Leica LMD6 & LMD7 レーザーマイクロダイセクション

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Download the complete list of LMD publications in "Downloads"

Single Cell Applications

Deep Visual Proteomics maps proteotoxicity in a genetic liver disease
Rosenberger FA, Mädler SC, Thorhauge KH, Steigerwald S, Fromme M, Lebedev M, Weiss CAM, Oeller M, Wahle M, Metousis A, Zwiebel M, Schmacke NA, Detlefsen S, Boor P, Fabián O, Fraňková S, Krag A, Strnad P, Mann M
Nature. 2025 Apr 16. doi: 10.1038/s41586-025-08885-4.

Human colonic EVs induce murine enteric neuroplasticity via the lncRNA GAS5/miR-23/NMDA NR2B axis
Zhou Q, Yang L, Verne ZT, Zhang BB, Fields JZ, Thacker AT, Verne GN
JCI Insight. 2025 Mar 10;10(5):e178631. doi: 10.1172/jci.insight.178631.

Deep visual multi-omics profiling reveals mechanisms that underly cancer cell differentiation and aggressiveness in clear cell renal cell carcinoma
Bolck HA, Migh E, Kriston A, Zajac NH, Kreutzer S, Tofu T, Leary P. Kovacs F, Rutishauser D, Pfammatter S, Grossmann J, Lichtfeld C, Buljan M, Rupp NJ, Horvath P, Moch H
bioRxiv, 5. Feb 2025

DNA Research

Two-hit events occurred independently in bilateral breast cancer of a heterozygous double germline pathogenic variants carrier of BRCA1 and BRCA2
Semba R, Eguchi H, Takatsu M, Hashizume T, Moteki H, Maeno K, Murakami F, Watanabe J, Kutomi G, Arai M
Research Square, 1 Apr 2025

Neural tube defects induce abnormal astrocyte development by activation and epigenetic permissiveness of STAT3
Janik K, Jin LQ, Kyzy KZ, Kaminski R, Smith GM, Krynska B
Exp Neurol. 2025 Mar 30;389:115231. doi: 10.1016/j.expneurol.2025.115231.

Selection for somatic escape variants in SERPINA1 in the liver of patients with alpha-1 antitrypsin deficiency
Brzozowska N, Wu LYD, Khodzhaeva V, Griffiths WJ, Duckworth A, Jung H, Coorens THH, Hooks Y, Chambers JE, Campbell PJ, Marciniak SJ, Hoare M
Nat Genet. 2025 Mar 10. doi: 10.1038/s41588-025-02125-1.

RNA Research

The impact of disease and species differences on the intestinal CLCA4 gene expression
Teske K, Erickson NA, Huck A, Dzamukova M, Fulde M, Heinbokel T, Horst D, Klymiuk N, Pastille E, Mekes-Adamczyk A, Löhning M, Gruber AD, Glauben R, Mundhenk L
J Mol Med (Berl). 2025 Apr 12. doi: 10.1007/s00109-025-02538-9.

Integrin α11β1 as a Key Collagen Receptor in Human Skin Dermis: Insight into Fibroblast Function and Skin Dermal Aging
Quan T, Qin Z, He T, Fisher GJ
J Invest Dermatol. 2025 Mar 24:S0022-202X(25)00364-1. doi: 10.1016/j.jid.2025.03.006

Selection for somatic escape variants in SERPINA1 in the liver of patients with alpha-1 antitrypsin deficiency
Brzozowska N, Wu LYD, Khodzhaeva V, Griffiths WJ, Duckworth A, Jung H, Coorens THH, Hooks Y, Chambers JE, Campbell PJ, Marciniak SJ, Hoare M
Nat Genet. 2025 Mar 10. doi: 10.1038/s41588-025-02125-1.

Proteomics, Lipidomics, Metabolomics

Deep Visual Proteomics maps proteotoxicity in a genetic liver disease
Rosenberger FA, Mädler SC, Thorhauge KH, Steigerwald S, Fromme M, Lebedev M, Weiss CAM, Oeller M, Wahle M, Metousis A, Zwiebel M, Schmacke NA, Detlefsen S, Boor P, Fabián O, Fraňková S, Krag A, Strnad P, Mann M
Nature. 2025 Apr 16. doi: 10.1038/s41586-025-08885-4.

Real-time functional proteomics enhances therapeutic targeting in precision oncology molecular tumor boards
Hunt AL, Randall J, Mansukhani MM, Nyberg K, Nutcharoen A, Davis J, Corgiat B, Mueller C, Melvin S, Sharma M, Johnston L, Swain W, Abulez T, Bateman NW, Maxwell GL, Deeken J, Benyounes A, Petricoin EF, Cannon TL, Conrads TP
NPJ Precis Oncol. 2025 Apr 15;9(1):111. doi: 10.1038/s41698-025-00868-y.

