Viventis Deep Microscópio de fluorescência Lightsheet de visão dupla

Functional in toto imaging

This list presents recent publications in the main application areas of light sheet imaging with the Viventis LS1, LS2 and Deep and is updated regularly.

Light sheet microscopy, also known as Selective Plane Illumination Microscopy (SPIM), has emerged as a powerful imaging technique in scientific research, especially for the study of living samples. This non-invasive method combines optical sectioning and low phototoxicity, allowing researchers to visualize biological processes in three dimensions with high spatiotemporal resolution. Over the years, light sheet microscopy has evolved from its early development in the late 1990s to become a cornerstone technique in several fields of study. Light sheet microscopy has transformed our understanding of developmental biology, neurobiology, and cancer research, among others, with unprecedented insights into the dynamic interactions of cells and tissues.

Publications by application

Organoids and 3D cell culture

Tuft cells act as regenerative stem cells in the human intestine
Huang et al. 
Nature 2024

Mouse neural tube organoids self-organize floorplate through BMP-mediated cluster competition
Krammer et al.
Developmental Cell 2024

NOTCH-driven oscillations control cell fate decisions during intestinal homeostasis
Weterings et al. 
BioRxiv (2024). doi.org/10.1101/2024.08.26.609553 

Control of lumen geometry and topology by the interplay between pressure and cell proliferation rate in pancreatic organoids
Lee et al.
BioRxiv 2024

The G1/S transition in mammalian stem cells in vivo is autonomously regulated by cell size
Xie et al.
BioRxiv 2024

Open top multi sample dual view light sheet microscope for live imaging of large multicellular systems
Moos et al.
Nature Methods 2024.

Decoding morphogen patterning of human neural organoids with a multiplexed single-cell transcriptomic screen
Sanchís-Calleja et al.
BioRxiv 2024

Integrating single-cell imaging and RNA sequencing datasets links differentiation and morphogenetic dynamics of human pancreatic endocrine progenitors 
Beydag-Tasöz et al.
Developmental Cell 2023 

Dynamics and plasticity of stem cells in the regenerating human colonic epithelium
Oost et al.
BioRvix 2023

Morphodynamics of human early brain organoid development
Jain et al.
BioRvix 2023 

Topological morphogenesis of neuroepithelial organoids
Ishihara et al.
Nature Physics 2022 

Multiscale light-sheet organoid imaging framework 
de Medeiros et al. 
Nature Communication 2022 

Cell fate coordinates mechano-osmotic forces in intestinal crypt formation
Yang et al.
Nature Cell Biology 2021 

Lineage recording in human cerebral organoids 
He
Nature Methods 2021 

Tissue Engineering with Mechanically Induced Solid-Fluid Transitions
Mailand et al. 
Ad. Materials 2021 

Capturing Cardiogenesis in Gastruloids
Rossi et al.
Cell Stem Cell 2021  

Self-organization and symmetry breaking in intestinal organoids development
Serra et al. 
Nature 2019 

Oocytes and Embryos Development

Inverse blebs operate as hydraulic pumps during mouse blastocyst formation
Schliffka  et al. 
Nature Cell Biology 2024 

Spatio-temporal requirements of Aurora kinase A in mouse oocytes meiotic spindle building
Blengini et al.
iScience 2024

Live imaging human embryos reveals mitotic errors and lineage specification prior to implantation
Abdelbaki et al.
BioRxiv 2024

Reconstitution of chromatin reorganization during mammalian oocyte development
Wang et al.
BioRxiv 2024

Live-imaging reveals Coordinated Cell Migration and Cardiac Fate Determination 2 during Mammalian Gastrulation
Abukar et al.
BioRxiv 2023

CHK1-CDC25A-CDK1 regulate cell cycle progression and protect genome integrity in early mouse embryos
Knoblochova et al.
Embo Reports 2023

Actin-driven chromosome clustering facilitates fast and complete chromosome capture in mammalian oocytes 
Harasimov et al.
Nature Cell Biology 2023

Cell fragmentation in mouse preimplantation embryos induced by ectopic activation of the polar body extrusion pathway
Pelzer et al.
Embo Journal 2023

Mechanism of spindle pole organization and instability in human oocytes
So et al.
Science 2022 

Aurora kinase A is essential for meiosis in mouse oocytes
Blengini et al.
Plos Genetics 2021

Hydraulic fracturing and active coarsening position the lumen of the mouse blastocyst
Dumortier et al.
Science 2019

Primed Track, high-fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins
Welling et al.
Elife 2019

Zebrafish Research

LiverZap: a chemoptogenetic tool for global and locally restricted hepatocyte ablation to study cellular behaviours in liver regeneration
Ambrosio et al.
Development 2024

Cell-autonomous generation of the wave pattern within the vertebrate segmentation clock 
Rohde et al.
eLife 2024

Clock driven waves of Tbx6 expression prefigure somite boundaries
Venzin et al.
BioRxiv 2023

Deconstructing body axis morphogenesis in zebrafish embryos using robot-assisted tissue micromanipulation
Ozelci et al.
Nature Communications 2022

Left-right symmetry of zebrafish embryos requires somite surface tension
Naganathan et al.
Nature 2022 

Mitochondria in Embryogenesis: An Organellogenesis Perspective
Arribat et al.
Frontiers in Cell and Developmental Biology 2019

Marine Organisms

Life-cycle-coupled evolution of mitosis in close relatives of animals
Shah et al.
Nature 2024

The nuclear-to-cytoplasmic ratio drives cellularization in the close animal relative Sphaeroforma arctica 
Marine Olivetta et al.
Current Biology 2023