ライフサイエンス

ライフサイエンス

ライフサイエンス

様々な科学分野における顕微鏡の知識、研究技術、そして実用的な応用を深めるための場です。正確な観察、画像解析、そして研究の進歩を実現する方法を学びましょう。高度な顕微鏡技術、イメージング技術、サンプル前処理、画像解析に関する専門的な知見を提供します。最先端のアプリケーションやイノベーションを中心に、細胞生物学、神経科学、がん研究などの分野を幅広くカバーしています。
Mouse liver (immersion fixed)

Epoxy Resin Embedding of Animal and Human Tissues for Pathological Diagnosis and Research

Application Note for Leica EM AMW - The tissues were fixed in the modified Karnovsky fixative generally by immersion overnight (at minimum 4h at room temperature). Then pieces of approx. 1mm3 were cut…

Brief Introduction to Freeze Substitution

Freeze-substitution is a process of dehydration, performed at temperatures low enough to avoid the formation of ice crystals and to circumvent the damaging effects observed after ambient-temperature…
Structural details of the C. elegans, head in cross-section. Courtesy of Müller-Reichert T, MPI-CBG, Dresden, Germany, and McDonald K, University of California, Berkeley, USA.

Brief Introduction to High-Pressure Freezing

Water is the most abundant cellular constituent and therefore important for preserving cellular ultra-structure. Currently the only way to fix cellular constituents without introducing significant…

Perusing Alternatives for Automated Staining of TEM Thin Sections

Contrast in transmission electron microscopy (TEM) is mainly produced by electron scattering at the specimen: Structures that strongly scatter electrons are referred to as electron dense and appear as…

An Introduction to CARS Microscopy

CARS overcomes the drawbacks of conventional staining methods by the intrinsic characteristics of the method. CARS does not require labeling because it is highly specific to molecular compounds which…
Scheme of a 2D mosaic scan. Drosophila melanogaster (eye section)

Mosaic Images

Confocal laser scanning microscopes are widely used to create highly resolved 3D images of cells, subcellular structures and even single molecules. Still, an increasing number of scientists are…
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