Leica Microsystems

Leica Microsystems

Leica Microsystems is a world leader in microscopes and scientific instruments. Founded as a family business in the nineteenth century, the company’s history was marked by unparalleled innovation on its way to becoming a global enterprise.

Its historically close cooperation with the scientific community is the key to Leica Microsystems’ tradition of innovation, which draws on users’ ideas and creates solutions tailored to their requirements. At the global level, Leica Microsystems is organized in three divisions, all of which are among the leaders in their respective fields: Life Science, Industry and Medical.

The company is represented in over 100 countries with 6 manufacturing facilities in 5 countries, sales and service organizations in 20 countries, and an international network of dealers. The company is headquartered in Wetzlar, Germany.

http://www.leica-microsystems.com/

Optical microscope image of salt contamination on an aluminum/silicon (Al/Si) surface. Credit: Gerweck GmbH, Germany.

Microscopic Defects in Electroplating

This free on-demand webinar shows how to identify quickly root causes of defects during electroplating of components with optical microscopy and LIBS (laser spectroscopy).

Insights into Vesicle Trafficking

STELLARIS provides integral access to complementary layers of information for dynamic, structural, and mechanistic insights into vesicle trafficking.

Visualizing Protein-Protein Interactions by Non-Fitting and Easy FRET-FLIM Approaches

The Webinar with Dr. Sergi Padilla-Parra is about visualizing protein-protein interaction. He gives insight into non-fitting and easy FRET-FLIM approaches.
Living HeLa cells stained with WGA-488 (yellow), SPY-Actin (cyan), and SiR-Tubulin (magenta). Instant Computational Clearing (ICC) was applied.

How to Perform Dynamic Multicolor Time-Lapse Imaging

Live-cell imaging sheds light on diverse cellular events. As many of these events have fast dynamics, the microscope imaging system must be fast enough to record every detail. One major advantage of…
Spectral separation of 11 fluorophores coupled to polystyrene beads on a STELLARIS confocal system.

Multiplexing through Spectral Separation of 11 Colors

Fluorescence microscopy is a fundamental tool for life science research that has evolved and matured together with the development of multicolor labeling strategies in cells tissues and model…

3 Factors Determine the Damage Potential of Particles

This article discusses the 3 factors for determining the potential of a particle to cause damage to parts and components in the automotive and electronic industry. These factors include the…
Raw widefield and THUNDER image of transversal mouse adult fiber lens section. Courtesy N. Houssin, Plagemen lab, Ohio State University, Columbus, USA.

Studying Ocular Birth Defects

This article discusses how lens formation and ocular birth defects can be studied with sharp widefield microscopy images which are acquired rapidly. The mouse ocular lens is used as a model to study…
Two-color caspase assay with tile scan. U2OS cells were treated with the nuclear marker DRAQ5 (magenta) and CellEvent™ (yellow).

Following Multiple Events during Staurosporine Apoptosis

In this video on demand, we show how adding additional markers to an apoptosis kit can markedly increase the amount of information a researcher can obtain from the same experiment. The simultaneous…
Image of murine-brain tissue showing a region removed with UV laser microdissection.

RNA Quality after Different Tissue Sample Preparation

The influence of sample preparation and ultraviolet (UV) laser microdissection (UV LMD) on the quality of RNA from murine-brain tissue cryo-sections is described in this article. To obtain good…
Analysis of an aneurysm with Virtual Reality. Image courtesy of Prof. Raphael Guzman.

Digitalization in Neurosurgical Planning and Procedures

Learn about Augmented Reality, Virtual Reality and Mixed Reality in neurosurgery and how they can help overcome challenges.
The various solutions from Leica Microsystems for cleanliness analysis.

Factors to Consider for a Cleanliness Analysis Solution

Choosing the right cleanliness analysis solution is important for optimal quality control. This article discusses the important factors that should be taken into account to find the solution that best…
Projection of a confocal z-stack. Sum159 cells, human breast cancer cells kindly provided by Ievgeniia Zagoriy, Mahamid Group, EMBL Heidelberg, Germany. Blue–Hoechst - indicates nuclei, Green–MitoTracker mitochondria, and red–Bodipy - lipid droplets

New Imaging Tools for Cryo-Light Microscopy

New cryo-light microscopy techniques like LIGHTNING and TauSense fluorescence lifetime-based tools reveal structures for cryo-electron microscopy.
Transverse histological cut of a rabbit tongue. 50 Mpixels images (2326 µm x 1739 µm) in 14 x 18 tiles. Lifetime gives an additional contrast that allows to differentiate different structures in histological stainings.

A Guide to Fluorescence Lifetime Imaging Microscopy (FLIM)

The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.
Staining is crucial to revealing details in the specimens.

