Leica LMD6 e LMD7 Microdissecação a laser
Perfeição de dissecação
This list presents current publications in the major application fields for laser microdissection and is updated monthly.
Laser microdissection is a relatively new technique, having first been developed in the 1990s. It makes it possible to obtain homogenous, ultrapure samples from heterogenous starting material. A researcher can selectively and routinely analyze specific regions of interest, down to single cells, to obtain results that are reproducible. Laser microdissection uses a microscope to visualize individual cells or cell clusters. Regions of interest are selected via software, excised from the surrounding tissue by a laser, and collected by gravity into specialized devices for analysis.
The article by Mund et al. on Deep Visual Proteomics makes the cover of Nature Biotechnology for the month of August 2022.
Download the complete list of LMD publications in "Downloads"
Single Cell Applications
Deciphering functional tumor–immune crosstalk through highly multiplexed imaging and deep visual proteomics
Zheng X, Mund A, Mann M
bioRxiv preprint, May 23, 2024
The isolation strategy and chemical analysis of oil cells from Asari Radix et Rhizoma
Hu H, Liu G, Li Y
Plant Methods. 2024 May 17;20(1):72. doi: 10.1186/s13007-024-01184-5.
Deep Visual Proteomics advances human colon organoid models by revealing a switch to an in vivo-like phenotype upon xenotransplantation
Post F, Hausmann A, Kabatnik S, Steigerwald S, Brand A, Clement DL, Skov J, Boyce TL, Sato T, Steenholdt C, Mund A, Nielsen OH, Jensen KB, Mann M
bioRxiv preprint, doi: doi.org/10.1101/2024.05.13.593888; May 14, 2024
DNA Research
Genetic and epigenetic alterations in precursor lesions of endometrial endometrioid carcinoma
Gotoh O, Sugiyama Y, Tonooka A, Kosugi M, Kitaura S, Minegishi R, Sano M, Amino S, Furuya R, Tanaka N, Kaneyasu T, Kumegawa K, Abe A, Nomura H, Takazawa Y, Kanao H, Maruyama R, Noda T, Mori S
J Pathol. 2024 May 11. doi: 10.1002/path.6278.
Integrative molecular and spatial analysis reveals evolutionary dynamics and tumor-immune interplay of in situ and invasive acral melanoma
Liu H, Gao J, Feng M, Cheng J, Tang Y, Cao Q, Zhao Z, Meng Z, Zhang J, Zhang G, Zhang C, Zhao M, Yan Y, Wang Y, Xue R, Zhang N, Li H
Cancer Cell. 2024 May 9:S1535-6108(24)00136-3. doi: 10.1016/j.ccell.2024.04.012.
Targeting pathological cells with senolytic drugs reduces seizures in neurodevelopmental mTOR-related epilepsy
Ribierre T, Bacq A, Donneger F, Doladilhe M, Maletic M, Roussel D, Le Roux I, Chassoux F, Devaux B, Adle-Biassette H, Ferrand-Sorbets S, Dorfmüller G, Chipaux M, Baldassari S, Poncer JC, Baulac S
Nat Neurosci. 2024 May 6. doi: 10.1038/s41593-024-01634-2.
RNA Research
Cytomegalovirus infection lengthens the cell cycle of granule cell precursors during postnatal cerebellar development
Sung CYW, Li M, Jonjic S, Sanchez V, Britt WJ
JCI Insight. 2024 Jun 10;9(11):e175525. doi: 10.1172/jci.insight.175525.
Myeloid cannabinoid CB1 receptor deletion confers atheroprotection in male mice by reducing macrophage proliferation in a sex-dependent manner
Wang Y, Li G, Chen B, Shakir G, Volz M, van der Vorst EPC, Maas SL, Geiger M, Jethwa C, Bartelt A, Li Z, Wettich J, Sachs N, Maegdefessel L, Nazari Jahantigh M, Hristov M, Lacy M, Lutz B, Weber C, Herzig S, Guillamat Prats R, Steffens S
Cardiovasc Res. 2024 Jun 5:cvae125. doi: 10.1093/cvr/cvae125.
