Science Lab
Science Lab
The knowledge portal of Leica Microsystems offers scientific research and teaching material on the subjects of microscopy. The content is designed to support beginners, experienced practitioners and scientists alike in their everyday work and experiments. Explore interactive tutorials and application notes, discover the basics of microscopy as well as high-end technologies – become part of the Science Lab community and share your expertise!
Virtual Reality Showcase for STELLARIS Confocal Microscopy Platform
In this webinar, you will discover how to perform 10-color acquisition using a confocal microscope. The challenges of imaged-based approaches to identify skin immune cells. A new pipeline to assess…
Live-Cell Fluorescence Lifetime Multiplexing Using Organic Fluorophores
On-demand video: Imaging more subcellular targets by using fluorescence lifetime multiplexing combined with spectrally resolved detection.
What is FRET with FLIM (FLIM-FRET)?
This article explains the FLIM-FRET method which combines resonance energy transfer and fluorescence lifetime imaging to study protein-protein interactions.
Visualizing Protein-Protein Interactions by Non-Fitting and Easy FRET-FLIM Approaches
The Webinar with Dr. Sergi Padilla-Parra is about visualizing protein-protein interaction. He gives insight into non-fitting and easy FRET-FLIM approaches.
A Guide to Fluorescence Lifetime Imaging Microscopy (FLIM)
The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.
Fluorescence Lifetime-based Imaging Gallery
Confocal microscopy relies on the effective excitation of fluorescence probes and the efficient collection of photons emitted from the fluorescence process. One aspect of fluorescence is the emission…
How to Quantify Changes in the Metabolic Status of Single Cells
Metabolic imaging based on fluorescence lifetime provides insights into the metabolic dynamics of cells, but its use has been limited as expertise in advanced microscopy techniques was needed.
Now,…
Adding Dimensions to Multiplex Molecular Imaging
Molecular imaging of living specimens offers a means to draw upon the growing body of high-throughput molecular data to better understand the underlying cellular and molecular mechanisms of complex…
How FLIM Microscopy Helps to Detect Microplastic Pollution
The use of autofluorescence in biological samples is a widely used method to gain detailed knowledge about systems or organisms. This property is not only found in biological systems, but also…
