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Science Lab

Science Lab

Science Lab

The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!
Fluorescence microscope image of a life-science specimen

An Introduction to Fluorescence

This article gives an introduction to fluorescence and photoluminescence, which includes phosphorescence, explains the basic theory behind them, and how fluorescence is used for microscopy.
Masson-Goldner staining of a hedgehog brain slice.

How to Image Histological and Fluorescent Samples with One System

VIDEO ON DEMAND - How to image histological and fluorescent samples with one system. FluoSync, the new technology embedded into Mica enables the imaging of both histological staining and fluorescence…

How to Radically Simplify Workflows in Your Imaging Facility

VIDEO ON DEMAND - How to radically simplify imaging workflows and generate meaningful results with less time and effort using a highly automated microscope that unites widefield and confocal imaging.

FluoSync - a Fast & Gentle Method for Unmixing Multicolor Images

In this white paper, we focus on a fast and reliable method for obtaining high-quality multiplex images in fluorescence microscopy. FluoSync combines an existing method for hybrid unmixing with…

Harnessing Microfluidics to Maintain Cell Health During Live-Cell Imaging

VIDEO ON DEMAND - In this webinar on-demand, we will use microfluidics to explore the effect of shear stress on cell morphology, examine the effect of nutrient replenishment on cellular growth during…
Living HeLa cells stained with WGA-488 (yellow), SPY-Actin (cyan), and SiR-Tubulin (magenta). Instant Computational Clearing (ICC) was applied.

How to Perform Dynamic Multicolor Time-Lapse Imaging

Live-cell imaging sheds light on diverse cellular events. As many of these events have fast dynamics, the microscope imaging system must be fast enough to record every detail. One major advantage of…
Two-color caspase assay with tile scan. U2OS cells were treated with the nuclear marker DRAQ5 (magenta) and CellEvent™ (yellow).

Following Multiple Events during Staurosporine Apoptosis

In this video on demand, we show how adding additional markers to an apoptosis kit can markedly increase the amount of information a researcher can obtain from the same experiment. The simultaneous…
Untreated Hela Kyoto cells stained to show the nucleus (Hoechst, blue), the cis-golgi matrix protein GM130 (AF488, green), and the trans-golgi network membrane protein TGN46 (AF647, red).

Golgi Organizational Changes in Response to Cell Stress

In this video on demand, our special guest George Galea from EMBL Heidelberg will look at HeLa Kyoto cells treated with various chemotherapeutic agents to investigate their effect on the Golgi complex…
Protist Paramecium (Paramecium tetraurelia) stained to show the nucleus

AI-Enabled Spatial Analysis of Complex 3D Datasets

This video on demand offers practical advice on the extraction of publication grade insights from microscopy images. Our special guest Luciano Lucas (Leica Microsystems) will illustrate how Mica’s…
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