Scienze della vita

Scienze della vita

Scienze della vita

Questo è il posto giusto per ampliare le vostre conoscenze, le capacità di ricerca e le applicazioni pratiche della microscopia in vari campi scientifici. Imparate come ottenere una visualizzazione precisa, l'interpretazione delle immagini e i progressi della ricerca. Troverete informazioni approfondite sulla microscopia avanzata, sulle tecniche di imaging, sulla preparazione dei campioni e sull'analisi delle immagini. Gli argomenti trattati comprendono la biologia cellulare, le neuroscienze e la ricerca sul cancro, con particolare attenzione alle applicazioni e alle innovazioni più avanzate.

Virologia

L’oggetto della tua ricerca si concentra su infezioni e malattie virali? Scopri in che modo puoi approfondire l’aspetto virologico utilizzando le soluzioni di imaging e la preparazione del campione di…
In vivo imaging of a mouse pial and cortical vasculature through a glass window (ROSAmT/mG::Pdgfb-CreERT2 mouse meningeal and cortical visualization following tamoxifen induction and craniotomy). Courtesy: Thomas Mathivet, PhD

Windows on Neurovascular Pathologies

Discover how innate immunity can sustain deleterious effects following neurovascular pathologies and the technological developments enabling longitudinal studies into these events.
Lifetime-based multiplexing in live cells using TauSeparation. Mammalian cells expressing LifeAct-GFP (ibidi GmbH) and labelled with MitoTracker Green. Acquisition with one detector, intensity information shown in grey. The two markers can be separated using lifetime information: LifeAct-GFP (cyan), MitoTracker Green (magenta). Image acquired with STELLARIS 5.

The Power of Reproducibility, Collaboration and New Imaging Technologies

In this webinar you willl learn what impacts reproducibility in microscopy, what resources and initiatives there are to improve education and rigor and reproducibility in microscopy and how…
Combining spectrally resolved detection and fluorescence lifetime multiplexing

Live-Cell Fluorescence Lifetime Multiplexing Using Organic Fluorophores

On-demand video: Imaging more subcellular targets by using fluorescence lifetime multiplexing combined with spectrally resolved detection.
Donor (D) and acceptor (A) molecule which participate in FRET (Förster resonance energy transfer).

What is FRET with FLIM (FLIM-FRET)?

This article explains the FLIM-FRET method which combines resonance energy transfer and fluorescence lifetime imaging to study protein-protein interactions.

Visualizing Protein-Protein Interactions by Non-Fitting and Easy FRET-FLIM Approaches

The Webinar with Dr. Sergi Padilla-Parra is about visualizing protein-protein interaction. He gives insight into non-fitting and easy FRET-FLIM approaches.
Transverse histological cut of a rabbit tongue. 50 Mpixels images (2326 µm x 1739 µm) in 14 x 18 tiles. Lifetime gives an additional contrast that allows to differentiate different structures in histological stainings.

A Guide to Fluorescence Lifetime Imaging Microscopy (FLIM)

The fluorescence lifetime is a measure of how long a fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon.
Identification of distinct structures_roundworm_Ascaris_female

Find Relevant Specimen Details from Overviews

Switch from searching image by image to seeing the full overview of samples quickly and identifying the important specimen details instantly with confocal microscopy. Use that knowledge to set up…

How to Quantify Changes in the Metabolic Status of Single Cells

Metabolic imaging based on fluorescence lifetime provides insights into the metabolic dynamics of cells, but its use has been limited as expertise in advanced microscopy techniques was needed. Now,…
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