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Leica Microsystems

Leica Microsystems

Leica Microsystems is a world leader in microscopes and scientific instruments. Founded as a family business in the nineteenth century, the company’s history was marked by unparalleled innovation on its way to becoming a global enterprise.

Its historically close cooperation with the scientific community is the key to Leica Microsystems’ tradition of innovation, which draws on users’ ideas and creates solutions tailored to their requirements. At the global level, Leica Microsystems is organized in three divisions, all of which are among the leaders in their respective fields: Life Science, Industry and Medical.

The company is represented in over 100 countries with 6 manufacturing facilities in 5 countries, sales and service organizations in 20 countries, and an international network of dealers. The company is headquartered in Wetzlar, Germany.

http://www.leica-microsystems.com/

Molecular structure of the green fluorescent protein (GFP)

Introduction to Fluorescent Proteins

Overview of fluorescent proteins (FPs) from, red (RFP) to green (GFP) and blue (BFP), with a table showing their relevant spectral characteristics.
Caption: Collaboration is key Picture copyright: Donenko Oleksii, Shutterstock

Launching a Neurosurgical Department with Limited Resources

Learn about Dr. Claire Karekezi’s journey and experience launching a neurosurgical department within the Rwanda Military Hospital with limited resources.

Coherent Raman Scattering Microscopy Publication List

CRS (Coherent Raman Scattering) microscopy is an umbrella term for label-free methods that image biological structures by exploiting the characteristic, intrinsic vibrational contrast of their…
Image of an onion flake taken with a basic Leica compound microscope after it was tested for resistance to fungus and mold growth following part 11 of the ISO 9022 standard.

ISO 9022 Standard Part 11 - Testing Microscopes with Severe Conditions

This article describes a test to determine the robustness of Leica microscopes to mold and fungus growth. The test follows the specifications of the ISO 9022 part 11 standard for optical instruments.
Image of an integrated-circuit (IC) chip cross section acquired at higher magnification showing a region of interest.

Structural and Chemical Analysis of IC-Chip Cross Sections

This article shows how electronic IC-chip cross sections can be efficiently and reliably prepared and then analyzed, both visually and chemically at the microscale, with the EM TXP and DM6 M LIBS
EBSD grain size distribution of the cross section of a gold wire within a silicon matrix from inside a CPU (central processing unit of a computer). The grains are highlighted with arbitrary colors.

High-Quality EBSD Sample Preparation

This article describes a method for EBSD sample preparation of challenging materials. The high-quality samples required for electron backscatter diffraction are prepared with broad ion-beam milling.
Augmented Reality fluorescence supports each step of neurovascular surgery procedures. Image courtesy of Dr. Christof Renner.

Use of AR Fluorescence in Neurovascular Surgery

Learn about the use of GLOW800 Augmented Reality in neurovascular surgery through clinical cases and videos, including aneurysm and tumor resection cases.
C. elegans adult hermaphrodite gonades acquired using THUNDER Imager. Staining: blue - DAPI (nucleus), green - SP56 (sperm), red - RME-2 (oocyte), magenta - PGL-1 (RNA + protein granules). Image courtesy of Prof. Dr. Christian Eckmann, Martin Luther University, Halle, Germany.

Life Science Research: Which Microscope Camera is Right for You?

Deciding which microscope camera best fits your experimental needs can be daunting. This guide presents the key factors to consider when selecting the right camera for your life science research.
Pancreatic Ductal Adenocarcinoma with 11 Apoptosis biomarkers shown – BAK, BAX, BCL2, BCLXL, Caspase9, CIAP1, NaKATPase, PCK26, SMAC, Vimentin, and XIAP.

Multiplexing with Luke Gammon: Advance your Spatial Biology Research

Learn how multiplexing imaging and spatial biology can help researchers better understand complex biological systems. In this interview, Dr. Gammon and Dr. Pointu of Leica Microsystems discuss pain…
Multi-tissue array with 4 markers shown including DAPI, NaKATPase, PanCk, and Vimentin.

Spatial Biology: Learning the Landscape

Spatial Biology: Understanding the organization and interaction of molecules, cells, and tissues in their native spatial context
Stereo microscopes are often considered the workhorses of laboratories and production sites.

Key Factors to Consider When Selecting a Stereo Microscope

This article explains key factors that help users determine which stereo microscope solution can best meet their needs, depending on the application.
Brain organoid section (DAPI) acquired using THUNDER Imager Live Cell. Image courtesy of Janina Kaspar and Irene Santisteban, Schäfer Lab, TUM.

Imaging Organoid Models to Investigate Brain Health

Imaging human brain organoid models to study the phenotypes of specialized brain cells called microglia, and the potential applications of these organoid models in health and disease.

Rapid and Reliable Examination of PCBs & PCBAs with Digital Microscopy

Digital microscopes provide users with a convenient and rapid way to acquire high-quality, reliable image data and make quick inspection and analysis of printed circuit boards (PCBs) and assemblies…
In vivo imaging of a mouse pial and cortical vasculature through a glass window (ROSAmT/mG::Pdgfb-CreERT2 mouse meningeal and cortical visualization following tamoxifen induction and craniotomy). Courtesy: Thomas Mathivet, PhD

Windows on Neurovascular Pathologies

Discover how innate immunity can sustain deleterious effects following neurovascular pathologies and the technological developments enabling longitudinal studies into these events.
The principle of the FusionOptics technology:  Of the two separate beam paths (1), one provides depth of field (2) and the other high resolution (3). In the brain, the two images of the sample are merged into a single, optimal 3D image (4).

