Science Lab

Learn. Share. Contribute. The knowledge portal of Leica Microsystems. Find scientific research and teaching material on the subject of microscopy. The portal supports beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies. Become part of the Science Lab community and share your expertise.

Images of smooth muscle cells during wound healing. Courtesy L.S. Shankman, Ph.D., University of Virginia.

Studying Wound Healing of Smooth Muscle Cells

This article discusses how wound healing of cultured smooth muscle cells (SMCs) in multiwell plates can be reliably studied over time with less effort using a specially configured Leica inverted…

Cellular microtubule network in a fibroblast cell.

How to Prepare your Specimen for Immunofluorescence Microscopy

Immunofluorescence (IF) is a powerful method for visualizing intracellular processes, conditions and structures. IF preparations can be analyzed by various microscopy techniques (e.g. CLSM,…

Fluorescent Dyes

A basic principle in fluorescence microscopy is the highly specific visualization of cellular components with the help of a fluorescent agent. This can be a fluorescent protein – for example GFP –…

Virally labeled neurons (red) and astrocytes (green) in a cortical spheroid derived from human induced pluripotent stem cells. THUNDER Model Organism Imager with a 2x 0.15 NA objective at 3.4x zoom was used to produce this 425 µm Z-stack (26 positions), which is presented here as an Extended Depth of Field (EDoF) projection. Images courtesy of Dr. Fikri Birey from the Dr. Sergiu Pasca laboratory at Stanford University, 3165 Porter Dr., Palo Alto, CA

Download The Guide to Live Cell Imaging

In life science research, live cell imaging is an indispensable tool to visualize cells in a state as in vivo as possible. This E-book reviews a wide range of important considerations to take to…