James DeRose , Ph.D.
James DeRose ist Global Marketing Communication Manager bei Leica Microsystems. Sein Schwerpunkt liegt auf der Erstellung optimierter Inhalte für Anwendungen in den Bereichen Life Science, Materialwissenschaften sowie Industrie und Fertigung. Er erstellt Applikationsberichte, Fallstudien, technische Berichte, White Papers, Anwendungsseiten, Produktseiten, Social Media Posts, E-Mails, Testimonials und andere Materialien. Er arbeitet seit 2013 bei Leica Microsystems. In der Vergangenheit arbeitete er an Anwendungsentwicklungsprojekten in den Bereichen Grenzflächenchemie und -physik, Wärme- und Verfahrenstechnik, Korrosion und Metallographie, Oberflächenbeschichtungen, Materialwissenschaften, Biotechnologie und Zellbiologie. Er hat Erfahrung mit verschiedenen Arten von Mikroskopie- und Analysemethoden.
Phasenkontrast und Mikroskopie
Dieser Artikel erklärt den Phasenkontrast, eine optische Mikroskopietechnik, die feine Details von ungefärbten, transparenten Proben sichtbar macht, die mit gewöhnlicher Hellfeldbeleuchtung schwer zu…
Immersion Objectives
How an immersion objective, which has a liquid medium between it and the specimen being observed, helps increase the numerical aperture and microscope resolution is explained in this article.
How to Determine Cell Confluency with a Digital Microscope
This article shows how to measure cell confluency in an easy and consistent way with Mateo TL, increasing confidence in downstream experiments.
Mikroskopische Auflösung: Konzepte, Faktoren und Berechnungen
Dieser Artikel erklärt in einfachen Worten mikroskopische Auflösungskonzepte wie die Airy-Scheibchen, das Abbe-Limit, das Rayleigh-Kriterium und der Halbwertsbreite (FWHM). Außerdem wird der…
![[Translate to German:] Donor (D) and acceptor (A) molecule which participate in FRET (Förster resonance energy transfer).](/fileadmin/_processed_/9/9/csm_Donor_and_acceptor_molecule_which_participate_in_FRET_8ae2ab4a65.jpg "[Translate to German:] Donor (D) and acceptor (A) molecule which participate in FRET (Förster resonance energy transfer).")
Was ist FRET mit FLIM (FLIM-FRET)?
Der Beitrag erläutert die FLIM-FRET-Methode, die Resonanzenergietransfer und Fluoreszenz-Lebensdauer-Imaging zur Untersuchung von Protein-Protein Wechselwirkungen kombiniert.
3 Factors Determine the Damage Potential of Particles
This article discusses the 3 factors for determining the potential of a particle to cause damage to parts and components in the automotive and electronic industry. These factors include the…
Studying Ocular Birth Defects
This article discusses how lens formation and ocular birth defects can be studied with sharp widefield microscopy images which are acquired rapidly. The mouse ocular lens is used as a model to study…
Deep Visual Proteomics Provides Precise Spatial Proteomic Information
Despite the availability of imaging methods and mass spectroscopy for spatial proteomics, a key challenge that remains is correlating images with single-cell resolution to protein-abundance…
RNA Quality after Different Tissue Sample Preparation
The influence of sample preparation and ultraviolet (UV) laser microdissection (UV LMD) on the quality of RNA from murine-brain tissue cryo-sections is described in this article. To obtain good…
Factors to Consider for a Cleanliness Analysis Solution
Choosing the right cleanliness analysis solution is important for optimal quality control. This article discusses the important factors that should be taken into account to find the solution that best…
Leitfaden zur Fluoreszenzlebensdauer-Imaging-Mikroskopie (FLIM)
The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.
