Spheroid Growth over 2.5 days
Mount sample
Mount sample
Position sample
Position sample
Define illumination 1
Start Live Acquisition
Define illumination 2
Define positions
Define illumination 3
Acquire
Define illumination 4
Choose images
Define detector gain
Run segmentation
Define pinhole
Run analysis
Define scanning mode
Define dwell time
Define offset
Define line step
Define averaging mode
Define averaging method
Define averaging number
Acquire
Process data
Choose images
Define thresholds
Define separators
Run segmentation
Run analysis
Extract information
Create figures
Sequential acquisition with a conventional microscope
Simultaneous acquisition with Mica