Life Science Research

This is the place to expand your knowledge, research capabilities, and practical applications of microscopy in various scientific fields. Learn how to achieve precise visualization, image interpretation, and research advancements. Find insightful information on advanced microscopy, imaging techniques, sample preparation, and image analysis. Topics covered include cell biology, neuroscience, and cancer research with a focus on cutting-edge applications and innovations.

Spectral separation of 11 fluorophores coupled to polystyrene beads on a STELLARIS confocal system.

Multiplexing through Spectral Separation of 11 Colors

Fluorescence microscopy is a fundamental tool for life science research that has evolved and matured together with the development of multicolor labeling strategies in cells tissues and model…

Two-color caspase assay with tile scan. U2OS cells were treated with the nuclear marker DRAQ5 (magenta) and CellEvent™ (yellow).

Following Multiple Events during Staurosporine Apoptosis

In this video on demand, we show how adding additional markers to an apoptosis kit can markedly increase the amount of information a researcher can obtain from the same experiment. The simultaneous…

Image of murine-brain tissue showing a region removed with UV laser microdissection.

RNA Quality after Different Tissue Sample Preparation

The influence of sample preparation and ultraviolet (UV) laser microdissection (UV LMD) on the quality of RNA from murine-brain tissue cryo-sections is described in this article. To obtain good…

Projection of a confocal z-stack. Sum159 cells, human breast cancer cells kindly provided by Ievgeniia Zagoriy, Mahamid Group, EMBL Heidelberg, Germany. Blue–Hoechst - indicates nuclei, Green–MitoTracker mitochondria, and red–Bodipy - lipid droplets

New Imaging Tools for Cryo-Light Microscopy

New cryo-light microscopy techniques like LIGHTNING and TauSense fluorescence lifetime-based tools reveal structures for cryo-electron microscopy.

Transverse histological cut of a rabbit tongue. 50 Mpixels images (2326 µm x 1739 µm) in 14 x 18 tiles. Lifetime gives an additional contrast that allows to differentiate different structures in histological stainings.

A Guide to Fluorescence Lifetime Imaging Microscopy (FLIM)

The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.

Untreated Hela Kyoto cells stained to show the nucleus (Hoechst, blue), the cis-golgi matrix protein GM130 (AF488, green), and the trans-golgi network membrane protein TGN46 (AF647, red).

Golgi Organizational Changes in Response to Cell Stress

In this video on demand, our special guest George Galea from EMBL Heidelberg will look at HeLa Kyoto cells treated with various chemotherapeutic agents to investigate their effect on the Golgi complex…

Pancreatic ductal adenocarcinoma tissue section imaged with Cell DIVE

Multiplexed Imaging Types, Benefits and Applications

Multiplexed imaging is an emerging and exciting way to extract information from human tissue samples by visualizing many more biomarkers than traditional microscopy. By observing many biomarkers…

3D Reconstruction of brain slide image_Mica

3D Tissue Imaging: From Fast Overview To High Resolution With One Click

3D Tissue imaging is a widespread discipline in the life sciences. Researchers use it to reveal detailed information of tissue composition and integrity, to make conclusions from experimental…

How To Perform Fast & Stable Multicolor Live-Cell Imaging

With the help of live-cell imaging researchers gain insights into dynamic processes of living cells up to whole organisms. This includes intracellular as well as intercellular activities. Protein or…