Scienze della vita

Scienze della vita

Scienze della vita

Questo è il posto giusto per ampliare le vostre conoscenze, le capacità di ricerca e le applicazioni pratiche della microscopia in vari campi scientifici. Imparate come ottenere una visualizzazione precisa, l'interpretazione delle immagini e i progressi della ricerca. Troverete informazioni approfondite sulla microscopia avanzata, sulle tecniche di imaging, sulla preparazione dei campioni e sull'analisi delle immagini. Gli argomenti trattati comprendono la biologia cellulare, le neuroscienze e la ricerca sul cancro, con particolare attenzione alle applicazioni e alle innovazioni più avanzate.
Complete camera overview of EM grid recorded with 3 channels. Inserts displaying the positions, where superresolved 3D confocal images were recorded. 3D renderings of these positions are shown in the zoomed inserts. Fluorescence channels (nuclei by Hoechst, blue; mitochondria by MitoTracker Green, green; lipid Droplets by Bodipy and Crimson Beads, red). Width of a grid square is 90 ?m, width of a grid bar is 35 ?m. Samples kindly provided by Ievgeniia Zagoriy, Mahamid-Group, EMBL Heidelberg, Germany.

From Bench to Beam: A Complete Correlative Cryo Light Microscopy Workflow

In the webinar entitled "A Multimodal Vitreous Crusade, a Cryo Correlative Workflow from Bench to Beam" a team of experts discusses the exciting world of correlative workflows for structural biology…

Tomografica Crioelettronica

La tomografia crioelettronica (CryoET) viene utilizzata per rilevare le biomolecole all'interno del proprio ambiente cellulare fino a una risoluzione senza precedenti, inferiore a un nanometro.
Projection of a confocal z-stack. Sum159 cells, human breast cancer cells kindly provided by Ievgeniia Zagoriy, Mahamid Group, EMBL Heidelberg, Germany. Blue–Hoechst - indicates nuclei, Green–MitoTracker mitochondria, and red–Bodipy - lipid droplets

New Imaging Tools for Cryo-Light Microscopy

New cryo-light microscopy techniques like LIGHTNING and TauSense fluorescence lifetime-based tools reveal structures for cryo-electron microscopy.
Correlation of markers in the LM and the FIB image.

How to Target Fluorescent Structures in 3D for Cryo-FIB Milling

This article describes the major steps of the cryo-electron tomography workflow including super-resolution cryo-confocal microscopy. We describe how subcellular structures can be precisely located in…
HeLa cells labeled with dark blue – Hoechst, Nuclei; magenta – MitoTracker Green, Mitochondria; turquoise - Bodipy, lipid droplets. Cells kindly provided by Ievgeniia Zagoriy, Mahamid Group, EMBL Heidelberg, Germany.

Precise 3D Targeting for EM Imaging - Access What Matters

Find out how the seamless cryo-electron tomography workflow Coral Cryo uses confocal super resolution to target your structure of interest more precisely.

The Cryo-CLEM Journey

This article describes the Cryo-CLEM technology and the benefits it can provide for scientists. Additionally, some scientific publications are highlighted. Recent developments in cryo electron…
Cryo FIB lamella - Overlay of SEM and confocal fluorescence image. Target structure in yeast cells (nuclear pore proteine Nup159-Atg8-split Venus, red) marked by an arrow. Scale bar: 5 µm. Alegretti et al., Nature 586, 796-800 (2020).

Targeting Active Recycling Nuclear Pore Complexes using Cryo Confocal Microscopy

In this article, how cryo light microscopy and, in particular cryo confocal microscopy, is used to improve the reliability of cryo EM workflows is described. The quality of the EM grids and samples is…

Advancing Cell Biology with Cryo-Correlative Microscopy

Correlative light and electron microscopy (CLEM) advances biological discoveries by merging different microscopes and imaging modalities to study systems in 4D. Combining fluorescence microscopy with…

Improve Cryo Electron Tomography Workflow

Leica Microsystems and Thermo Fisher Scientific have collaborated to create a fully integrated cryo-tomography workflow that responds to these research needs: Reveal cellular mechanisms at…
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