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Science Lab
Bienvenido al portal de conocimiento de Leica Microsystems. Aquí encontrará investigación científica y material didáctico sobre el tema de la microscopía. El portal ayuda a principiantes, profesionales experimentados y científicos por igual en su trabajo diario y en sus experimentos. Explore tutoriales interactivos y notas de aplicación, descubra los fundamentos de la microscopía, así como las tecnologías de gama alta. Forme parte de la comunidad Science Lab y comparta sus conocimientos.
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Introduction to Mammalian Cell Culture
Mammalian cell culture is one of the basic pillars of life sciences. Without the ability to grow cells in the lab, the fast progress in disciplines like cell biology, immunology, or cancer research…
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Collecting Light: The Importance of Numerical Aperture in Microscopy
Numerical aperture (abbreviated as ‘NA’) is an important consideration when trying to distinguish detail in a specimen viewed down the microscope. NA is a number without units and is related to the…
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Laser Beam Shaping for Multicolor Multiphoton Microscopy
Multiphoton Microscopy is one of the current hot topics in life science research. The new Leica TCS SP8 DIVE from Leica Microsystems presents a series of beneficial new innovations, including a freely…
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Introduction to Widefield Microscopy
This article gives an introduction to widefield microscopy, one of the most basic and commonly used microscopy techniques. It also shows the basic differences between widefield and confocal…
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Optimization of the Interplay of Optical Components for Aberration Free Microscopy
Optical microscopes are used to magnify objects which are otherwise invisible for the human eye. For this purpose high quality optics is necessary to achieve appropriate resolution. However, besides…
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Primary Beam Splitting Devices for Confocal Microscopes
Current fluorescence microscopy employs incident illumination which requires separation of illumination and emission light. The classical device performing this separation is a color-dependent beam…
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Photoactivatable, Photoconvertible, and Photoswitchable Fluorescent Proteins
Fluorescent proteins (FPs) such as GFP, YFP or DsRed are powerful tools to visualize cellular components in living cells. Nevertheless, there are circumstances when classical FPs reach their limits.…
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Pinhole Effect in Confocal Microscopes
When operating a confocal microscope, or when discussing features and parameters of such a device, we inescapably mention the pinhole and its diameter. This short introductory document is meant to…
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Rodent and Small-Animal Surgery
Learn how you can perform rodent (mouse, rat, hamster) and small-animal surgery efficiently with a microscope for developmental biology and medical research applications by reading this article.