THUNDER Imager Live Cell & 3D Assay
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THUNDER Imager Live Cell & 3D Assay
Decode 3D biology in real time*
The following configurations were used in the applications provided in the examples.
THUNDER Imagers can be configured to your application. Get in contact with us.
THUNDER Imager | |||
3D Assay | Live Cell | Live Cell | |
Specimen | Retina | Brain organoid | Explant aorta |
Imaging Method | THUNDER | THUNDER | THUNDER |
Workflow | Navigator spiral scan | Navigator large tile scan | Long-term live cell culture |
Stand | DMi8 | DMi8 | DMi8 |
Focus | Z-Focus, motorized, closed-loop | THUNDER AFC (hardware focus for fast focusing and drift correction) | Motorized, closed-loop, AFC (hardware focus for fast focusing and drift correction) |
Stage | Scanning stage | QUANTUM high speed stage (real-time controlled with Synapse) | QUANTUM high speed stage (real-time controlled with Synapse) |
Transmitted light contrast & method | BF, DIC | BF | BF |
EFW | - | + | + |
Filter cubes | Single band pass | Quad | Quad |
Fluorescence illumination | LED3 | LED5 | LED8 |
Incubation | - | Stage-top incubation | Incubator i8, Temp, CO2 control |
Detection | K5 | DFC9000 GTC | DFC9000 GTC, Hamamatsu Flash4 |
Optional Upgrade path for advanced imaging workflows | - | + | + |
Optional Photomanipulation | - | Infinity Scanner | Infinity Scanner |
Optional TIRF | - | TIRF | TIRF |
Core strengths of our THUNDER Imagers:
THUNDER Imager | |||
Applications | 3D Assay | Live Cell | |
Monocell layers | ++ | +++ | |
Organoids or Spheroids | ++ | +++ | |
Zebrafish or C.Elegans, and other model organisms | ++ | +++ | |
Whole organs i.e. bio-printed or uncleared tissue of Mos | ++ | +++ | |
Thick tissue sections, i.e. biopsies or vibratome sections | ++ | +++ | |
Carriers | |||
Multi-well plates | +++ | +++ | |
Chamber slides | ++ | +++ | |
Glass slides | ++ | +++ | |
Petri dishes | + | +++ | |
Techniques | |||
Live cell imaging (Time lapse) | + | +++ | |
Contrast Methods (PhaCo, DIC, IMC) | +++ | ++ | |
TIRF | - | +++ | |
Photomanipulation | - | +++ | |
Ablation, i.e. FRAP/FRET | - | +++ |