Proteogenomic characterization of non-functional pancreatic neuroendocrine tumors unravels clinically relevant subgroups
Ji S, Cao L, Gao J, Du Y, Ye Z, Lou X, Liu F, Zhang Y, Xu J, Shi X, Wang H, Li P, Li Y, Chen H, Yang Z, Gao S, Zhang W, Huang D, Ni S, Wei M, Wang F, Wang Y, Ding T, Jing D, Fan G, Gong Z, Lu R, Qin Y, Chen J, Xu X, Wang P, Zhang B, Ding L, Robles AI, Rodriguez H, Chang DK, Hruban RH, Gao D, Gao D, Jin G, Zhou H, Wu J, Yu X 
Cancer Cell. 2025 Apr 1:S1535-6108(25)00117-5. doi: 10.1016/j.ccell.2025.03.016.

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Highlight 2025: 

Rosenberger FA, et al.
Nature (2025 Apr 16)
https://www.nature.com/articles/s41586-025-08885-4

Making space for spatial biology in the clinic
Nat Biotechnol (6 Jan2025).
https://doi.org/10.1038/s41587-024-02543-2

Highlights 2024: 

Method of the Year 2024: spatial proteomics. 
Nat Methods 21, 2195–2196 (2024). 
https://doi.org/10.1038/s41592-024-02565-3
Nordmann et al.
https://www.nature.com/articles/s41586-024-08061-0

Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8

Fluorescence Applications

Deep Visual Proteomics maps proteotoxicity in a genetic liver disease
Rosenberger FA, Mädler SC, Thorhauge KH, Steigerwald S, Fromme M, Lebedev M, Weiss CAM, Oeller M, Wahle M, Metousis A, Zwiebel M, Schmacke NA, Detlefsen S, Boor P, Fabián O, Fraňková S, Krag A, Strnad P, Mann M
Nature. 2025 Apr 16. doi: 10.1038/s41586-025-08885-4.

Deep spatial proteomics of ovarian cancer precursor lesions delineates early disease changes and cell-of-origin signatures
Makhmut A, Dragomir MP, Fritzsche S, Moebs M, Schmitt WD, Taube ET, and Coscia F
bioRxiv, 19 Mar 2025

Human colonic EVs induce murine enteric neuroplasticity via the lncRNA GAS5/miR-23/NMDA NR2B axis
Zhou Q, Yang L, Verne ZT, Zhang BB, Fields JZ, Thacker AT, Verne GN
JCI Insight. 2025 Mar 10;10(5):e178631. doi: 10.1172/jci.insight.178631.

Cancer Research

Real-time functional proteomics enhances therapeutic targeting in precision oncology molecular tumor boards
Hunt AL, Randall J, Mansukhani MM, Nyberg K, Nutcharoen A, Davis J, Corgiat B, Mueller C, Melvin S, Sharma M, Johnston L, Swain W, Abulez T, Bateman NW, Maxwell GL, Deeken J, Benyounes A, Petricoin EF, Cannon TL, Conrads TP
NPJ Precis Oncol. 2025 Apr 15;9(1):111. doi: 10.1038/s41698-025-00868-y.

Proteogenomic characterization of non-functional pancreatic neuroendocrine tumors unravels clinically relevant subgroups
Ji S, Cao L, Gao J, Du Y, Ye Z, Lou X, Liu F, Zhang Y, Xu J, Shi X, Wang H, Li P, Li Y, Chen H, Yang Z, Gao S, Zhang W, Huang D, Ni S, Wei M, Wang F, Wang Y, Ding T, Jing D, Fan G, Gong Z, Lu R, Qin Y, Chen J, Xu X, Wang P, Zhang B, Ding L, Robles AI, Rodriguez H, Chang DK, Hruban RH, Gao D, Gao D, Jin G, Zhou H, Wu J, Yu X 
Cancer Cell. 2025 Apr 1:S1535-6108(25)00117-5. doi: 10.1016/j.ccell.2025.03.016.

Two-hit events occurred independently in bilateral breast cancer of a heterozygous double germline pathogenic variants carrier of BRCA1 and BRCA2
Semba R, Eguchi H, Takatsu M, Hashizume T, Moteki H, Maeno K, Murakami F, Watanabe J, Kutomi G, Arai M
Research Square, 1 Apr 2025

Neurosciences

Neural tube defects induce abnormal astrocyte development by activation and epigenetic permissiveness of STAT3
Janik K, Jin LQ, Kyzy KZ, Kaminski R, Smith GM, Krynska B
Exp Neurol. 2025 Mar 30;389:115231. doi: 10.1016/j.expneurol.2025.115231.

In-depth and high-throughput spatial proteomics for whole-tissue slice profiling by deep learning-facilitated sparse sampling strategy
Qin R, Ma J, He F, Qin W
Cell Discov 11, 21 (2025). 

Purinergic System Transcript Changes in the Dorsolateral Prefrontal Cortex in Suicide and Major Depressive Disorder
Sahay S, Lundh AE, Sirole RP, McCullumsmith RE, and O’Donovan SM
Int. J. Mol. Sci. 2025, 26(5), 1826

Clinical/Pathology/Immunology Research Applications

Deep Visual Proteomics maps proteotoxicity in a genetic liver disease
Rosenberger FA, Mädler SC, Thorhauge KH, Steigerwald S, Fromme M, Lebedev M, Weiss CAM, Oeller M, Wahle M, Metousis A, Zwiebel M, Schmacke NA, Detlefsen S, Boor P, Fabián O, Fraňková S, Krag A, Strnad P, Mann 
Nature. 2025 Apr 16. doi: 10.1038/s41586-025-08885-4.