H&E Staining in Microscopy

If we consider the role of microscopy in pathologists’ daily routines, we often think of the diagnosis. While microscopes indeed play a crucial role at this stage of the pathology lab workflow, they…
Untreated Hela Kyoto cells stained to show the nucleus (Hoechst, blue), the cis-golgi matrix protein GM130 (AF488, green), and the trans-golgi network membrane protein TGN46 (AF647, red).

Golgi Organizational Changes in Response to Cell Stress

In this video on demand, our special guest George Galea from EMBL Heidelberg will look at HeLa Kyoto cells treated with various chemotherapeutic agents to investigate their effect on the Golgi complex…
Type of contamination: spores

Cleanliness Analysis for Particulate Contamination

Devices, products, and their components fabricated in many industries can be quite sensitive to contamination and, as a result, have stringent requirements for technical cleanliness. Measurement…
Particles and fibers on a filter which will be counted and analyzed for cleanliness

Efficient Particle Counting and Analysis

This report discusses particle counting and analysis using optical microscopy for cleanliness of parts and components. Particle counting and analysis is a critical part of quality assurance in the…
Protist Paramecium (Paramecium tetraurelia) stained to show the nucleus

3D Spatial Analysis Using Mica's AI-Enabled Microscopy Software

This video offers practical advice on the extraction of publication grade insights from microscopy images. Our special guest Luciano Lucas (Leica Microsystems) will illustrate how Mica’s AI-enabled…
A stack of lithium-ion batteries

Quality Control Under the Microscope

Fast-rising demand for electric vehicles is one of the market’s main drivers, but there are other hotspots of growth, including the rise in renewable energy installations, such as photovoltaic panels,…
Pancreatic ductal adenocarcinoma tissue section imaged with Cell DIVE

Multiplexed Imaging Types, Benefits and Applications

Multiplexed imaging is an emerging and exciting way to extract information from human tissue samples by visualizing many more biomarkers than traditional microscopy. By observing many biomarkers…
3D Reconstruction of brain slide image_Mica

3D Tissue Imaging: From Fast Overview To High Resolution With One Click

3D Tissue imaging is a widespread discipline in the life sciences. Researchers use it to reveal detailed information of tissue composition and integrity, to make conclusions from experimental…
MDCK cysts on day 9

How To Perform Fast & Stable Multicolor Live-Cell Imaging

With the help of live-cell imaging researchers gain insights into dynamic processes of living cells up to whole organisms. This includes intracellular as well as intercellular activities. Protein or…
Image of a single slice taken from a zebrafish heart showing the ventricle with an injury in the lower area. Nuclei of all cells are indicated with blue, nuclei of the cardiomyocyte heart muscle cells with green, and the proliferating cells with red. Courtesy of Laura Peces-Barba Castaño, Max Planck Institute for Heart and Lung Research, Germany.

Imaging of Cardiac Tissue Regeneration in Zebrafish

Learn how to image cardiac tissue regeneration in zebrafish focusing on cell proliferation and response during recovery with Laura Peces-Barba Castaño from the Max Planck Institute.
When particulate contamination is present in lubricating fluids or oils, it can cause damage to parts or components leading to malfunctions.

Hydraulics in the Automotive and Aerospace Industries

Cleanliness standards relating to lubricants, hydraulic fluids, and oils, e.g., ISO 4406 and DIN 51455 are discussed in this article. Cleanliness plays a central role in the automotive and…

TauInteraction – Studying Molecular Interactions with TauSense

Fluorescence microscopy constitutes one of the pillars in life sciences and is a tool commonly used to unveil cellular structure and function. A key advantage of fluorescence microscopy resides in the…
Images of smooth muscle cells during wound healing. Courtesy L.S. Shankman, Ph.D., University of Virginia.

Studying Wound Healing of Smooth Muscle Cells

This article discusses how wound healing of cultured smooth muscle cells (SMCs) in multiwell plates can be reliably studied over time with less effort using a specially configured Leica inverted…
Particles which could be found during cleanliness analysis of parts and components.

Cleanliness of Automotive Components and Parts

This article discusses the ISO 16232 standard and VDA 19 guidelines and briefly summarizes the particle analysis methods. They give important criteria for the cleanliness of automotive parts and…

How Does The Cytoskeleton Transport Molecules?

VIDEO ON DEMAND - See how 3D cysts derived from MDCK cells help scientists understand how proteins are transported and recycled in tissues and the role of the cytoskeleton in this transport.
Developing zebrafish (Danio rerio) embryo, from sphere stage to somite stages.

Studying Early Phase Development of Zebrafish Embryos

This video on demand focuses on combining widefield and confocal imaging to study the early-stage development of zebrafish embryos (Danio rerio), from oocyte to multicellular stage.
Two-color caspase assay with tile scan

Multi-Color Caspase 3/7 Assays with Mica

Caspases are involved in apoptosis and can be utilized to determine if cells are undergoing this programmed cell death pathway in so-called caspase assays. These assays can be run by e.g. flow…
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