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Workflows and Instrumentation for Cryo-electron Microscopy

An overview - Webinar on-demand

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Cryo-electron microscopy is an increasingly popular modality to study the structures of macromolecular complexes and has enabled numerous new insights in cell biology. In recent years, cryo-electron microscopy has expanded further towards in situ structural biology and has become the go-to technique for resolving structures in their native context. Similarly, freeze-fracturing and cryo-scanning electron microscopy (SEM) have grown in popularity.

Receive a full overview of cryo-preparation instruments, including vitrification, light microscopy screening, sectioning, planing, fracturing, milling, coating and transfer in our on-demand Webinar. All of these steps happen under cryogenic conditions while minimizing contamination and without risk of devitrification. We discuss several cryo-workflows to explain the full potential of the individual instruments.

Key Learnings

  • Optimal vitrification of biological samples.
  • Cryo-electron microscopy of vitreous sections (CEMOVIS) of large samples.
  • Screening vitrified specimens using cryo-fluorescence microscopy.
  • Freeze fracturing and cryo-coating.
  • Cryo transfer for high vacuum instruments.

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