Toward molecular phenotyping of temporal lobe epilepsy by spatial omics
Vermeulen I, Mohren R, Neusinger M, Dancker TA, Vandenbosch M, Beckervordersandforth J, Balluff B, Van der Hel RP, Schijns OEMG, Hoogland G, Rijkers K, Cillero-Pastor B; ACE epilepsy surgery group
Epilepsia. 2025 Mar 20. doi: 10.1111/epi.18366

Fiber Type-Specific Proteomic Alterations in R349P Desminopathy Mice
Eggers B, Schork K, Turewicz M, Barkovits K, Eisenacher M, Schröder R, Clemen CS, Marcus K
Muscle Nerve. 2025 Mar 3. doi: 10.1002/mus.28379.

Developmental Research, Epigenetics & Aging

Integrin α11β1 as a Key Collagen Receptor in Human Skin Dermis: Insight into Fibroblast Function and Skin Dermal Aging
Quan T, Qin Z, He T, Fisher GJ
J Invest Dermatol. 2025 Mar 24:S0022-202X(25)00364-1. doi: 10.1016/j.jid.2025.03.006

Cancer-independent somatic mutation of the wild-type NF1 allele in normal tissues in neurofibromatosis type 1
Oliver TRW, Lawson ARJ, Lee-Six H, Tollit A, Jung H, Hooks Y, Sanghvi R, Young MD, Butler TM, Nicola PA, Treger TD, Lensing SV, Burke GAA, Aquilina K, Löbel U, Cortes-Ciriano I, Hargrave D, Jorgensen M, Jessop FA, Coorens THH, Flanagan AM, Allinson K, Martincorena I, Jacques TS, Behjati S
Nat Genet. 2025 Feb 25. doi: 10.1038/s41588-025-02097-2.

Identification of Meibomian gland stem cell populations and mechanisms of aging
Zhu X, Xu M, Portal C, Lin Y, Ferdinand A, Peng T, Morrisey EE, Dlugosz AA, Castellano JM, Lee V, Seykora JT, Wong SY, Iomini C, Millar SE
Nat Commun. 2025 Feb 15;16(1):1663. doi: 10.1038/s41467-025-56907-6.

Plant Research

Role of an endodermis-specificmiR858b-MYB1Lmodule inthe regulation of Taxol biosynthesis inTaxus mairei
Yu C, Zhang D, Zhang L, Fang Z, Zhang Y, Lin W, Ma R, Zheng M, Bai E, Shen
The plant journal, Volume122, Issue1, 05 April 2025, e70135

Microbial-type terpene synthases significantly contribute to the terpene profile of glandular trichomes of the fern Dryopteris fragrans (L.)
Chen L, Gao R, Wei G, Luo S, Köllner TG, Xu H, Jiang Y, Zhang C, Chen X, Dickschat JS, Gershenzon J, Li S, Chang Y, Chen F
Plant J. 2025 Mar;121(6):e70079. doi: 10.1111/tpj.70079.

Polyacetylenes and sesquiterpenes in Chinese traditional herb Atractylodes lancea: biomarkers and synergistic effects in red secretory cavities
Jiang D, Lin H, Liu Z, Qi K, Zhang W, Wang H, Zhang C, Zhu L, Zhu J, Zhang Y, Huang L, Wang S, Pan Y, Guo L
Mol Hortic. 2025 Feb 4;5(1):11. doi: 10.1186/s43897-024-00130-2.

Forensics

Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Miscellaneous

Topographic imaging with automatic z-axis correction of Brassica oleracea var. viridis leaves by IR-MALDESI mass spectrometry imaging
Mills Q, Ashbacher SM, Sohn AL, Muddiman DC
Anal Bioanal Chem. 2025 Mar 15. doi: 10.1007/s00216-025-05820-4.

Three-dimensional mass spectrometry imaging (3D MSI): incorporating top-hat IR-MALDESI and automatic z-axis correction
Sohn AL, Witherspoon JG, Smart RC, Muddiman DC
Anal Bioanal Chem. 2025 Mar;417(8):1649-1661. doi: 10.1007/s00216-025-05755-w.

Single-cell imaging reveals efficient nutrient uptake and growth of microalgae darkening the Greenland Ice Sheet
Halbach L, Kitzinger K, Hansen M, Littmann S, Benning L, Bradley J, Whitehouse M, Olofsson M, Mourot R, Tranter M, Kuypers M, Ellegaard-Jensen L, Anesio A
Nat Commun 16, 1521 (2025). 

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Highlight:
Multiple: Laser Microdissection Protocols for Tissue and Cell Isolation
Bio-protocol Selections | 2024
https://en.bio-protocol.org/ebookinfo.aspx?id=23

Live Cell Culture

A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX

A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023

SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416