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Bridging Structure and Dynamics at the Nanoscale through Optogenetics and Electrical Stimulation
Nanoscale ultrastructural information is typically obtained by means of static imaging of a fixed and processed specimen. However, this is only a snapshot of one moment within a dynamic system in…
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How To Create EDOF (Extended Depth of Focus) Images
Watch this video to see how you can rapidly record sharp optical microscope images of samples with a large height variation. This is done with the optional Extended Depth of Focus (EDOF) function of…
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Zebrafish Brain - Whole Organ Imaging at High Resolution
Structural information is key when one seeks to understand complex biological systems, and one of the most complex biological structures is the vertebrate central nervous system. To image a complete…
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How to make a fast Z-stack
Save time for your 2D and 3D analysis. Watch this video to learn about the new user interface, LAS X.next, for the DVM6 digital microscope. The video demonstrates how to make a fast Z-Stack with a few…
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What is a Spectral Detector (SP Detector)?
The SP detector from Leica Microsystems denotes a compound detection unit for point scanning microscopes, in particular confocal microscopes. The SP detector splits light into up to 5 spectral bands.…
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What is a Resonant Scanner?
A resonant scanner is a type of galvanometric mirror scanner that allows fast image acquisition with single-point scanning microscopes (true confocal and multiphoton laser scanning). High acquisition…
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Improve 3D Cell Biology Workflow with Light Sheet Microscopy
Understanding the sub-cellular mechanisms in carcinogenesis is of crucial importance for cancer treatment. Popular cellular models comprise cancer cells grown as monolayers. But this approach…
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What is a Field-of-View Scanner?
A field-of-view scanner is an assembly of galvanometric scanning mirrors used in single-point confocal microscopes that offer the correct optical recording of large field sizes. The field-of-view…
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Resolved Field Number (RFN)
The field number (FN) for optical microscopes indicates the field of view (FOV). It corresponds to the area in the intermediate image that is observable through the eyepieces. Although, we cannot…
