Sakai S, Yonishi H, Maeda S, Matsui S, Kawai H, Matsui I, Yamamuro T, Edahiro R, Takashima S, Takasawa A, Okada Y, Yoshimori T, Ballabio A, Isaka 
JCI Insight. 2024 Dec 19:e184451. doi: 10.1172/jci.insight.184451.

Spatiotemporal functions of leukemia inhibitory factor in embryo attachment and implantation chamber formation
Aikawa S, Hiraoka T, Matsuo M, Fukui Y, Fujita H, Saito-Fujita T, Shimizu-Hirota R, Takeda N, Hiratsuka D, He X, Ishizawa C, Iida R, Akaeda S, Harada M, Wada-Hiraike O, Ikawa M, Osuga Y, Hirota Y
Cell Death Discov. 2024 Nov 25;10(1):481. doi: 10.1038/s41420-024-02228-4.

Plant Research

Polyacetylenes and sesquiterpenes in Chinese traditional herb Atractylodes lancea: biomarkers and synergistic effects in red secretory cavities
Jiang D, Lin H, Liu Z, Qi K, Zhang W, Wang H, Zhang C, Zhu L, Zhu J, Zhang Y, Huang L, Wang S, Pan Y, Guo L
Mol Hortic. 2025 Feb 4;5(1):11. doi: 10.1186/s43897-024-00130-2.

RNA Extraction from Rice Immature Embryo Using Laser Capture Microdissection
Tezuka T, Nosaka-Takahashi M, Yoshikawa T, Sato Y
Methods in Molecular Biology, vol 2869. (2025) in: Kawakatsu, T. (eds) Rice.

Dissecting the cellular architecture and genetic circuitry of the soybean seed
Pelletier JM, Chen M, Lin J-Y, Le B, Kirkbride RC, Hur J, Wang T, Chang S-H, Olsona A, Nikolov L, Goldberg RB, and Harada JJ
PNAS, 30 dec 2024, Vol. 122, No. 1, e2416987121 

Forensics

Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Miscellaneous

Development of cyclopeptide inhibitors specifically disrupting FXR-coactivator interaction in the intestine as a novel therapeutic strategy for MASH
Li Y, Jiao T, Cheng X, Liu L, Zhang M, Li J, Wang J, Hu S, Li C, Yu T, Liu Y, Li Y, Zhang Y, Sun C, Sun J, Wang J, Xie C, Liu H
Life Metabolism, loaf004

Aβ40 Fibril Assembly on Human Cerebral Smooth Muscle Cells Impairs Cell Viability
Irizarry B, Davis J, Rajpoot J, Zhu X, Xu F, Smith SO, Van Nostrand WE
Biochemistry. 2025 Jan 6. doi: 10.1021/acs.biochem.4c00478.

Methods for Selective Gene Expression Profiling in Single Tertiary Lymphoid Structure Using Laser Capture Microdissection
Gutierrez-Chavez, C., Knockaert, S., Dieu-Nosjean, MC., Goc, J.
Dieu-Nosjean, MC. (eds) Tertiary Lymphoid Structures. Methods in Molecular Biology, vol 2864. Humana, New York, NY (2025). 

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Highlight:
Multiple: Laser Microdissection Protocols for Tissue and Cell Isolation
Bio-protocol Selections | 2024
https://en.bio-protocol.org/ebookinfo.aspx?id=23

Live Cell Culture

A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX

A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023

SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416