Leica LMD6 & LMD7 レーザーマイクロダイセクション

This list presents current publications in the major application fields for laser microdissection and is updated monthly.

Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.

The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.

Download the complete list of LMD publications in "Downloads"

Single Cell Applications

Human colonic EVs induce murine enteric neuroplasticity via the lncRNA GAS5/miR-23/NMDA NR2B axis
Zhou Q, Yang L, Verne ZT, Zhang BB, Fields JZ, Thacker AT, Verne GN
JCI Insight. 2025 Mar 10;10(5):e178631. doi: 10.1172/jci.insight.178631.

Deep visual multi-omics profiling reveals mechanisms that underly cancer cell differentiation and aggressiveness in clear cell renal cell carcinoma
Bolck HA, Migh E, Kriston A, Zajac NH, Kreutzer S, Tofu T, Leary P. Kovacs F, Rutishauser D, Pfammatter S, Grossmann J, Lichtfeld C, Buljan M, Rupp NJ, Horvath P, Moch H
bioRxiv, 5. Feb 2025

Deciphering functional tumor-immune crosstalk through highly multiplexed imaging and deep visual proteomics
Zheng X, Mund A, Mann M
Molecular Cell, 14 Jan 2025, ISSN 1097-2765, DOI: 10.1016/j.molcel.2024.12.023 

DNA Research

Selection for somatic escape variants in SERPINA1 in the liver of patients with alpha-1 antitrypsin deficiency
Brzozowska N, Wu LYD, Khodzhaeva V, Griffiths WJ, Duckworth A, Jung H, Coorens THH, Hooks Y, Chambers JE, Campbell PJ, Marciniak SJ, Hoare M
Nat Genet. 2025 Mar 10. doi: 10.1038/s41588-025-02125-1.

Genomic and transcriptomic analyses of melanoma in Japanese patients reveal candidate biomarkers for immune checkpoint inhibitor responders
Fukushima S
Research Square, 18. Feb 2025

Transcription of hepatitis B surface antigen shifts from cccDNA to integrated HBV DNA during treatment
Taddese M, Grudda T, Belluccini G, Anderson M, Cloherty G, Hwang HS, Mani M, Lo CM, Esrig N, Sulkowski MS, Sterling RK, Zhang Y, Ribeiro RM, Thomas DL, Thio CL, Balagopal A
J Clin Invest. 2025 Jan 30:e184243. doi: 10.1172/JCI184243.

RNA Research

Selection for somatic escape variants in SERPINA1 in the liver of patients with alpha-1 antitrypsin deficiency
Brzozowska N, Wu LYD, Khodzhaeva V, Griffiths WJ, Duckworth A, Jung H, Coorens THH, Hooks Y, Chambers JE, Campbell PJ, Marciniak SJ, Hoare M
Nat Genet. 2025 Mar 10. doi: 10.1038/s41588-025-02125-1.

The sperm quality in DIO male mice is linked to the NF-κB signaling and Ppp2ca expression in the hypothalamus
Feng X, Xu M, Liu Y, Wang X, Duan Y, Zheng X, Yin W, Cai Y, Zhang W, Jiang Q, Pang J, Li J
ISCIENCE (2025), doi: doi.org/10.1016/ j.isci.2025.112110.

Neutralizing IL-15 Inhibits Tissue-Damaging Immune Response in Ex Vivo Cultured Untreated Celiac Intestinal Mucosa
Rotondi Aufiero V, Iacomino G, De Chiara G, Picariello E, Iaquinto G, Troncone R, Mazzarella G:
Cells. 2025; 14(3):234. 

Proteomics, Lipidomics, Metabolomics

Spatial immune scoring system predicts hepatocellular carcinoma recurrence
Jia G, He P, Dai T, Goh D, Wang J, Sun M, Wee F, Li F, Lim JCT, Hao S, Liu Y, Lim TKH, Ngo NT, Tao Q, Wang W, Umar A, Nashan B, Zhang Y, Ding C, Yeong J, Liu L, Sun C
Nature. 2025 Mar 12. doi: 10.1038/s41586-025-08668-x.

Microbial-type terpene synthases significantly contribute to the terpene profile of glandular trichomes of the fern Dryopteris fragrans (L.)
Chen L, Gao R, Wei G, Luo S, Köllner TG, Xu H, Jiang Y, Zhang C, Chen X, Dickschat JS, Gershenzon J, Li S, Chang Y, Chen F
Plant J. 2025 Mar;121(6):e70079. doi: 10.1111/tpj.70079.

In-depth and high-throughput spatial proteomics for whole-tissue slice profiling by deep learning-facilitated sparse sampling strategy
Qin R, Ma J, He F, Qin W
Cell Discov 11, 21 (2025). 

High-Efficiency Cell-Type Proteomics Strategy Deciphers Cholangiocarcinoma Fibrosis-Associated Pathological Heterogeneity
Su Z, Zhang H, Hu H, Yang Y, Huang C, Liu C, He F, Chen W
Anal Chem. 2025 Mar 3. doi: 10.1021/acs.analchem.4c06106.

