High-pressure freezing: Revealing functional mechanisms of synaptic transmission
Webinar on-demand
Learn more about applying optogenetic stimulation in the EM ICE and how this technology has the potential to reveal structural and functional mechanisms of synaptic transmission. Get a detailed introduction of how optogenetic stimulation is applied to intact networks in acute brains slices and organotypic brain slice cultures from mice.
Key Learnings
- Preparing brain tissue (acute slices and organotypic slice cultures) with high-pressure freezing.
- Strategies to optimize the freezing of brain tissue.
- Strategies to optimize the light stimulation of brain tissue prepared with high-pressure freezing.
- Optimized freeze substitution for brain tissue specimens after light stimulation.
- Possible future applications for brain tissue specimens prepared with high-pressure freezing and observed with light-stimulation.