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The Fundamentals and History of Fluorescence and Quantum Dots

At some point in your research and science career, you will no doubt come across fluorescence microscopy. This ubiquitous technique has transformed the way in which microscopists can image, tag and…

Practical Applications of Broad Ion Beam Milling

Mechanical polishing can be time consuming and frustrating. It can also introduce unwanted artifacts when preparing cross-sectioned samples for electron backscatter diffraction (EBSD) in the scanning…
Multiphoton microscopy of an unstained mouse skin section acquired using the 4Tune detector.

Mission Impossible Accomplished: Tunable Colors for Non-descanning Detection

Leica Microsystems’ 4Tune detector, the key component of the SP8 DIVE Deep In Vivo Explorer, provides spectrally tunable image recording with non-descanning detection. An innovative solution for…
„Confetti-Mouse“, taken with the 4Tune spectral detector of Leica’s Deep in-vivo Explorer SP8 DIVE.

Laser Beam Shaping for Multicolor Multiphoton Microscopy

Multiphoton Microscopy is one of the current hot topics in life science research. The new Leica TCS SP8 DIVE from Leica Microsystems presents a series of beneficial new innovations, including a freely…

Primary Beam Splitting Devices for Confocal Microscopes

Current fluorescence microscopy employs incident illumination which requires separation of illumination and emission light. The classical device performing this separation is a color-dependent beam…
Dronpa chromophore

Photoactivatable, Photoconvertible, and Photoswitchable Fluorescent Proteins

Fluorescent proteins (FPs) such as GFP, YFP or DsRed are powerful tools to visualize cellular components in living cells. Nevertheless, there are circumstances when classical FPs reach their limits.…
Pinhole diameter and diffraction pattern.

Pinhole Effect in Confocal Microscopes

When operating a confocal microscope, or when discussing features and parameters of such a device, we inescapably mention the pinhole and its diameter. This short introductory document is meant to…
Comparison when observing a rodent model organism with a Greenough versus CMO (common main objective) stereo microscope for a task like surgery.

Rodent and Small-Animal Surgery

Learn how you can perform rodent (mouse, rat, hamster) and small-animal surgery efficiently with a microscope for developmental biology and medical research applications by reading this article.
Left: Tissue cells marked with an immunolabel (FITC) illuminated with wide-band UV excitation. Note the tissue structure with blue autofluorescence. Right: Same tissue and same immunostaining with FITC label illuminated with epi-illumination using narrow-band blue (490 nm) light. Note the increased image contrast (Ploem, 1967)

Milestones in Incident Light Fluorescence Microscopy

Since the middle of the last century, fluorescence microscopy developed into a bio scientific tool with one of the biggest impacts on our understanding of life. Watching cells and proteins with the…
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