When imaging and mass-spec collide - Deep Visual Proteomics (DVP) brings us the best of both worlds
Mund A
Interview, Proteomics Playbook 2025, Front Line Genomics, p. 68 ff

---

Highlight 2025: 

Making space for spatial biology in the clinic
Nat Biotechnol (6 Jan2025).
https://doi.org/10.1038/s41587-024-02543-2

Highlights 2024: 

Method of the Year 2024: spatial proteomics. 
Nat Methods 21, 2195–2196 (2024). 
https://doi.org/10.1038/s41592-024-02565-3
Nordmann et al.
https://www.nature.com/articles/s41586-024-08061-0

Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409

Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8

Fluorescence Applications

Human colonic EVs induce murine enteric neuroplasticity via the lncRNA GAS5/miR-23/NMDA NR2B axis
Zhou Q, Yang L, Verne ZT, Zhang BB, Fields JZ, Thacker AT, Verne GN
JCI Insight. 2025 Mar 10;10(5):e178631. doi: 10.1172/jci.insight.178631.

Molecularly-guided spatial proteomics captures single-cell identity and heterogeneity of the nervous system
Dutta S, Pang M, Coughlin GM, Gudavalli S, Roukes ML, Chou T-F, Gradinaru V
bioRxiv, 11 Feb 2025

Deciphering functional tumor-immune crosstalk through highly multiplexed imaging and deep visual proteomics
Zheng X, Mund A, Mann 
Molecular Cell, 14 Jan 2025, ISSN 1097-2765, DOI: 10.1016/j.molcel.2024.12.023

Cancer Research

Spatial immune scoring system predicts hepatocellular carcinoma recurrence
Jia G, He P, Dai T, Goh D, Wang J, Sun M, Wee F, Li F, Lim JCT, Hao S, Liu Y, Lim TKH, Ngo NT, Tao Q, Wang W, Umar A, Nashan B, Zhang Y, Ding C, Yeong J, Liu L, Sun C
Nature. 2025 Mar 12. doi: 10.1038/s41586-025-08668-x.

High-Efficiency Cell-Type Proteomics Strategy Deciphers Cholangiocarcinoma Fibrosis-Associated Pathological Heterogeneity
Su Z, Zhang H, Hu H, Yang Y, Huang C, Liu C, He F, Chen W
Anal Chem. 2025 Mar 3. doi: 10.1021/acs.analchem.4c06106.

Tertiary Lymphoid Structures in Pancreatic Cancer are Structurally Homologous, Share Gene Expression Patterns and B-cell Clones with Secondary Lymphoid Organs, but Show Increased T-cell Activation
Lehmann J, Thelen M, Kreer C, Schran S, Garcia-Marquez MA, Cisic I, Siepmann K, Hagen EM, Eckel HNC, Lohneis P, Kruger S, Boeck S, Ormanns S, Rudelius M, Werner J, Popp F, Klein F, von Bergwelt-Baildon MS, Bruns CJ, Quaas A, Wennhold K, Schlößer HA
Cancer Immunol Res. 2025 Mar 4;13(3):323-336. doi: 10.1158/2326-6066.CIR-24-0299.

Neurosciences

In-depth and high-throughput spatial proteomics for whole-tissue slice profiling by deep learning-facilitated sparse sampling strategy
Qin R, Ma J, He F, Qin W
Cell Discov 11, 21 (2025). 

Purinergic System Transcript Changes in the Dorsolateral Prefrontal Cortex in Suicide and Major Depressive Disorder
Sahay S, Lundh AE, Sirole RP, McCullumsmith RE, and O’Donovan SM
Int. J. Mol. Sci. 2025, 26(5), 1826

Molecularly-guided spatial proteomics captures single-cell identity and heterogeneity of the nervous system
Dutta S, Pang M, Coughlin GM, Gudavalli S, Roukes ML, Chou T-F, Gradinaru V
bioRxiv, 11 Feb 2025

Clinical/Pathology/Immunology Research Applications

Fiber Type-Specific Proteomic Alterations in R349P Desminopathy Mice
Eggers B, Schork K, Turewicz M, Barkovits K, Eisenacher M, Schröder R, Clemen CS, Marcus K
Muscle Nerve. 2025 Mar 3. doi: 10.1002/mus.28379.

Proteome of Renal Tubuli and Serum Differentiate Pre-Existing Type 2 Diabetes and Post-Transplant Diabetes in Kidney Transplant Recipients
Skandalou E, Rivedal M, Marti HP, Halden TAS, Jenssen T, Vikse BE, Åsberg A, Furriol J
Proteomics Clin Appl. 2025 Feb 24:e70000. doi: 10.1002/prca.70000.