Improved leptin sensitivity and increased soluble leptin receptor concentrations may underlie the additive effects of combining PYY (3-36) and exendin-4 on body weight lowering in diet-induced obese mice
Wulff BS, Kuhre RE, Selvaraj M, Rehfeld JF, Niss K, Fels JJ, Secher A, Raun K, Gerstenberg MK
HELIYON, 27. May 2024.
Highlights 2023: SPARCS (spatially resolved CRISPR screening)
Schmacke et al.
https://doi.org/10.1101/2023.06.01.542416
Proteomics, Lipidomics, Metabolomics
Plasma and Kidney Proteome Profiling Combined with Laser Capture Microdissection Reveal Large Increases in Immunoglobulins with Age
Chan LJG; Olsson N; Preciado López M; Hake K; Tomono H; Veras MA; McAllister FE
Proteomes 2024; 12, 16. 3. June 2024.
Deciphering functional tumor–immune crosstalk through highly multiplexed imaging and deep visual proteomics
Zheng X, Mund A, Mann M
bioRxiv preprint, May 23, 2024
Spatial omics of acute myocardial infarction reveals a novel mode of immune cell infiltration
Wünnemann F, Sicklinger F, Bestak K, Nimo J, Thiemann T, Amrute J, Nordbeck M, Hartmann N, Ibarra-Arellano MA, Tanevski J, Heine C, Frey N, Lavine KJ, Coscia F, Saez-Rodriguez J, Leuschner F, Schapiro D
bioRxiv preprint, May 21, 2024
Highlight 2022: Deep Visual Proteomics defines single-cell identity and heterogeneity
https://www.leica-microsystems.com/science-lab/life-science/precise-spatial-proteomic-information-in-tissues/
On single cell level:
Rosenberger FA et al. (scDVP):
https://doi.org/10.1038/s41592-023-02007-6
Original Paper:
Mund et al.
https://www.nature.com/articles/s41587-022-01302-5
Title page Nature Biotechnology, August 2022
https://www.nature.com/nbt/volumes/40/issues/8
Highlights 2023: scDVP applied
Nordmann et al.
https://doi.org/10.1101/2023.11.11.23295492
Schweizer et al.
https://doi.org/10.1101/2023.11.13.23298409
Fluorescence Applications
Deciphering functional tumor–immune crosstalk through highly multiplexed imaging and deep visual proteomics
Zheng X, Mund A, Mann M
bioRxiv preprint, May 23, 2024
The isolation strategy and chemical analysis of oil cells from Asari Radix et Rhizoma
Hu H, Liu G, Li Y
Plant Methods. 2024 May 17;20(1):72. doi: 10.1186/s13007-024-01184-5.
Deep Visual Proteomics advances human colon organoid models by revealing a switch to an in vivo-like phenotype upon xenotransplantation
Post F, Hausmann A, Kabatnik S, Steigerwald S, Brand A, Clement DL, Skov J, Boyce TL, Sato T, Steenholdt C, Mund A, Nielsen OH, Jensen KB, Mann M
bioRxiv preprint, doi: doi.org/10.1101/2024.05.13.593888; May 14, 2024
Cancer Research
Integrating artificial intelligence-based computational pathology approaches with laser microdissection for tumor enrichment and cancer research
Chain DC
Thesis, 2024
Proteogenomic characterization of difficult-to-treat breast cancer with tumor cells enriched through laser microdissection
Raj-Kumar PK, Lin X, Liu T, Sturtz LA, Gritsenko MA, Petyuk VA, Sagendorf TJ, Deyarmin B, Liu J, Praveen-Kumar A, Wang G, McDermott JE, Shukla AK, Moore RJ, Monroe ME, Webb-Robertson BM, Hooke JA, Fantacone-Campbell L, Mostoller B, Kvecher L, Kane J, Melley J, Somiari S, Soon-Shiong P, Smith RD, Mural RJ, Rodland KD, Shriver CD, Kovatich AJ, Hu H
Breast Cancer Res. 2024 May 14;26(1):76. doi: 10.1186/s13058-024-01835-4.
