Prof. Stephenson presents data from studies on zebrafish gastrulation, highlighting the use of multi-view light-sheet microscopy to track the fate of cells as they undergo specification and morphogenesis. He explains how these advanced imaging techniques have enabled researchers to create detailed fate maps with single-cell resolution, revealing how cells in early development contribute to different tissue types.
Then Dr. Boni introduces the Viventis Deep microscope from Leica Microsystems. This state-of-the-art instrument features dual-view illumination and detection in an open-top configuration, allowing for easy sample mounting and long-term imaging. He illustrates how this setup facilitates the study of various biological processes, including the development of intestinal and brain organoids. The dual-view system enhances image quality by reducing light scattering and improving penetration depth, making it possible to capture high-resolution images from large multicellular systems.
Dr. Boni further discusses the practical aspects of using light-sheet microscopy for long-term live imaging. The advantages of the open-top sample holder, which allows for easy pipetting and medium exchange, enables researchers to conduct multi-sample imaging experiments. The system's advanced software provides flexibility for running complex imaging protocols, including multi-position imaging and automated alignment of the light sheet.
The session concludes with a Q&A segment, where the speakers address various technical questions from the audience, like computational modeling of cell volumes, the use of ratio-metric probes for real-time imaging, and the compatibility of light-sheet microscopy with different sample preparation methods.
By enabling detailed observation of cellular dynamics and gene-expression changes, light-sheet microscopy can help scientists unravel the complex regulatory networks that govern development.