Purinergic System Transcript Changes in the Dorsolateral Prefrontal Cortex in Suicide and Major Depressive Disorder
Sahay S, Lundh AE, Sirole RP, McCullumsmith RE, and O’Donovan SM
Int. J. Mol. Sci. 2025, 26(5), 1826

Developmental Research, Epigenetics & Aging

Cancer-independent somatic mutation of the wild-type NF1 allele in normal tissues in neurofibromatosis type 1
Oliver TRW, Lawson ARJ, Lee-Six H, Tollit A, Jung H, Hooks Y, Sanghvi R, Young MD, Butler TM, Nicola PA, Treger TD, Lensing SV, Burke GAA, Aquilina K, Löbel U, Cortes-Ciriano I, Hargrave D, Jorgensen M, Jessop FA, Coorens THH, Flanagan AM, Allinson K, Martincorena I, Jacques TS, Behjati S
Nat Genet. 2025 Feb 25. doi: 10.1038/s41588-025-02097-2.

Identification of Meibomian gland stem cell populations and mechanisms of aging
Zhu X, Xu M, Portal C, Lin Y, Ferdinand A, Peng T, Morrisey EE, Dlugosz AA, Castellano JM, Lee V, Seykora JT, Wong SY, Iomini C, Millar SE
Nat Commun. 2025 Feb 15;16(1):1663. doi: 10.1038/s41467-025-56907-6.

Multi-omics-based mapping of decidualization resistance in patients with a history of severe preeclampsia
Muñoz-Blat I, Pérez-Moraga R, Castillo-Marco N, Cordero T, Ochando A, Ortega-Sanchís S, Parras M, Monfort-Ortiz R, Satorres-Perez E, Novillo B, Perales A, Gormley M, Granados-Aparici S, Noguera R, Roson B, Fisher SJ, Simón C, Garrido-Gómez T
Nat Med. 2025 Jan 7. doi: 10.1038/s41591-024-03407-7.

Plant Research

Microbial-type terpene synthases significantly contribute to the terpene profile of glandular trichomes of the fern Dryopteris fragrans (L.)
Chen L, Gao R, Wei G, Luo S, Köllner TG, Xu H, Jiang Y, Zhang C, Chen X, Dickschat JS, Gershenzon J, Li S, Chang Y, Chen F
Plant J. 2025 Mar;121(6):e70079. doi: 10.1111/tpj.70079.

Polyacetylenes and sesquiterpenes in Chinese traditional herb Atractylodes lancea: biomarkers and synergistic effects in red secretory cavities
Jiang D, Lin H, Liu Z, Qi K, Zhang W, Wang H, Zhang C, Zhu L, Zhu J, Zhang Y, Huang L, Wang S, Pan Y, Guo L
Mol Hortic. 2025 Feb 4;5(1):11. doi: 10.1186/s43897-024-00130-2.

RNA Extraction from Rice Immature Embryo Using Laser Capture Microdissection
Tezuka T, Nosaka-Takahashi M, Yoshikawa T, Sato Y
Methods in Molecular Biology, vol 2869. (2025) in: Kawakatsu, T. (eds) Rice.

Forensics

Advancements in differentiation between sperm cells and epithelial cells for efficient forensic DNA analysis in sexual assault cases
Dash HR
Int J Legal Med. 2024 Jul 12. doi: 10.1007/s00414-024-03285-1.

Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807

Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136

General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/

Miscellaneous

Topographic imaging with automatic z-axis correction of Brassica oleracea var. viridis leaves by IR-MALDESI mass spectrometry imaging
Mills Q, Ashbacher SM, Sohn AL, Muddiman DC
Anal Bioanal Chem. 2025 Mar 15. doi: 10.1007/s00216-025-05820-4.

Three-dimensional mass spectrometry imaging (3D MSI): incorporating top-hat IR-MALDESI and automatic z-axis correction
Sohn AL, Witherspoon JG, Smart RC, Muddiman DC
Anal Bioanal Chem. 2025 Mar;417(8):1649-1661. doi: 10.1007/s00216-025-05755-w.

Dissecting apicoplast functions through continuous cultivation of Toxoplasma gondii devoid of the organelle
Chen M, Koszti SG, Bonavoglia A, Maco B, von Rohr O, Peng HJ, Soldati-Favre D, Kloehn J
Nat Commun. 2025 Mar 1;16(1):2095. doi: 10.1038/s41467-025-57302-x.

Single-cell imaging reveals efficient nutrient uptake and growth of microalgae darkening the Greenland Ice Sheet
Halbach L, Kitzinger K, Hansen M, Littmann S, Benning L, Bradley J, Whitehouse M, Olofsson M, Mourot R, Tranter M, Kuypers M, Ellegaard-Jensen L, Anesio A
Nat Commun 16, 1521 (2025). 

---

Highlight:
Multiple: Laser Microdissection Protocols for Tissue and Cell Isolation
Bio-protocol Selections | 2024
https://en.bio-protocol.org/ebookinfo.aspx?id=23

Live Cell Culture

A 3‑Dimensional Scaffolding System Recapitulates the Hierarchical Osteon Structure
Li X, Sun Y, Wang S, Si C, Li H, and Chang B
ACS Omega 2024, XXXX, XXX, XXX-XXX

A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023

SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416