LAS X Life Science Microscope Software Platform

One for all

Supported Hardware (Widefield systems)	Short description
Leica microscope cameras	DFC7000 T, DFC7000 GT, K8, K5, K5C, K3C, K3M, DFC9000 GT, DFC9000 GTC, DFC3000 G, DMC2900, DMC4500, DMC5400, C1, C3, DMC6200, DFC450 C, DFC295, IC90E, ICC50W, ICC50E
Andor microscope cameras	iXonUltra 888, iXonUltra Life 897, iXonUltra 897
Hamamatsu microscope cameras	Flash 4.0 V2 CL, Flash 4.0 V2 USB, Flash 4.0 V3 CL, Flash 4.0 V3 USB
Photometrics microscope cameras	Prime 95B PCIe, Prime 95B USB3
Fluorescence light sources	LED8, LED5, LED3, LED1, EL6000, Lumen Dynamics X-Cite 200 DC, Lumen Dynamics X-Cite XLED1, CoolLED pE-800, CoolLED pE-300 White, CoolLED pE-300 Light, CoolLED pE340 Fura
Leica External Filter Wheels	High speed filter wheels including (27ms switching time)
Leica Water Immersion Micro Dispenser	Water immersion dispenser to compensate evaporation. Ideal for screening and multi positioning experiments (compatible objectives)
Leica MotCorr	Motorized movement of correction ring (dedicated objectives only)

Module name	Description	Widefield	Confocal
LAS X Core module	LAS X Core software, operates without the need for a dongle.	x	x
Multi Channel Acquisition	Allows the definition of up to 8 acquisition channels per experiment.	o	x
Time-Lapse	Define the duration and frequency of image capture for time lapse experiments.	o	x
Z-Control and Software Autofocus	Focus positioning and acquisition of 3 dimensional data sets. Includes software autofocus.	o	x
Mark and Find	Define multiple stage locations and revisit them as part of a time-lapse or Z stacking experiment.	o	x
Stitching	Create multiple regions of any shape for overview images and high resolution scans	o	x
Assay Editor	Perform multi well and multi chamber experiments. Create customized sample carriers. Automatically distribute regions and positions to each well/chamber.	o	o
Driver Non-Leica Cameras	Allows the control of any third party camera supported by LAS X.	o	o
Extended Depth of Field	Effectively sums up only the in focus area of each image in a three dimensional image stack and creates a single EDOF image.	o	-

x=included; o=optional; -=not available

Module name	Description	Widefield	Confocal
Calcium Imaging	Module for online ratio measurement, online display of ratio graphs and ratio image	o	x
Live Data Mode	Interactive data recording allowing job-sequencing and online evaluation	o	o
Colocalization	Histogram based colocalization and area measurements	o	o
FRET SE	Powerful wizard for FRET acquisition and analysis	o	o
Environmental Control	Have full control of your experimental conditions with the LAS X Environmental Control module. Log the environmental data and monitor it during the experiment.	o	o
User management	Allows system administrators to give different access levels to different LAS X user groups.	o	x
FRAP Wizard	The step by step wizard guides users through setup, acquisition and quantification of a FRAP experiment	o	o
HCS A	Versatile tool set for high content screening and automated microscopy.	o	o
Trigger to Peripherals	Send triggers and analog signals to peripherals. Incorporate triggers into your time lapse experiments. Includes powerful trigger capabilities such as sending a trigger at a specific channel or position.	o	-

x=included; o=optional; -=not available

Description	Widefield	Confocal
Graphic card based processing for smooth and fast motion of 3D volumes in real time	o	o
Wizard for 3D analysis	o	o
Easy and Versatile Wizard for 2D Analysis of Multi-Dimensional Data Sets	o	o
Fully integrated deconvolution algorithm using adaptive calculated or supplied PSF	o	o
Algorithms for no neighbor and nearest neighbor deconvolution	o	o
Multi-color restoration, channel unmixing	o	o
Digital overlays displayed on top of the camera image emulating fixed eyepiece graticules.	o	-

x=included; o=optional; -=not available

Module name	Description	Widefield	Confocal
Dye Assistant	Confocal users can select different dyes and LAS X sets up all hardware components accordingly.	-	x
Lambda Scan	Imaging method that acquires the emission spectrum in each image pixel using spectral detectors.	-	x
Lambda lambda scan	Imaging method that acquires the full excitation-emission spectrum in each image pixel using a tunable laser and spectral detectors. Allows Lambda square fluorescence mapping.	-	o
z-intensity compensation	Tool to compensate within a z-stack drop of fluorescence intensity that occurs deeper inside your sample with laser power and/or detector gain.	-	x
LightGate	Restriction of detection to a certain time window removes unwanted signal from autofluorescence or backscattering with HyD detectors.	-	o
FLIM	Wizard for fast and reliable setup of FLIM experiments and full control of FLIM data acquisition.	-	o
FCS	Wizard for fast and reliable setup of FCS and FLCS experiments and full control of F(L)CS data acquisition.	-	o
Electrophysiology	Package to guide you through E-Phys experiments, incl. Automated recordings, interactive data acquisition and trigger functions.	-	o
CRS Calculator	Automatic calculation of specific Raman wavenumbers according to the pumplaser wavelength used for exaction and vice versa.	-	o
CRS Spectral Scan	Automatic spectral scan covering the Raman wavenumbers range 1200-3400cm^-1	-	o

x=included; o=optional; -=not available

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