3D genomic mapping reveals multifocality of human pancreatic precancers
Braxton AM, Kiemen AL, Grahn MP, Forjaz A, Parksong J, Mahesh Babu J, Lai J, Zheng L, Niknafs N, Jiang L, Cheng H, Song Q, Reichel R, Graham S, Damanakis AI, Fischer CG, Mou S, Metz C, Granger J, Liu XD, Bachmann N, Zhu Y, Liu Y, Almagro-Pérez C, Jiang AC, Yoo J, Kim B, Du S, Foster E, Hsu JY, Rivera PA, Chu LC, Liu F, Fishman EK, Yuille A, Roberts NJ, Thompson ED, Scharpf RB, Cornish TC, Jiao Y, Karchin R, Hruban RH, Wu PH, Wirtz D, Wood LD
Nature. 2024 May 1. doi: 10.1038/s41586-024-07359-3.
Neurosciences
Improved leptin sensitivity and increased soluble leptin receptor concentrations may underlie the additive effects of combining PYY (3-36) and exendin-4 on body weight lowering in diet-induced obese mice
Wulff BS, Kuhre RE, Selvaraj M, Rehfeld JF, Niss K, Fels JJ, Secher A, Raun K, Gerstenberg MK
HELIYON, 27. May 2024.
Proteomic dissection of vanishing white matter pathogenesis
Man JHK, Zarekiani P, Mosen P, de Kok M, Debets DO, Breur M, Altelaar M, van der Knaap MS, Bugiani M
Cell Mol Life Sci. 2024 May 24;81(1):234. doi: 10.1007/s00018-024-05258-4.
Mapping dynamic molecular changes in hippocampal subregions after traumatic brain injury through spatial proteomics
Maity S, Huang Y, Kilgore MD, Thurmon AN, Vaasjo LO, Galazo MJ, Xu X, Cao J, Wang X, Ning B, Liu N, Fan J
Clin Proteomics. 2024 May 12;21(1):32. doi: 10.1186/s12014-024-09485-6.
Clinical/Pathology/Immunology Research Applications
Myeloid cannabinoid CB1 receptor deletion confers atheroprotection in male mice by reducing macrophage proliferation in a sex-dependent manner
Wang Y, Li G, Chen B, Shakir G, Volz M, van der Vorst EPC, Maas SL, Geiger M, Jethwa C, Bartelt A, Li Z, Wettich J, Sachs N, Maegdefessel L, Nazari Jahantigh M, Hristov M, Lacy M, Lutz B, Weber C, Herzig S, Guillamat Prats R, Steffens S
Cardiovasc Res. 2024 Jun 5:cvae125. doi: 10.1093/cvr/cvae125.
Trans-eyelid distribution of epinastine to the conjunctiva following eyelid application in rabbits
Mochizuki T, Hata T, Mori N, Yamazaki T, Noto T, Mano H
Jpn J Ophthalmol. 2024 May 25. doi: 10.1007/s10384-024-01070-6.
Spatial omics of acute myocardial infarction reveals a novel mode of immune cell infiltration
Wünnemann F, Sicklinger F, Bestak K, Nimo J, Thiemann T, Amrute J, Nordbeck M, Hartmann N, Ibarra-Arellano MA, Tanevski J, Heine C, Frey N, Lavine KJ, Coscia F, Saez-Rodriguez J, Leuschner F, Schapiro D
bioRxiv preprint, May 21, 2024
Developmental Research, Epigenetics & Aging
Cytomegalovirus infection lengthens the cell cycle of granule cell precursors during postnatal cerebellar development
Sung CYW, Li M, Jonjic S, Sanchez V, Britt WJ
JCI Insight. 2024 Jun 10;9(11):e175525. doi: 10.1172/jci.insight.175525.
Plasma and Kidney Proteome Profiling Combined with Laser Capture Microdissection Reveal Large Increases in Immunoglobulins with Age
Chan LJG; Olsson N; Preciado López M; Hake K; Tomono H; Veras MA; McAllister FE
Proteomes 2024; 12, 16. 3. June 2024.
