Fixation and Dehydration
3% Glutaraldehyde in PBS, pH 7.3 at 4°C | 16 h |
Distilled water | 3x10 min. |
1% aqueous OsO4, at 4°C | 16 h |
Distilled water | 3x10 min. |
Ethanol series: 30%, 50%, 70%, 80%, 90%, 96%, 100% at 25°C | 1x10 min. |
Acetone series: 30%, 50%, 100% | 1x10 min. |
Leica EM CPD300 auto program
Coating
Mount the dried samples on stubs containing carbon adhesives.
Platinum / Palladium coating: 6 nm
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