Jan De Bock , Dr.
Application Manager (Life Science Research), Leica Microsystems CMS GmbH.
Jan has worked as a microscope professional in different roles since 2003. He joined Leica Microsystems in 2011 as a product specialist for confocal microscopy. In 2017, he became a member of the application management team where he is also responsible for correlative workflows, involving microscope systems under cryogenic conditions and sample preparation equipment. Jan studied biology and earned his PhD in the field of olfactory research.
How to Automatically Obtain Fluorescent Cells of Interest in a Block-face
Block-face created by automatic trimming under fluorescence.
Mammalian cells of interest, stained with CellTrackerTM Green are visualized within the block-face using the UC Enuity equipped with the…
Automatic Alignment of Sample and Knife for High Sectioning Quality
Automatic alignment of sample and knife on the ultramicrotome UC Enuity, enabling even untrained users to create ultrathin sections with reduced risk of losing precious sections.
New Imaging Tools for Cryo-Light Microscopy
New cryo-light microscopy techniques like LIGHTNING and TauSense fluorescence lifetime-based tools reveal structures for cryo-electron microscopy.
How to Target Fluorescent Structures in 3D for Cryo-FIB Milling
This article describes the major steps of the cryo-electron tomography workflow including super-resolution cryo-confocal microscopy. We describe how subcellular structures can be precisely located in…
How to Improve Live Cell Imaging with Coral Life
For live-cell CLEM applications, light microscopy imaging is a critical step for identifying the right cell in the right state at the right time. In this article, Leica experts share their insights on…
Targeting Active Recycling Nuclear Pore Complexes using Cryo Confocal Microscopy
In this article, how cryo light microscopy and, in particular cryo confocal microscopy, is used to improve the reliability of cryo EM workflows is described. The quality of the EM grids and samples is…
Crystal Clear Cryo Light-microscopy Images
This article describes how computational clearing of cryo light microscopy images improves the identification of cellular targets for cryo electron-microscopy.
Mosaic Images
Confocal laser scanning microscopes are widely used to create highly resolved 3D images of cells, subcellular structures and even single molecules. Still, an increasing number of scientists are…