P62 promotes FSH-induced antral follicle formation by directing degradation of ubiquitinated WT1
Zhao T, He M, Zhu Z, Zhang T, Zheng W, Qin S, Gao M, Wang W, Chen Z, Han J, Liu L, Zhou B, Wang H, Zhang H, Xia G, Wang J, Wang F, Wang C
Cell Mol Life Sci. 2024 May 20;81(1):221. doi: 10.1007/s00018-024-05251-x.
Plant Research
The isolation strategy and chemical analysis of oil cells from Asari Radix et Rhizoma
Hu H, Liu G, Li Y
Plant Methods. 2024 May 17;20(1):72. doi: 10.1186/s13007-024-01184-5.
LMD and LC-MS-based chemical constituents and pharmacological effects assessment for two different processing methods of the root of Paeonia lactiflora Pall
Xu C, Wang X, Han J, Gu Z, Guo Q
Journal of Pharmaceutical and Biomedical Analysis, 27 April 2024, 116184
Transcriptomic analyses to summarize gene expression patterns that occur during leaf initiation of Chinese cabbage
Sun XX, Liu Z, Liu R, Bucher J, Zhao J, Visser RGF, Bonnema G:
Horticulture Research, 2024
Forensics
Development of a Simple Cell Harvesting Method to Maximise DNA Recovery from Historic Microscope Slides for Sexual Assault Investigations
Hymus CM, Egan SE and Tay JW
Forensic Sci. 2022, 2(4), 795-807
Population Genetic Issues for Forensic DNA Profiles
Rhodes C
NCJRS, Document Number: 304612, Date Received: April 2022, Award Number: 2017-DN-BX-0136
Sperm Cell Capture Based on ABH Antigen Differences to Separate Two Men in Mixed Seminal Stains
Sun M, Zheng J, Wnag B and Yao J
BioMed Research International, Volume 2021 | Article ID 7269237
General Information:
http://projects.nfstc.org/tech_transition/lmd/examiners/
Further information on Leica LMD impact at forensics:
http://www.fox23.com/news/news/local/new-dna-lab-brings-hope-victims-families/nj97Z/
Miscellaneous
Integrating artificial intelligence-based computational pathology approaches with laser microdissection for tumor enrichment and cancer research
Chain DC
Thesis, 2024
Optimizing neurotransmitter pathway detection by IR-MALDESI-MSI in mouse brain
Wang MF, Ouyang Y, Segura T, Muddiman DC
Anal Bioanal Chem. 2024 Jun 1. doi: 10.1007/s00216-024-05354-1.
Paternal microbiome perturbations impact offspring fitness
Argaw-Denboba A, Schmidt TSB, Di Giacomo M, Ranjan B, Devendran S, Mastrorilli E, Lloyd CT, Pugliese D, Paribeni V, Dabin J, Pisaniello A, Espinola S, Crevenna A, Ghosh S, Humphreys N, Boruc O, Sarkies P, Zimmermann M, Bork P, Hackett JA
Nature. 2024 May 1. doi: 10.1038/s41586-024-07336-w.
Live Cell Culture
A novel method for the isolation of single cells mimicking circulating tumour cells adhered on Smart Bio Surface slides by Laser Capture Microdissection
Visci G, Tolomeo D, Lonoce A, Arshadi A, Bascetta L, Trotta G, van Riel M, Vermeesch JR, Carbone R, Storlazzi CT
Protocols.io, 26 Oct 2023
SPARCS, a platform for genome-scale CRISPR screening for spatial cellular phenotypes
Schmacke NA, Mädler SC, Wallmann G, Metousis A, Bérouti M, Harz H, Leonhardt H, Mann M, Horning V
bioRxiv, 1 June 2023, DOI: 10.1101/2023.06.01.542416
An efficient semi–in vivo zygotic embryogenesis system in Arabidopsis
Li J, Li SS, Liu Y, Tang WY, Liu XF, Meng CF, Zhang LY, Sun MX
In Vitro Cell.Dev.Biol.-Plant, 27. Dec 2022