Perform multiplexed antibody-based tissue imaging quickly and cheaply with IBEX
In recent years, the expansion of multiplexed imaging platform has been remarkable. Multiplexed antibody-based imaging enables the characterization of cellular interactions, spatial relationships, and histological patterns in tissues. These techniques require considerable resources and domain expertise for implementation, which limit their widespread adoption. To overcome these challenges, we developed Iterative Bleaching Extends multi-pleXity (IBEX). IBEX is an open-source method that can be completed at relatively low cost in 2-5 days by biologists with basic laboratory skills.
In addition to cost-effective techniques such as IBEX, community initiatives are needed to extend the reach of multiplexed tissue imaging. The IBEX Imaging Community is an international group of scientists committed to sharing knowledge related to multiplexed imaging in a transparent and collaborative manner. Organ Mapping Antibody Panels (OMAPs) are community-validated resources that save time and money, increase reproducibility, as well as support the construction of a Human Reference Atlas.
This webinar will provide guidelines for the successful application of IBEX to a wide range of scientific questions using diverse imaging platforms. A significant amount of time will be devoted to the following topics: optimal tissue processing, working with fixed frozen and FFPE specimens, identifying appropriate reagents, and constructing organ atlases. Team science initiatives will be highlighted to encourage attendees to join and accelerate discovery together.