Cleanliness Analysis for Particulate Contamination
Devices, products, and their components fabricated in many industries can be quite sensitive to contamination and, as a result, have stringent requirements for technical cleanliness. Measurement…
![[Translate to German:] Particles and fibers on a filter which will be counted and analyzed for cleanliness](/fileadmin/_processed_/8/7/csm_Particles_and_fibers_on_a_filter_5ecd4bf7cc.jpg "[Translate to German:] Particles and fibers on a filter which will be counted and analyzed for cleanliness")
Effiziente Partikelzählung und -analyse
Dieser Bericht befasst sich mit der Partikelzählung und -analyse unter Verwendung der optischen Mikroskopie bei der technischen Sauberkeitsanalyse von Teilen und Komponenten. Die Partikelzählung und…
Quality Control Under the Microscope
Fast-rising demand for electric vehicles is one of the market’s main drivers, but there are other hotspots of growth, including the rise in renewable energy installations, such as photovoltaic panels,…
Hydraulics in the Automotive and Aerospace Industries
Cleanliness standards relating to lubricants, hydraulic fluids, and oils, e.g., ISO 4406 and DIN 51455 are discussed in this article. Cleanliness plays a central role in the automotive and…
Studying Wound Healing of Smooth Muscle Cells
This article discusses how wound healing of cultured smooth muscle cells (SMCs) in multiwell plates can be reliably studied over time with less effort using a specially configured Leica inverted…
![[Translate to German:] Particles which could be found during cleanliness analysis of parts and components.](/fileadmin/_processed_/8/f/csm_Particles_found_during_cleanliness_analysis_9909ee7c63.jpg "[Translate to German:] Particles which could be found during cleanliness analysis of parts and components.")
Technische Sauberkeit von Automobilkomponenten und -teilen
In diesem Artikel werden die ISO-Norm 16232 und die VDA 19-Richtlinien erläutert und die Verfahren zur Partikelanalyse kurz zusammengefasst. Diese liefern wichtige Kriterien für die Sauberkeit von…
Multi-Color Caspase 3/7 Assays with Mica
Caspases are involved in apoptosis and can be utilized to determine if cells are undergoing this programmed cell death pathway in so-called caspase assays. These assays can be run by e.g. flow…
Fast, High-contrast 3D Imaging of Sensory Neurons
This article discusses how fast, high-contrast 3D imaging of dorsal root ganglion (DRG) tissue with a THUNDER Imager Tissue using large volume computational clearing (LVCC) allows sensory neurons to…
Find Relevant Specimen Details from Overviews
Switch from searching image by image to seeing the full overview of samples quickly and identifying the important specimen details instantly with confocal microscopy. Use that knowledge to set up…
Verifying Specifications for Auto Parts and Components
During the development and production of automotive parts and components, whether by suppliers or the auto manufacturer, specifications must be met. This goal is important, because the specifications…
Top Challenges for Visual Inspection
This article discusses the challenges encountered when performing visual inspection and rework using a microscope. Using the right type of microscope and optical setup is paramount in order to…
![[Translate to German:] Routine inspection microscope Ivesta 3](/fileadmin/_processed_/a/a/csm_Ivesta_3_integrated_monitor_users_2_dc18a68be8.jpg "[Translate to German:] Routine inspection microscope Ivesta 3")
How to select the right solution for visual inspection
This article helps users with the decision-making process when selecting a microscope as a solution for routine visual inspection. Important factors that should be considered are described.
Introduction to 21 CFR Part 11 and Related Regulations
This article provides an overview of regulations and guidelines for electronic records (data entry, storage, signatures, and approvals) used in the USA (21 CFR Part 11), EU (GMP Annex 11), and China…
Why is Manual Visual Inspection of Medical Devices so Challenging?
This article discusses how manual visual inspection, which is prevalent in the medical device industry, can lead to inconsistent results. It also addresses the challenges quality managers and…
Studying Cell Division
Cell division is a biological process during which all cellular components must be distributed among the daughter cells. The division process requires firm coordination for success. Microscopy is…
Investigating Synapses in Brain Slices with Enhanced Functional Electron Microscopy
A fundamental question of neuroscience is: what is the relationship between structural and functional properties of synapses? Over the last few decades, electrophysiology has shed light on synaptic…
Topographic Analysis of Firing Pin Impressions on Cartridge Cases
The analysis of fired cartridges for primer cup morphology and flattening and firing pin impression (crater) depth using topographic data is discussed in this article. Topographical analysis of the…
Studying Pulmonary Fibrosis
The results shown in this article demonstrate that fibrotic and non-fibrotic regions of collagen present in mouse lung tissue can be distinguished better with polarized light compared to brightfield.…
Keeping Particulate Contamination Under Control in Pharmaceutical Products
This article describes how a 2-methods-in-1 solution combining optical microscopy and laser induced breakdown spectroscopy (LIBS) can be utilized for identification of particulate contaminants in the…
