Leica Microsystems: Leica Science Lab

Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!

Gene Editing with CRISPR/Cas9 - Breakthrough in Genome Engineering

The CRISPR/Cas9 system is one of several different bacterial systems for defense against viral attacks. It consists of two main components. One is a small piece of RNA which binds to the viral target…

Paper Samples - Sample Preparation for SEM

Application Note for Leica EM RES102 - A coated paper sample has been prepared with ion beam slope cutting in order to test the procedure with regard to its applicability. With the use of ion beam…

Transgenic zebrafish larva where fluorescent proteins label the heart muscle blue, blood and blood vessels red, and all circulatory system cells green. Image recorded with a M205 FA microscope.

Imaging and Analyzing Zebrafish, Medaka, and Xenopus

Discover how to image and analyze zebrafish, medaka, and Xenopus frog model organisms efficiently with a microscope for developmental biology applications from this article.

Bacteria Protocol - Critical Point Drying of E. coli for SEM

Application Note for Leica EM CPD300 - Critical point drying of E. coli with subsequent platinum / palladium coating and SEM analysis. Sample was inserted into a filter disc (Pore size: 16 - 40 μm)…

Successful Endodontic Treatment with Dental Operating Microscopes

In endodontics, accurate treatment is not only dependent on the technical skills and knowledge of the dentist, but also on clear, detailed visualization of the surgical field. As the outcome of an…

Fluorescence stereo microscope image of anesthetized Mediterranean fruit flies recorded with a M205 stereo microscope.

Investigating Fruit Flies (Drosophila melanogaster)

Learn how to image and investigate Drosophila fruit fly model organisms efficiently with a microscope for developmental biology applications from this article.

Studying Caenorhabditis elegans (C. elegans)

Find out how you can image and study C. elegans roundworm model organisms efficiently with a microscope for developmental biology applications from this article.

BABB Clearing and Imaging for High Resolution Confocal Microscopy

Multipohoton microscopy experiment using Leica TCS SP8 MP and Leica 20x/0.95 NA BABB immersion objective. Understanding kidney microanatomy is key to detecting and identifying early events in kidney…

Ion Beam Polishing of Sample Surfaces - Sample Preparation for SEM

Application Note for Leica EM RES102 - Ion milling can be used to reduce the roughness of sample surfaces. Small angles less than 6° with respect to the sample surface are necessary. The high voltage…