Life Science Research

This is the place to expand your knowledge, research capabilities, and practical applications of microscopy in various scientific fields. Learn how to achieve precise visualization, image interpretation, and research advancements. Find insightful information on advanced microscopy, imaging techniques, sample preparation, and image analysis. Topics covered include cell biology, neuroscience, and cancer research with a focus on cutting-edge applications and innovations.

Virtual Reality Showcase for STELLARIS Confocal Microscopy Platform

In this webinar, you will discover how to perform 10-color acquisition using a confocal microscope. The challenges of imaged-based approaches to identify skin immune cells. A new pipeline to assess…

Combining spectrally resolved detection and fluorescence lifetime multiplexing

Live-Cell Fluorescence Lifetime Multiplexing Using Organic Fluorophores

On-demand video: Imaging more subcellular targets by using fluorescence lifetime multiplexing combined with spectrally resolved detection.

Donor (D) and acceptor (A) molecule which participate in FRET (Förster resonance energy transfer).

What is FRET with FLIM (FLIM-FRET)?

This article explains the FLIM-FRET method which combines resonance energy transfer and fluorescence lifetime imaging to study protein-protein interactions.

Visualizing Protein-Protein Interactions by Non-Fitting and Easy FRET-FLIM Approaches

The Webinar with Dr. Sergi Padilla-Parra is about visualizing protein-protein interaction. He gives insight into non-fitting and easy FRET-FLIM approaches.

Transverse histological cut of a rabbit tongue. 50 Mpixels images (2326 µm x 1739 µm) in 14 x 18 tiles. Lifetime gives an additional contrast that allows to differentiate different structures in histological stainings.

A Guide to Fluorescence Lifetime Imaging Microscopy (FLIM)

The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.

Fluorescence Lifetime-based Imaging Gallery

Confocal microscopy relies on the effective excitation of fluorescence probes and the efficient collection of photons emitted from the fluorescence process. One aspect of fluorescence is the emission…

How to Quantify Changes in the Metabolic Status of Single Cells

Metabolic imaging based on fluorescence lifetime provides insights into the metabolic dynamics of cells, but its use has been limited as expertise in advanced microscopy techniques was needed. Now,…

Adding Dimensions to Multiplex Molecular Imaging

Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better understand the underlying cellular and molecular mechanisms of complex…

How FLIM Microscopy Helps to Detect Microplastic Pollution

The use of autofluorescence in biological samples is a widely used method to gain detailed knowledge about systems or organisms. This property is not only found in biological systems, but also…