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TauSTED 775 resolves the intricate cytoskeleton network labeled with SiR-tubulin (glow - Spyrochrome), and trafficking vesicles labeled with CF594 (cyan - Biotium).

A Guide to Super-Resolution

Find out more about Leica super-resolution microscopy solutions and how they can empower you to visualize in fine detail subcellular structures and dynamics.

Universal PAINT – Dynamic Super-Resolution Microscopy

Super-resolution microscopy techniques have revolutionized biology for the last ten years. With their help cellular components can now be visualized at the size of a protein. Nevertheless, imaging…

Sample Preparation for GSDIM Localization Microscopy – Protocols and Tips

The widefield super-resolution technique GSDIM (Ground State Depletion followed by individual molecule return) is a localization microscopy technique that is capable of resolving details as small as…

Controlling the TIRF Penetration Depth is Mandatory for Reproducible Results

The main feature of total internal reflection fluorescence (TIRF) microscopy is the employment of an evanescent wave for the excitation of fluorophores instead of using direct light. A property of the…

TIRF image of brest carcinoma tumor cells expressing GFP tagged cell adhesion Molecule CD44 that is expressed on the cell membrane, imagined in TIRF.

Total Internal Reflection Fluorescence (TIRF) Microscopy

Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy…

Super-Resolution GSDIM Microscopy

The nanoscopic technique GSDIM (ground state depletion microscopy followed by individual molecule return) provides a detailed image of the spatial arrangement of proteins and other biomolecules within…

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