James DeRose , Ph.D.
James DeRose is a Global Marketing Communication Manager at Leica Microsystems. His focus is on optimized content creation for applications concerning life science, material science, as well as industry and manufacturing. He is involved with application notes, case studies, technical reports, white papers, application pages, product pages, social media posts, emails, testimonials, and other materials. He has worked at Leica Microsystems since 2013. In the past, he worked on applications development projects in the fields of interfacial chemistry and physics, thermal and chemical engineering, corrosion and metallography, surface coatings, materials science, biotechnology, and cellular biology. He has expertise with various types of microscopy and analytical methods.
Phase Contrast and Microscopy
This article explains phase contrast, an optical microscopy technique, which reveals fine details of unstained, transparent specimens that are difficult to see with common brightfield illumination.
Immersion Objectives
How an immersion objective, which has a liquid medium between it and the specimen being observed, helps increase the numerical aperture and microscope resolution is explained in this article.
How to Determine Cell Confluency with a Digital Microscope
This article shows how to measure cell confluency in an easy and consistent way with Mateo TL, increasing confidence in downstream experiments.
Microscope Resolution: Concepts, Factors and Calculation
This article explains in simple terms microscope resolution concepts, like the Airy disc, Abbe diffraction limit, Rayleigh criterion, and full width half max (FWHM). It also discusses the history.
What is FRET with FLIM (FLIM-FRET)?
This article explains the FLIM-FRET method which combines resonance energy transfer and fluorescence lifetime imaging to study protein-protein interactions.
3 Factors Determine the Damage Potential of Particles
This article discusses the 3 factors for determining the potential of a particle to cause damage to parts and components in the automotive and electronic industry. These factors include the…
Studying Ocular Birth Defects
This article discusses how lens formation and ocular birth defects can be studied with sharp widefield microscopy images which are acquired rapidly. The mouse ocular lens is used as a model to study…
Deep Visual Proteomics Provides Precise Spatial Proteomic Information
Despite the availability of imaging methods and mass spectroscopy for spatial proteomics, a key challenge that remains is correlating images with single-cell resolution to protein-abundance…
RNA Quality after Different Tissue Sample Preparation
The influence of sample preparation and ultraviolet (UV) laser microdissection (UV LMD) on the quality of RNA from murine-brain tissue cryo-sections is described in this article. To obtain good…
Factors to Consider for a Cleanliness Analysis Solution
Choosing the right cleanliness analysis solution is important for optimal quality control. This article discusses the important factors that should be taken into account to find the solution that best…
A Guide to Fluorescence Lifetime Imaging Microscopy (FLIM)
The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.
Cleanliness Analysis for Particulate Contamination
Devices, products, and their components fabricated in many industries can be quite sensitive to contamination and, as a result, have stringent requirements for technical cleanliness. Measurement…
Efficient Particle Counting and Analysis
This report discusses particle counting and analysis using optical microscopy for cleanliness of parts and components. Particle counting and analysis is a critical part of quality assurance in the…
Quality Control Under the Microscope
Fast-rising demand for electric vehicles is one of the market’s main drivers, but there are other hotspots of growth, including the rise in renewable energy installations, such as photovoltaic panels,…
Hydraulics in the Automotive and Aerospace Industries
Cleanliness standards relating to lubricants, hydraulic fluids, and oils, e.g., ISO 4406 and DIN 51455 are discussed in this article. Cleanliness plays a central role in the automotive and…
Studying Wound Healing of Smooth Muscle Cells
This article discusses how wound healing of cultured smooth muscle cells (SMCs) in multiwell plates can be reliably studied over time with less effort using a specially configured Leica inverted…
Cleanliness of Automotive Components and Parts
This article discusses the ISO 16232 standard and VDA 19 guidelines and briefly summarizes the particle analysis methods. They give important criteria for the cleanliness of automotive parts and…
Multi-Color Caspase 3/7 Assays with Mica
Caspases are involved in apoptosis and can be utilized to determine if cells are undergoing this programmed cell death pathway in so-called caspase assays. These assays can be run by e.g. flow…
Fast, High-contrast 3D Imaging of Sensory Neurons
This article discusses how fast, high-contrast 3D imaging of dorsal root ganglion (DRG) tissue with a THUNDER Imager Tissue using large volume computational clearing (LVCC) allows sensory neurons to…
Find Relevant Specimen Details from Overviews
Switch from searching image by image to seeing the full overview of samples quickly and identifying the important specimen details instantly with confocal microscopy. Use that knowledge to set up…
Verifying Specifications for Auto Parts and Components
During the development and production of automotive parts and components, whether by suppliers or the auto manufacturer, specifications must be met. This goal is important, because the specifications…
Top Challenges for Visual Inspection
This article discusses the challenges encountered when performing visual inspection and rework using a microscope. Using the right type of microscope and optical setup is paramount in order to…
How to Select the Right Solution for Visual Inspection
This article helps users with the decision-making process when selecting a microscope as a solution for routine visual inspection. Important factors that should be considered are described.
Introduction to 21 CFR Part 11 and Related Regulations
This article provides an overview of regulations and guidelines for electronic records (data entry, storage, signatures, and approvals) used in the USA (21 CFR Part 11), EU (GMP Annex 11), and China…
Why is Manual Visual Inspection of Medical Devices so Challenging?
This article discusses how manual visual inspection, which is prevalent in the medical device industry, can lead to inconsistent results. It also addresses the challenges quality managers and…
Studying Cell Division
Cell division is a biological process during which all cellular components must be distributed among the daughter cells. The division process requires firm coordination for success. Microscopy is…
Investigating Synapses in Brain Slices with Enhanced Functional Electron Microscopy
A fundamental question of neuroscience is: what is the relationship between structural and functional properties of synapses? Over the last few decades, electrophysiology has shed light on synaptic…
Topographic Analysis of Firing Pin Impressions on Cartridge Cases
The analysis of fired cartridges for primer cup morphology and flattening and firing pin impression (crater) depth using topographic data is discussed in this article. Topographical analysis of the…
Studying Pulmonary Fibrosis
The results shown in this article demonstrate that fibrotic and non-fibrotic regions of collagen present in mouse lung tissue can be distinguished better with polarized light compared to brightfield.…
Keeping Particulate Contamination Under Control in Pharmaceutical Products
This article describes how a 2-methods-in-1 solution combining optical microscopy and laser induced breakdown spectroscopy (LIBS) can be utilized for identification of particulate contaminants in the…
