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Bem-vindo ao portal de conhecimento da Leica Microsystems. Você encontrará pesquisas científicas e material didático sobre o tema microscopia. O portal oferece suporte a iniciantes, profissionais experientes e cientistas em seus trabalhos e experimentos diários. Explore tutoriais interativos e notas de aplicação, descubra os fundamentos da microscopia, bem como as tecnologias de ponta. Faça parte da comunidade do Science Lab e compartilhe sua experiência.
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Microscope Calibration for Measurements: Why and How You Should Do It
Microscope calibration ensures accurate and consistent measurements for inspection, quality control (QC), failure analysis, and research and development (R&D). Calibration steps are described in this…
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Depth of Field in Microscope Images
For microscopy imaging, depth of field is an important parameter when needing sharp images of sample areas with structures having significant changes in depth. In practice, depth of field is…
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What is Empty Magnification and How can Users Avoid it
The phenomenon of “empty magnification”, which can occur while using an optical, light, or digital microscope, and how it can be avoided is explained in this article. The performance of an optical…
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The Guide to STED Sample Preparation
This guide is intended to help users optimize sample preparation for stimulated emission depletion (STED) nanoscopy, specifically when using the STED microscope from Leica Microsystems. It gives an…
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How to Prepare Samples for Stimulated Raman Scattering (SRS) imaging
Find here guidelines for how to prepare samples for stimulated Raman scattering (SRS), acquire images, analyze data, and develop suitable workflows. SRS spectroscopic imaging is also known as SRS…
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Understanding Clearly the Magnification of Microscopy
To help users better understand the magnification of microscopy and how to determine the useful range of magnification values for digital microscopes, this article provides helpful guidelines.
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Introduction to Fluorescent Proteins
Overview of fluorescent proteins (FPs) from, red (RFP) to green (GFP) and blue (BFP), with a table showing their relevant spectral characteristics.
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How Marine Microorganism Analysis can be Improved with High-pressure Freezing
In this application example we showcase the use of EM-Sample preparation with high pressure freezing, freeze substiturion and ultramicrotomy for marine biology focusing on ultrastructural analysis of…
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What is the FusionOptics Technology?
Leica stereo microscopes with FusionOptics provide optimal 3D perception. The brain merges two images, one with large depth of field and the other with high resolution, into one 3D image.