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Cell Biology Image Gallery

Cell biology images taken with Leica Microsystems instruments

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Cell biology studies the structure, function and behavior of cells, including cell metabolism, cell cycle, and cell signaling. Fluorescence microscopes are an integral part of a cell biologist toolkit. Widefield and confocal microscopes are used widely to visualize the details of complex structures in the cellular context.

Cell biology imaging with STELLARIS confocal platform

The STELLARIS family of confocal microscopes provides you with high-end solutions that enable the study of live cell specimens, detailed single molecule analysis or helps you maximize the results you get out of your experiments.

Volumetric image of a Zebrafish beating heart

Heart muscle cells (GFP) allow to follow the heartbeat dynamics. The transmission allows to observe the corresponding blood flow. Video acquired with DLS light sheet imaging, with fluorescence and transmission.

Transcription activity in mice gastric stem cell derived organoid

Hoechst channel labelling the nucleus. The differences in fluorescence lifetime corresponds to difference in DNA compaction. The longer lifetimes (red) correspond to cells with more open reading frames and an active DNA transcription such as stem cells. Video acquired with multiphoton (DIVE) and lifetime (FALCON).

Gentle 3D live imaging of Hydractinia symbiolongicarpus

Hydractinia expressing eGFP under the control of neurogenesis-related genes label. Imaged with DLS and LIGTHNING.

Zebrafish heart muscle cells and blood flow in transmission

Heart muscle cells (GFP) allow to follow the heartbeat dynamics. The transmission allows to observe the corresponding blood flow. Video acquired with DLS light sheet imaging, with fluorescence and transmission.

Live NE-115 cells expressing mNeonGreen-LifeAct

Live NE-115 cells expressing mNeonGreen-LifeAct are stained with MitoTracker Green, NucRed and SiR Tubulin.

Live NE-115 cells expressing mNeonGreen-LifeAct are stained with MitoTracker Green, NucRed and SiR Tubulin. Signals are acquired with 2 detectors for two fluorophores each. Intensity image shows in yellow and gray. Signal in each detector will be distingu

Maximum projection of a z-stack from fixed U2OS cells labelled with 5 color

LIGHTNING image overlay shows the maximum projection of a z-stack from fixed U2OS cells labelled with 5 color: AF488 (Tubulin, grey), SPY555 (Actin, magenta), MitoTrackerRed (Lumen of Mitochondria, green), Atto 647N (TOM 20, Mitochondria, red), CF770 WGA (Membranes, cyan).​​ scale bar 10µm

Cytoskeleton and membranes in live cell imaged with TauSTED

Image acquired with STELLARIS STED.

Cytoskeleton and membranes in live cell imaged with TauSTED - Confocal Cytoskeleton and membranes in live cell imaged with TauSTED - STED

TauSTED 775 resolves the intricate cytoskeleton network labeled with SiR-tubulin (glow - Spyrochrome), and trafficking vesicles labeled with CF594 (cyan - Biotium).

3D live Imaging of Spirogyra sp.

3D live Imaging of Spirogyra sp. Image acquired with DLS and LIGHTNING.
Endogeneous fluorescence of chloroplasts in green algea Spirogyra sp. Xyz-stack 3D-animation with STELLARIS 3D-viewer depth color coding.

Exploring Chloroplast activity with multiphoton and fluorescence lifetime imaging

Image acquired with a STELLARIS 8 DIVE FALCON.

The multiphoton transmission image shows the leaf structure, the endogenous chloroplast fluorescence is shown in green. Then the Fluorescence lifetime imaging fingerprint from a phasor analysis converts this endogenous to colors depending on the oxidative potential for each chloroplast.

Endocytic pathway and mitochondria dynamics

Mitochondria labelled with MitoView Green. Membrane vesicles labelled with CellBright NIR750. The distinct endocytic steps are revealed with TauSeparation with lifetime component separation based on pH maturation steps correlated to endocytic maturation of vesicles

Cytoskeleton and membranes in live cell imaged with TauSTED

Image acquired with STELLARIS STED. Live cell TauSTED on U2OS cells, using labels for actin (SiR-actin, glow), microtubules (SPY555-tubulin, cyan), and membranes (CF488A coupled to WGA, green). SiR and SPY are available from Spirochrome. CF dyes are available from Biotium Inc. Scale bar: 10 μm

Live cell TauSTED on U2OS cells - confocal Live cell TauSTED on U2OS cells - sted

Cytoskeleton and membranes in live cell imaged with TauSTED

3D confocal image of C. elegans neurons labelled with four red fluorescent proteins.

Image acquired with STELLARIS 8 FALCON with a single excitation line and a single detector. The 4 distinct signals were obtained with phasor separation. The use of a single laser line and single detector improves live imaging speed and lowers the laser dose. Grey: Intensity only. Colors: Merged phasor-separated signals. Scale bar: 20 μm. Tracking 4 neuronal types with 4 red fluorescent proteins on a single detector with FLIM.

3D confocal image of C. elegans neurons 3D confocal image of C. elegans neurons

3D confocal image of C. elegans neurons labelled with four red fluorescent proteins

Mitochondria-localized GFP in Magnaporthe Oryzae (Rice blast fungus)

Mitochondria-localized GFP in Magnaporthe Oryzae (Rice blast fungus)
TauContrast distinguishes same fluorescent protein in different cell compartments in fungi. Mitochondria-localized GFP in Magnaporthe Oryzae (Rice blast fungus). As a new cell forms (to the right), the older cells die and the GFP starts to be seen in the vacuole. We can see this in the GFP channel (left) but we also seen a nice TauContrast change with the AAT (average arriving time) being longer for GFP in the vacuole compared to the mitochondria. Sample provided by Shen Qing, Naweed Naqvi lab, Temasek Lifescience Laboratory, Singapore.

Kita mycelia expressing mCherry at the membrane and in the interior​

L form streptomyces
Kita mycelia with mCherry expressed at the membrane and in the interior of the bacteria. mCherry can be separated using the TauSeparation tool in Tausense, as it exhibits different lifetime components depending in where it is localized. Sample courtesy: Shraddha Shitut, Insitute of Biology, Leiden University.​

STED for malaria research

STED for malaria research
STED for malaria research: STED nanoscopy of merozoites invading erythrocytes. 3D STED image showing overlays of RON4 (magenta) with the proteins PfRh5 (left, green), PfRipr (middle, green), PfCyRPA (right, green). Nuclear staining with DAPI is shown in blue, and erythrocytic membrane is shown in gray. Scale bar: 1 µm. Image courtesy: Jennifer Volz, Alan Cowman, Walter and Eliza Hall Institute of Medical Research, Australia, and Marko Lampe, EMBL, Germany.

Three color STED imaging of Cos 7 cells

Three color STED imaging of Cos 7 cells
Three color STED imaging of Cos 7 cells stained with antibodies against NUP153 (green), TOM20 (magenta), and with SiR–actin (gray). Confocal (top) and STED (bottom). Scale bar: 2μm. Sample courtesy: Urs Ziegler and Jana Doehner, ZMB, University of Zurich, Switzerland.

Living T cell in suspension

Living T cell in suspension. 3D reconstruction of confocal and STED stacks.
3D reconstruction of confocal and STED stacks. Image courtesy: Marco Fritsche, Mathias Clausen and Christian Eggeling, MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, UK.

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