What is the FusionOptics Technology?

Leica stereo microscopes with FusionOptics provide optimal 3D perception. The brain merges two images, one with large depth of field and the other with high resolution, into one 3D image.
Lifetime-based multiplexing in live cells using TauSeparation. Mammalian cells expressing LifeAct-GFP (ibidi GmbH) and labelled with MitoTracker Green. Acquisition with one detector, intensity information shown in grey. The two markers can be separated using lifetime information: LifeAct-GFP (cyan), MitoTracker Green (magenta). Image acquired with STELLARIS 5.

The Power of Reproducibility, Collaboration and New Imaging Technologies

In this webinar you willl learn what impacts reproducibility in microscopy, what resources and initiatives there are to improve education and rigor and reproducibility in microscopy and how…
Branched organoid growing in collagen where the Nuclei are labeled blue. To detect the mechanosignaling process, the YAP1 is labeled green.

Examining Developmental Processes In Cancer Organoids

Interview: Prof. Bausch and Dr. Pastucha, Technical University of Munich, discuss using microscopy to study development of organoids, stem cells, and other relevant disease models for biomedical…
Mouse cortical neurons. Transgenic GFP (green). Image courtesy of Prof. Hui Guo, School of Life Sciences, Central South University, China

How Microscopy Helps the Study of Mechanoceptive and Synaptic Pathways

In this podcast, Dr Langenhan explains how microscopy helps his team to study mechanoceptive and synaptic pathways, their challenges, and how they overcome them.
Advanced technologies support neurosurgical teaching. Image courtesy of Dr. Florian Bernard.

3D, AR & VR for Teaching in Neurosurgery

Discover the evolution of neurosurgical teaching and how 3D, Augmented Reality and Virtual Reality can help better learn anatomy and acquire surgical skills.
Cell counts for each biomarker were divided by total number of cells to give a percentage of biomarker positive cells out of total cells for each biomarker.

Methods to Improve Reproducibility in Spatial Biology Research

Establish reproducibility results for a Cell DIVE multiplexed imaging study in cancer research using the BAB 200 automated system from ASLS and validated antibodies from CST
THY1-EGFP labeled neurons in mouse brain processed using the PEGASOS 2 tissue clearing method, imaged on a Leica confocal microscope. Neurons were traced using Aivia’s 3D Neuron Analysis – FL recipe. Image credit: Hu Zhao, Chinese Institute for Brain Research.

Unlocking Insights in Complex and Dense Neuron Images Guided by AI

The latest advancement in Aivia AI image analysis software provides improved soma detection, additional flexibility in neuron tracing, 3D relational measurement including Sholl analysis and more.
PDAC Multiplexed imaging of CST panels enables an examination of immune cell components in pancreatic ductal adenocarcinoma (IPDAC) tissue on a single slide.

Characterizing tumor environment to reveal insights and spatial resolution

Antibodies from Cell Signaling Technology are validated for use with the Cell DIVE multiplexing workflow and used to probe cell lineages in the tumor microenvironment
Microscopy for neuroscience research

What are the Challenges in Neuroscience Microscopy?

eBook outlining the visualization of the nervous system using different types of microscopy techniques and methods to address questions in neuroscience.
AI-based workflow for fast rare event detection in living biological samples using Autonomous Microscopy powered by Aivia

AI Microscopy Enables the Efficient Detection of Rare Events

Localization and selective imaging of rare events is key for the investigation of many processes in biological samples. Yet, due to time constraints and complexity, some experiments are not feasible…
Fluorescence microscope image of a life-science specimen

An Introduction to Fluorescence

This article gives an introduction to fluorescence and photoluminescence, which includes phosphorescence, explains the basic theory behind them, and how fluorescence is used for microscopy.

How to Prepare and Analyse Battery Samples with Electron Microscopy

This workshop covers the sample preparation process for lithium and novel battery sample analysis, as well as other semiconductor samples requiring high-resolution cross-section imaging.
Cancer cells

The Role of Iron Metabolism in Cancer Progression

Iron metabolism plays a role in cancer development and progression, and modulates the immune response. Understanding how iron influences cancer and the immune system can aid the development of new…
Pancreatic Ductal Adenocarcinoma with 5 biomarkers shown – SMA, PanCK PCK26, PanCK AE1, Vimentin, and Glut1.

Dig Deeper Into the Complexities of Pancreatic Cancer with Multiplex Imaging

Cell DIVE is an iterative staining workflow for multiplexed imaging that unveils biological pathways to dig deeper into the complexities of pancreatic cancer.
Raw widefield and THUNDER image of calcium transients in Drosophila embryos. Courtesy A. Carreira-Rosario, Clandinin laboratory, California, USA.

Central Nervous System (CNS) Development and Activity in Organisms

This article shows how studying central nervous system (CNS) development in Drosophila-melanogaster embryos expressing a GCaMP calcium indicator in the neurons can be improved with a THUNDER Imager.
How is microscopy used in spatial biology - Teaserimage

How is Microscopy Used in Spatial Biology? A Microscopy Guide

Different spatial biology methods in microscopy, such as multiplex imaging, are helping to better understand tissue landscapes. Learn more in this microscopy guide.
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