Functional in toto imaging
This list presents recent publications in the main application areas of light sheet imaging with the Viventis LS1 and LS2 and is updated regularly.
Light sheet microscopy, also known as Selective Plane Illumination Microscopy (SPIM), has emerged as a powerful imaging technique in scientific research, especially for the study of living samples. This non-invasive method combines optical sectioning and low phototoxicity, allowing researchers to visualize biological processes in three dimensions with high spatiotemporal resolution. Over the years, light sheet microscopy has evolved from its early development in the late 1990s to become a cornerstone technique in several fields of study. Light sheet microscopy has transformed our understanding of developmental biology, neurobiology, and cancer research, among others, with unprecedented insights into the dynamic interactions of cells and tissues.
Publications by application:
Organoids and spheroids
Topological morphogenesis of neuroepithelial organoids
Ishihara et al.,
Nature Physics 2022
Multiscale light-sheet organoid imaging framework
de Medeiros et al.,
Nature Communication 2022
Lineage recording in human cerebral organoids
He et al.,
Nature Methods 2021
Oocytes and Embryos
Mechanism of spindle pole organization and instability in human oocytes
So et al.,
Science 2022
Aurora kinase A is essential for meiosis in mouse oocytes
Blengini et al.,
Plos Genetics 2021
Fracking and Ostwald ripening position the lumen of the mouse blastocyst
Dumortier et al.,
Science 2019
Zebrafish and other model organisms
Left-right symmetry of zebrafish embryos requires somite surface tension
Naganathan et al.,
Nature 2022
Deconstructing body axis morphogenesis in zebrafish embryos using robot-assisted tissue micromanipulation
Ozelci et al.,
Nature Communications 2022
By year of publication:
2024 [LS2]
Open-top multi sample dual-view light-sheet microscope for live imaging of large multicellular systems
Moos et al.,
Nature Methods 2024
2024 [LS1]
LiverZap: a chemoptogenetic tool for global and locally restricted hepatocyte ablation to study cellular behaviours in liver regeneration
Ambrosio et al.,
Development 2024
Cell-autonomous generation of the wave pattern within the vertebrate segmentation clock
Rohde et al.,
Elife 2024
2023 [LS1]
Integrating single-cell imaging and RNA sequencing datasets links differentiation and morphogenetic dynamics of human pancreatic endocrine progenitors
Beydag-Tasöz et al.,
Developmental Cell 2023
CHK1-CDC25A-CDK1 regulate cell cycle progression and protect genome integrity in early mouse embryos
Knoblochova et al.,
Embo Reports 2023
Actin-driven chromosome clustering facilitates fast and complete chromosome capture in mammalian oocytes
Harasimov et al.,
Nature Cell Biology 2023
The nuclear to cytoplasmic ratio drives cellularization in the close animal relative Sphaeroforma arctica
Olivetta et al.,
Current Biology 2023
Cell fragmentation in mouse preimplantation embryos induced by ectopic activation of the polar body extrusion pathway
Pelzer et al.,
Embo Journal 2023
Inverse blebs operate as hydraulic pumps during mouse blastocyst formation
Schliffka et al.,
bioRvix 2023
Morphodynamics of human early brain organoid development
Jain et al.,
bioRvix 2023
2022 [LS1]
Deconstructing body axis morphogenesis in zebrafish embryos using robot-assisted tissue micromanipulation
Ozelci et al.,
Nature Communications 2022
Topological morphogenesis of neuroepithelial organoids
Ishihara et al.,
Nature Physics 2022
Multiscale light-sheet organoid imaging framework
de Medeiros et al.,
Nature Communication 2022
Left-right symmetry of zebrafish embryos requires somite surface tension
Naganathan et al.,
Nature 2022
Mechanism of spindle pole organization and instability in human oocytes
So et al.,
Science 2022
2021 [LS1]
Cell fate coordinates mechano-osmotic forces in intestinal crypt formation
Yang et al.,
Nature cell Biology 2021
Lineage recording in human cerebral organoids
He et al.,
Nature Methods 2021
Tissue Engineering with Mechanically Induced Solid-Fluid Transitions
Mailand et al.,
Ad. Materials 2021
Aurora kinase A is essential for meiosis in mouse oocytes
Blengini et al.,
Plos Genetics 2021
2020 [LS1]
Embryonic organoids recapitulate early heart organogenesis
Rossi et al.,
Cell Stem Cell 2020
2019 [LS1]
Self-organization and symmetry breaking in intestinal organoids development
Serra et al.,
Nature 2019
Fracking and Ostwald ripening position the lumen of the mouse blastocyst
Dumortier et al.,
Science 2019
Primed Track, high-fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins
Welling et al.,
Elife 2019.
Mitochondria in Embryogenesis: An Organellogenesis Perspective
Arribat et al.,
Frontiers in Cell and Developmental Biology 2019.
